Human Coagulation Factor V (Light Chain) Antibody

Recombinant Monoclonal Antibody
Catalog # Availability Size / Price Qty
MAB9856-100
MAB9856-SP

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Detection of Human Coagulation Factor V in HepG2 Human Cell Line by Flow Cytometry.
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Human Coagulation Factor V (Light Chain) Antibody Summary

Species Reactivity
Human
Specificity
Detects human Coagulation Factor V in direct ELISAs.
Source
Recombinant Monoclonal Rabbit IgG Clone # 2280A
Purification
Protein A or G purified from cell culture supernatant
Immunogen
Synthetic peptide containing human Coagulation Factor V aa1650-1700
Accession # P12259
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
ELISA

This antibody functions as an ELISA detection antibody when paired with Rabbit Anti-Human Coagulation Factor V (Light Chain) Monoclonal Antibody (Catalog # MAB98561).

This product is intended for assay development on various assay platforms requiring antibody pairs.

 
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 
Intracellular Staining by Flow Cytometry
0.25 µg/106 cells
HepG2 human hepatocellular carcinoma cell line

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Intracellular Staining by Flow Cytometry Detection of Human Coagulation Factor V antibody in HepG2 Human Cell Line antibody by Flow Cytometry. View Larger

Detection of Human Coagulation Factor V in HepG2 Human Cell Line by Flow Cytometry. HepG2 human hepatocellular carcinoma cell line was stained with Rabbit Anti-Human Coagulation Factor V Monoclonal Antibody (Catalog # MAB9856, filled histogram) or isotype control antibody (MAB1050, open histogram), followed by Allophycocyanin-conjugated Anti-Rabbit IgG Secondary Antibody (F0111). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (FC005). Staining was performed using our Staining Intracellular Molecules protocol.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Coagulation Factor V

Factor V (Coagulation Factor Five) is a 330kDa protein of the coagulation system encoded by the F5 gene. Unlike most other coagulation factors, Factor V is not enzymatically active but functions as a cofactor. Factor V is a central regulator of hemostasis. It serves as a critical cofactor for the pro-thrombinase activity of factor Xa that results in the activation of pro-thrombin to thrombin.

Long Name
Coagulation Factor V
Entrez Gene IDs
2153 (Human); 14067 (Mouse); 304929 (Rat)
Alternate Names
Activated protein C cofactor; Cf5; Cf-5; Coagulation Factor 5; coagulation factor V (proaccelerin, labile factor); coagulation factor V jinjiang A2 domain; Coagulation Factor V; F5; factor V Leiden; Factor V; FVL; PCCF; Proaccelerin, Labile Factor; RPRGL1; THPH2

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