Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human, Mouse

Applications

Validated:

Flow Cytometry, CyTOF-ready

Cited:

Flow Cytometry, Immunocytochemistry, Array Development, Cell-based ELISA, ELISA Capture, Microarray

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 423524
View all CD82/Kai-1 Primary Antibodies »

Product Specifications

Immunogen

NS0 mouse myeloma cell line transfected with human CD82/Kai‑1
Met1-Tyr267
Accession # AAH01821

Specificity

Detects human CD82/Kai‑1. Stains human CD82/Kai‑1 transfectants but not irrelevant transfectants.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human CD82/Kai‑1 Antibody

Detection of CD82/Kai‑1 antibody in Human Granulocytes antibody by Flow Cytometry.

Detection of CD82/Kai‑1 in Human Granulocytes by Flow Cytometry.

Human whole blood granulocytes were stained with Mouse Anti-Human CD82/Kai-1 Monoclonal Antibody (Catalog # MAB4616, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG F(ab')2Secondary Antibody (Catalog # F0102B).

Applications for Human CD82/Kai‑1 Antibody

Application
Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

2.5 µg/106 cells
Sample: Human whole blood granulocytes

Reviewed Applications

Read 4 reviews rated 4.3 using MAB4616 in the following applications:

Flow Cytometry Panel Builder

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Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: CD82/Kai-1

CD82, also known as KAI1, C33 and TSPAN27, is a widely expressed palmitoylated molecule in the tetraspanin superfamily. CD82 is synthesized as a 28 kDa core protein with mature molecular weights ranging up to 70 kDa due to variable glycosylation. CD82 functions as a suppressor of metastasis and is down‑regulated in many malignant tumors. It associates with IGSF8, DARC, the ganglioside GM2, and the HTLV-1 Gag protein. CD82 inhibits cell migration and metastasis by preventing integrin maturation and blocking integrin interactions with Met. Human CD82 shares 76% aa sequence identity with mouse and rat CD82.

Alternate Names

CD82, IA4, KAI1, SAR2, ST6, TSPAN27

Entrez Gene IDs

3732 (Human); 12521 (Mouse); 83628 (Rat)

Gene Symbol

CD82

UniProt

AAH01821

Additional CD82/Kai-1 Products

Product Documents for Human CD82/Kai‑1 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human CD82/Kai‑1 Antibody

For research use only

Citations for Human CD82/Kai‑1 Antibody

Customer Reviews for Human CD82/Kai‑1 Antibody (4)

4.3 out of 5
4 Customer Ratings
5 Stars
50%
4 Stars
25%
3 Stars
25%
2 Stars
0%
1 Stars
0%

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Customer Images

Showing  1 - 4 of 4 reviews Showing All
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  • Human CD82/Kai-1 Antibody
    Name: Anonymous
    Application: Microarrays
    Sample Tested: EDTA Plasma
    Species: Human
    Verified Customer | Posted 06/10/2020
    Human CD82/Kai‑1 Antibody MAB4616
  • Human CD82/Kai-1 Antibody
    Name: Anonymous
    Application: Microarray
    Sample Tested: EDTA Plasma
    Species: Human
    Verified Customer | Posted 02/08/2020
    Antibody was printed on custom arrays and incubated with fluorescently labeled human EDTA plasma
    Human CD82/Kai‑1 Antibody MAB4616
  • Human CD82/Kai-1 Antibody
    Name: Anonymous
    Application: ELISA
    Sample Tested: Plasma
    Species: Human
    Verified Customer | Posted 11/10/2018
  • Human CD82/Kai-1 Antibody
    Name: Anonymous
    Application: Microarrays
    Sample Tested: EDTA Plasma
    Species: Human
    Verified Customer | Posted 11/07/2018

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