Human CIDEC Antibody

Catalog # Availability Size / Price Qty
AF6960
AF6960-SP
Detection of Human CIDEC by Western Blot.
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Human CIDEC Antibody Summary

Species Reactivity
Human
Specificity
Detects human CIDEC in direct ELISAs and Western blots. In direct ELISAs, less than 3% cross-reactivity with recombinant human CIDEA is observed.
Source
Polyclonal Sheep IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human CIDEC
Met75-His176
Accession # Q96AQ7
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below
Immunocytochemistry
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Western Blot Detection of Human CIDEC by Western Blot. View Larger

Detection of Human CIDEC by Western Blot. Western blot shows lysates of AGS human gastric adenocarcinoma cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human CIDEC Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6960) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for CIDEC at approximately 20 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunocytochemistry CIDEC in Human Mesenchymal Stem Cells. View Larger

CIDEC in Human Mesenchymal Stem Cells. CIDEC was detected in immersion fixed human mesenchymal stem cells differentiated to adipocytes using Sheep Anti-Human CIDEC Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6960) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red, upper panel; Catalog # NL010) and counterstained with DAPI (blue, lower panel). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Sterile PBS to a final concentration of 0.2 mg/mL.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CIDEC

CIDE-3 (Cell death-Inducing DNA fragmentation factor-alpha -like Effector protein 3; also CIDEC and FSP27 in mouse) is a 24-32 kDa member of the CIDE family of molecules. It is a lipid droplet stabilizer that is expressed in hepatocytes and adipocytes, and found to be associated with both mitochondria and the surface of cytoplasmic lipid droplets. CIDE-3 is unstable in the absence of triglycerides and free fatty acids, and is subject to ubiquitin-mediated degradation. In the presence of elevated fatty acids or triglycerides, CIDE-3 half-life is prolonged, and it assists in the formation of lipid droplets. Although other CIDE family members are associated with the initiation of apoptosis, it is unclear if CIDE-3 possesses such activity. Human CIDE-3 is 238 amino acids (aa) in length, and contains one CIDE domain (aa 41-120). There are multiple isoform variants. Two possesses alternative start sites, one at Met74, and another 14 aa upstream of the standard site. A third isoform shows an insertion of ten aa after Lys69, while a fourth isoform possesses a 60 aa substitution for aa 70-238. Over aa 75-176, human CIDE-3 shares 84% aa identity with mouse CIDE-3.

Long Name
Cell Death-inducing DFFA-like Effector C
Entrez Gene IDs
63924 (Human); 14311 (Mouse); 500292 (Rat)
Alternate Names
cell death activator CIDE-3; cell death-inducing DFFA-like effector c; Cell death-inducing DFFA-like effector protein C; CIDE-3; CIDEC; CIDEX; Fat-specific protein FSP27 homolog; FLJ20871; FSP27; FSP27fat specific protein 27

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