Human Coagulation Factor V (Light Chain) Antibody
Human Coagulation Factor V (Light Chain) Antibody Summary
Accession # P12259
This antibody functions as an ELISA detection antibody when paired with Rabbit Anti-Human Coagulation Factor V (Light Chain) Monoclonal Antibody (Catalog # MAB98561).
This product is intended for assay development on various assay platforms requiring antibody pairs.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of Human Coagulation Factor V in HepG2 Human Cell Line by Flow Cytometry. HepG2 human hepatocellular carcinoma cell line was stained with Rabbit Anti-Human Coagulation Factor V Monoclonal Antibody (Catalog # MAB9856, filled histogram) or isotype control antibody (MAB1050, open histogram), followed by Allophycocyanin-conjugated Anti-Rabbit IgG Secondary Antibody (F0111). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (FC005). Staining was performed using our Staining Intracellular Molecules protocol.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Coagulation Factor V
Factor V (Coagulation Factor Five) is a 330kDa protein of the coagulation system encoded by the F5 gene. Unlike most other coagulation factors, Factor V is not enzymatically active but functions as a cofactor. Factor V is a central regulator of hemostasis. It serves as a critical cofactor for the pro-thrombinase activity of factor Xa that results in the activation of pro-thrombin to thrombin.
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