Human CD45 Biotinylated Antibody

(3 citations)   
  • Species Reactivity
    Human
  • Specificity
    Detects human CD45. This antibody recognizes all isoforms of human CD45.
  • Source
    Monoclonal Mouse IgG1 Clone # 2D1
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Human peripheral blood mononuclear cells
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
  • Label
    Biotin
Applications
  •  
    Recommended
    Concentration
    Sample
  • Flow Cytometry
    2.5 µg/106 cells
    Human peripheral blood mononuclear cells
  • Immunocytochemistry
    8-25 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Immunocytochemistry
CD45 in human PBMCs. CD45 was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) using Mouse Anti-Human CD45 Biotinylated Monoclonal Antibody (Catalog # BAM1430) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Streptavidin (red; Catalog # NL999) and counterstained with DAPI (blue). Specific staining was localized to plasma membrane. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CD45

CD45, previously called LCA (leukocyte common antigen), T200, or Ly5 in mice, is member C of the class 1 (receptor‑like) protein tyrosine phosphatase family (PTPRC) (1, 2). It is a variably glycosylated 180‑220 kDa transmembrane protein that is abundantly expressed on all nucleated cells of hematopoietic origin (1‑3). CD45 has several isoforms, expressed according to cell type, developmental stage and antigenic exposure (1‑5). The longest form, CD45RABC (called B220 in mouse), is expressed on B lymphocytes (5). The CD45RABC cDNA encodes 1304 amino acids (aa), including a 23 aa signal sequence, a 552 aa extracellular domain containing the splicing region, a cysteine‑rich region and two fibronectin type III domains, a 22 aa transmembrane sequence, and a 707 aa cytoplasmic domain that contains two phosphatase domains, D1 and D2. Only D1 has phosphatase activity. CD45R0 is the shortest form, lacking exons 4, 5 and 6 which encode aa 32‑191. It is expressed on memory cells, while intermediate sizes are expressed on other T cells (3, 4, 6). CD45 has been best studied in T cells, where it determines T cell receptor signaling thresholds (3, 6‑8). CD45 is moved into or out of the immunological synapse (IS) membrane microdomain depending on the relative influence of interaction with the extracellular galectin lattice or the intracellular actin cytoskeleton (9, 10). Galectin interaction can be fine‑tuned by varying usage of the heavily
O-glycosylated spliced regions and sialylation of N‑linked carbohydrates (4, 9). Within the IS, CD45 dephosphorylates and negatively regulates the Src family kinase, Lck (8‑10). In other leukocytes, CD45 influences differentiation and links immunoreceptor signaling with cytokine secretion and cell survival, partially overlapping in function with DEP‑1/CD148 (11‑14). CD45 deletion causes in severe immunodeficiency, while point mutations may be associated with autoimmune disorders (6, 7).

  • References:
    1. Anderson, J.N. et al. (2004) FASEB J. 18:8.
    2. Streuli, M. et al. (1987) J. Exp. Med. 166:1548.
    3. Hermiston, M.L. et al. (2003) Annu. Rev. Immunol. 21:107.
    4. Earl, L.A. and L.G. Baum (2008) Immunol. Cell Biol. 86:608.
    5. Ralph, S.J. et al. (1987) EMBO J. 6:1251.
    6. Falahti, R. and D. Leitenberg (2008) J. Immunol. 181:6082.
    7. Tchilian, E.Z. and P.C.L. Beverley (2006) Trends Immunol. 27:146.
    8. McNiell, L. et al. (2007) Immunity 27:425.
    9. Chen, I-J. et al. (2007) J. Biol. Chem. 282:35361.
    10. Freiberg, B.A. et al. (2002) Nat. Immunol. 3:911.
    11. Zhu, J.W. et al. (2008) Immunity 28:183.
    12. Huntington, N.D. et al. (2006) Nat. Immunol. 7:190.
    13. Hesslein, D.G. et al. (2006) Proc. Natl. Acad. Sci. USA 103:7012.
    14. Cross, J.L. et al. (2008) J. Immunol. 180:8020.
  • Long Name:
    Cluster of Differentiation 45
  • Entrez Gene IDs:
    5788 (Human); 19264 (Mouse); 490255 (Canine); 100061950 (Equine)
  • Alternate Names:
    B220; CD45 antigen; CD45; CD45R; EC 3.1.3.48; LCA; L-CA; LY5; protein tyrosine phosphatase, receptor type, C; PTPRC; receptor-type tyrosine-protein phosphatase C; T200 Glycoprotein; T200 leukocyte common antigen; T200receptor type, c polypeptide
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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Species
Applications
Sample Type
  1. Expression of ?-globin by cancer cells promotes cell survival during blood-borne dissemination
    Authors: Y Zheng, DT Miyamoto, BS Wittner, JP Sullivan, N Aceto, NV Jordan, M Yu, NM Karabacak, V Comaills, R Morris, R Desai, N Desai, E Emmons, JD Milner, RJ Lee, CL Wu, LV Sequist, W Haas, DT Ting, M Toner, S Ramaswamy, S Maheswaran, DA Haber
    Nat Commun, 2017;8(0):14344.
    Species: Human
    Sample Type: Whole Blood
    Application: Bioassay
  2. HER2 expression identifies dynamic functional states within circulating breast cancer cells
    Nature, 2016;0(0):.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  3. Increased Susceptibility of Humanized NSG Mice to Panton-Valentine Leukocidin and Staphylococcus aureus Skin Infection.
    Authors: Tseng C, Biancotti J, Berg B, Gate D, Kolar S, Muller S, Rodriguez M, Rezai-Zadeh K, Fan X, Beenhouwer D, Town T, Liu G
    PLoS Pathog, 2015;11(11):e1005292.
    Species: Human
    Sample Type: Whole Tissue
    Application: IHC - Paraffin embedded
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