Human CD45 Alexa Fluor® 594-conjugated Antibody

  
  • Species Reactivity
    Human
  • Specificity
    Detects human CD45. This antibody recognizes all isoforms of human CD45.
  • Source
    Monoclonal Mouse IgG1 Clone # 2D1
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Human peripheral blood mononuclear cells
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
    Alexa Fluor 594
Applications
  •  
    Recommended
    Concentration
    Sample
  • Flow Cytometry
    5 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of CD45 in Human Blood Lymphocytes by Flow Cytometry. Human peripheral blood lymphocytes were stained with Mouse Anti-Human CD45 Alexa Fluor® 594‑conjugated Monoclonal Antibody (Catalog # FAB1430T, filled histogram) or isotype control antibody (Catalog # IC002T, open histogram). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: CD45

CD45, previously called LCA (leukocyte common antigen), T200, or Ly5 in mice, is member C of the class 1 (receptor‑like) protein tyrosine phosphatase family (PTPRC) (1, 2). It is a variably glycosylated 180‑220 kDa transmembrane protein that is abundantly expressed on all nucleated cells of hematopoietic origin (1‑3). CD45 has several isoforms, expressed according to cell type, developmental stage and antigenic exposure (1‑5). The longest form, CD45RABC (called B220 in mouse), is expressed on B lymphocytes (5). The CD45RABC cDNA encodes 1304 amino acids (aa), including a 23 aa signal sequence, a 552 aa extracellular domain containing the splicing region, a cysteine‑rich region and two fibronectin type III domains, a 22 aa transmembrane sequence, and a 707 aa cytoplasmic domain that contains two phosphatase domains, D1 and D2. Only D1 has phosphatase activity. CD45R0 is the shortest form, lacking exons 4, 5 and 6 which encode aa 32‑191. It is expressed on memory cells, while intermediate sizes are expressed on other T cells (3, 4, 6). CD45 has been best studied in T cells, where it determines T cell receptor signaling thresholds (3, 6‑8). CD45 is moved into or out of the immunological synapse (IS) membrane microdomain depending on the relative influence of interaction with the extracellular galectin lattice or the intracellular actin cytoskeleton (9, 10). Galectin interaction can be fine‑tuned by varying usage of the heavily O‑glycosylated spliced regions and sialylation of N‑linked carbohydrates (4, 9). Within the IS, CD45 dephosphorylates and negatively regulates the Src family kinase, Lck (8‑10). In other leukocytes, CD45 influences differentiation and links immunoreceptor signaling with cytokine secretion and cell survival, partially overlapping in function with DEP‑1/CD148 (11‑14). CD45 deletion causes in severe immunodeficiency, while point mutations may be associated with autoimmune disorders (6, 7).

  • References:
    1. Anderson, J.N. et al. (2004) FASEB J. 18:8.
    2. Streuli, M. et al. (1987) J. Exp. Med. 166:1548.
    3. Hermiston, M.L. et al. (2003) Annu. Rev. Immunol. 21:107.
    4. Earl, L.A. and L.G. Baum (2008) Immunol. Cell Biol. 86:608.
    5. Ralph, S.J. et al. (1987) EMBO J. 6:1251.
    6. Falahti, R. and D. Leitenberg (2008) J. Immunol. 181:6082.
    7. Tchilian, E.Z. and P.C.L. Beverley (2006) Trends Immunol. 27:146.
    8. McNiell, L. et al. (2007) Immunity 27:425.
    9. Chen, I-J. et al. (2007) J. Biol. Chem. 282:35361.
    10. Freiberg, B.A. et al. (2002) Nat. Immunol. 3:911.
    11. Zhu, J.W. et al. (2008) Immunity 28:183.
    12. Huntington, N.D. et al. (2006) Nat. Immunol. 7:190.
    13. Hesslein, D.G. et al. (2006) Proc. Natl. Acad. Sci. USA 103:7012.
    14. Cross, J.L. et al. (2008) J. Immunol. 180:8020.
  • Long Name:
    Cluster of Differentiation 45
  • Entrez Gene IDs:
    5788 (Human); 19264 (Mouse); 490255 (Canine); 100061950 (Equine)
  • Alternate Names:
    B220; CD45 antigen; CD45; CD45R; EC 3.1.3.48; LCA; L-CA; LY5; protein tyrosine phosphatase, receptor type, C; PTPRC; receptor-type tyrosine-protein phosphatase C; T200 Glycoprotein; T200 leukocyte common antigen; T200receptor type, c polypeptide
Related Research Areas
Isotype Controls
Description Application Cat# Citations Images  

Mouse IgG1 Alexa Fluor® 594-conjugated Isotype Control

Ctrl IC002T
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Staining Reagents
Description Application Cat# Citations Images  

Flow Cytometry Staining Buffer (1X)

Flow FC001 4
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Flow Cytometry Mouse Lyse Buffer (10X)

Flow FC003 2
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Flow Cytometry Human Lyse Buffer (10X)

Flow FC002 1
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