Human CD8 Antibody (RPA-T8)

R&D Systems | Catalog # MAB3802

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Key Product Details

Species Reactivity

Human

Applications

Flow Cytometry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # RPA-T8
View all CD8 Primary Antibodies »

Product Specifications

Immunogen

Human CD8 alpha
Accession # NP_741696

Specificity

Detects human CD8 alpha in direct ELISAs.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human CD8 Antibody (RPA-T8)

Detection of CD8 in Human Blood Lymphocytes by Flow Cytometry.

Human peripheral blood lymphocytes were stained with (A) Mouse Anti-Human CD8 Monoclonal Antibody (Catalog # MAB3802) or (B) Mouse IgG1 Isotype Control (MAB002), followed by PE-conjugated anti-Mouse IgG secondary antibody (F0102B) and Mouse Anti-Human CD3 epsilon APC-conjugated Monoclonal Antibody (FAB100A). Staining was performed using our Staining Membrane-associated Proteins protocol.

Applications for Human CD8 Antibody (RPA-T8)

Application
Recommended Usage

Flow Cytometry

0.25 µg/106 cells
Sample: Human PBMC lymphocytes

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: CD8

CD8 is a heterodimeric glycoprotein consisting of an alpha and beta chain. It is expressed on cytolytic T cells and functions in conjunction with the T cell receptor in the recognition of MHC/peptide complexes.

Alternate Names

CD8, CD8A

Entrez Gene IDs

925 (Human); 12525 (Mouse); 24930 (Rat); 102124337 (Cynomolgus Monkey); 493799 (Feline)

Gene Symbol

CD8A

UniProt

NP_741696

Additional CD8 Products

Product Documents for Human CD8 Antibody (RPA-T8)

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human CD8 Antibody (RPA-T8)

For research use only

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