Human CD82/Kai-1 Antibody

Catalog # Availability Size / Price Qty
Detection of CD82/Kai‑1 in Human Granulocytes by Flow Cytometry.
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Product Details
Citations (2)
Supplemental Products
Reviews (4)

Human CD82/Kai-1 Antibody Summary

Species Reactivity
Detects human CD82/Kai‑1. Stains human CD82/Kai‑1 transfectants but not irrelevant transfectants.
Monoclonal Mouse IgG1 Clone # 423524
Protein A or G purified from hybridoma culture supernatant
NS0 mouse myeloma cell line transfected with human CD82/Kai‑1
Accession # AAH01821
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.


Recommended Concentration
Flow Cytometry
2.5 µg/106 cells
See below
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of CD82/Kai‑1 antibody in Human Granulocytes antibody by Flow Cytometry. View Larger

Detection of CD82/Kai‑1 in Human Granulocytes by Flow Cytometry. Human whole blood granulocytes were stained with Mouse Anti-Human CD82/Kai-1 Monoclonal Antibody (Catalog # MAB4616, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG F(ab')2Secondary Antibody (Catalog # F0102B).

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Preparation and Storage

Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CD82/Kai-1

CD82, also known as KAI1, C33 and TSPAN27, is a widely expressed palmitoylated molecule in the tetraspanin superfamily. CD82 is synthesized as a 28 kDa core protein with mature molecular weights ranging up to 70 kDa due to variable glycosylation. CD82 functions as a suppressor of metastasis and is down‑regulated in many malignant tumors. It associates with IGSF8, DARC, the ganglioside GM2, and the HTLV-1 Gag protein. CD82 inhibits cell migration and metastasis by preventing integrin maturation and blocking integrin interactions with Met. Human CD82 shares 76% aa sequence identity with mouse and rat CD82.

Entrez Gene IDs
3732 (Human); 12521 (Mouse); 83628 (Rat)
Alternate Names
CD82 antigenC33; CD82 molecule; CD82; IA4; IA4C33 antigen; Inducible membrane protein R2; KAI1; KAI1GR15; kangai 1 (suppression of tumorigenicity 6, prostate; CD82 antigen (R2 leukocyteantigen, antigen detected by monoclonal and IA4)); Metastasis suppressor Kangai-1; R2; SAR2; SAR2tetraspanin-27; ST6; ST6tspan-27; Suppressor of tumorigenicity 6 protein; Tetraspanin-27; TSPAN27; Tspan-27; TSPAN274F9

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Citations for Human CD82/Kai-1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. Individually cultured bovine embryos produce extracellular vesicles that have the potential to be used as non-invasive embryo quality markers
    Authors: K Dissanayak, M Nõmm, F Lättekivi, Y Ressaissi, K Godakumara, A Lavrits, G Midekessa, J Viil, R Bæk, MM Jørgensen, S Bhattachar, A Andronowsk, A Salumets, Ü Jaakma, A Fazeli
    Theriogenology, 2020;149(0):104-116.
    Species: Mouse
    Sample Types:
    Applications: Microarray
  2. The DSCs-expressed CD82 controls the invasiveness of trophoblast cells via integrinbeta1/MAPK/MAPK3/1 signaling pathway in human first-trimester pregnancy.
    Authors: Li MQ, Hou XF, Shao J, Tang CL, Li DJ
    Biol. Reprod., 2010;82(5):968-79.
    Species: Human
    Sample Types: Whole Cells
    Applications: Cell-based ELISA


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Reviews for Human CD82/Kai-1 Antibody

Average Rating: 4.3 (Based on 4 Reviews)

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Human CD82/Kai-1 Antibody
By Anonymous on 06/10/2020
Application: Microarrays Sample Tested: EDTA Plasma Species: Human

Human CD82/Kai-1 Antibody
By Anonymous on 02/08/2020
Application: MiAr Sample Tested: EDTA Plasma Species: Human

Antibody was printed on custom arrays and incubated with fluorescently labeled human EDTA plasma

Human CD82/Kai-1 Antibody
By Anonymous on 11/10/2018
Application: ELISA Sample Tested: Plasma Species: Human

Human CD82/Kai-1 Antibody
By Anonymous on 11/07/2018
Application: Microarrays Sample Tested: EDTA Plasma Species: Human