Human CDK8 Antibody

  
  • Species Reactivity
    Human
  • Specificity
    Detects human CDK8 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human (rh) CDK2, rhCDK4, and CDK6 is observed.
  • Source
    Polyclonal Sheep IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    E. coli-derived recombinant human CDK8
    Asn380-Tyr464
    Accession # P49336
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Immunohistochemistry
    5-15 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Immunohistochemistry
CDK8 in Human Colon. CDK8 was detected in immersion fixed paraffin-embedded sections of human colon using Sheep Anti-Human CDK8 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6809) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to nuclei of epithelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Preparation and Storage
  • Reconstitution
    Sterile PBS to a final concentration of 0.2 mg/mL.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CDK8

CDK8 (cyclin-dependent kinase 8; also K35) is a 53-55 kDa member of the CDC2/CDKX subfamily, CMGC Ser/Thr protein kinase family of enzymes. Together with cyclin C, MED12 and MED13, CDK8 forms a "CDK8 module" that contributes to the structure of a four module Mediator complex. CDK8 would appear to have multiple functions. It can block transcription, and the re-initiation of transcription; it can also promote the transcription of thyroid hormone target genes; and it appears to phosphorylate the ICD of Notch, promoting its degradation. Human CDK8 is 464 amino acids (aa) in length. It contains one protein kinase domain (aa 21-335) and two utilized phosphorylation sites at Thr410 and Ser413. There are two potential alternative start sites at Met174 and Met61. Over aa 380-464, human CDK8 shares 98% aa identity with mouse CDK8.

  • Long Name:
    Cyclin-dependent Kinase 8
  • Entrez Gene IDs:
    1024 (Human); 264064 (Mouse)
  • Alternate Names:
    CDK8 protein kinase; CDK8; Cell division protein kinase 8; cyclin-dependent kinase 8; EC 2.7.11; EC 2.7.11.22; EC 2.7.11.23; K35; Mediator complex subunit CDK8; Mediator of RNA polymerase II transcription subunit CDK8; MGC126074; MGC126075; Protein kinase K35
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Donkey Anti-Sheep IgG (H+L) PerCP-conjugated Antibody

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