CDK8 in Human Colon.
CDK8 was detected in immersion fixed paraffin-embedded sections of human colon using Sheep Anti-Human CDK8 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6809) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to nuclei of epithelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Preparation and Storage
Sterile PBS to a final concentration of 0.2 mg/mL.
Reconstitution Buffer Available
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
CDK8 (cyclin-dependent kinase 8; also K35) is a 53-55 kDa member of the CDC2/CDKX subfamily, CMGC Ser/Thr protein kinase family of enzymes. Together with cyclin C, MED12 and MED13, CDK8 forms a "CDK8 module" that contributes to the structure of a four module Mediator complex. CDK8 would appear to have multiple functions. It can block transcription, and the re-initiation of transcription; it can also promote the transcription of thyroid hormone target genes; and it appears to phosphorylate the ICD of Notch, promoting its degradation. Human CDK8 is 464 amino acids (aa) in length. It contains one protein kinase domain (aa 21-335) and two utilized phosphorylation sites at Thr410 and Ser413. There are two potential alternative start sites at Met174 and Met61. Over aa 380-464, human CDK8 shares 98% aa identity with mouse CDK8.
Cyclin-dependent Kinase 8
Entrez Gene IDs:
1024 (Human); 264064 (Mouse)
CDK8 protein kinase; CDK8; Cell division protein kinase 8; cyclin-dependent kinase 8; EC 2.7.11; EC 184.108.40.206; EC 220.127.116.11; K35; Mediator complex subunit CDK8; Mediator of RNA polymerase II transcription subunit CDK8; MGC126074; MGC126075; Protein kinase K35
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