Human CIDEC Antibody Summary
Accession # Q96AQ7
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of Human CIDEC by Western Blot. Western blot shows lysates of AGS human gastric adenocarcinoma cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human CIDEC Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6960) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for CIDEC at approximately 20 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
CIDEC in Human Mesenchymal Stem Cells. CIDEC was detected in immersion fixed human mesenchymal stem cells differentiated to adipocytes using Sheep Anti-Human CIDEC Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6960) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red, upper panel; Catalog # NL010) and counterstained with DAPI (blue, lower panel). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
CIDE-3 (Cell death-Inducing DNA fragmentation factor-alpha -like Effector protein 3; also CIDEC and FSP27 in mouse) is a 24-32 kDa member of the CIDE family of molecules. It is a lipid droplet stabilizer that is expressed in hepatocytes and adipocytes, and found to be associated with both mitochondria and the surface of cytoplasmic lipid droplets. CIDE-3 is unstable in the absence of triglycerides and free fatty acids, and is subject to ubiquitin-mediated degradation. In the presence of elevated fatty acids or triglycerides, CIDE-3 half-life is prolonged, and it assists in the formation of lipid droplets. Although other CIDE family members are associated with the initiation of apoptosis, it is unclear if CIDE-3 possesses such activity. Human CIDE-3 is 238 amino acids (aa) in length, and contains one CIDE domain (aa 41-120). There are multiple isoform variants. Two possesses alternative start sites, one at Met74, and another 14 aa upstream of the standard site. A third isoform shows an insertion of ten aa after Lys69, while a fourth isoform possesses a 60 aa substitution for aa 70-238. Over aa 75-176, human CIDE-3 shares 84% aa identity with mouse CIDE-3.
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