Key Product Details

Species Reactivity

Human

Applications

ELISA, Intracellular Staining by Flow Cytometry, CyTOF-ready

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 2280A
View all Coagulation Factor V Primary Antibodies »

Product Specifications

Immunogen

Synthetic peptide containing human Coagulation Factor V aa1650-1700
Accession # P12259

Specificity

Detects human Coagulation Factor V in direct ELISAs.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Human Coagulation Factor V (Light Chain) Antibody

Detection of Human Coagulation Factor V antibody in HepG2 Human Cell Line antibody by Flow Cytometry.

Detection of Human Coagulation Factor V in HepG2 Human Cell Line by Flow Cytometry.

HepG2 human hepatocellular carcinoma cell line was stained with Rabbit Anti-Human Coagulation Factor V Monoclonal Antibody (Catalog # MAB9856, filled histogram) or isotype control antibody (MAB1050, open histogram), followed by Allophycocyanin-conjugated Anti-Rabbit IgG Secondary Antibody (F0111). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (FC005). Staining was performed using our Staining Intracellular Molecules protocol.

Applications for Human Coagulation Factor V (Light Chain) Antibody

Application
Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

ELISA

This antibody functions as an ELISA detection antibody when paired with Rabbit Anti-Human Coagulation Factor V (Light Chain) Monoclonal Antibody (Catalog # MAB98561).

This product is intended for assay development on various assay platforms requiring antibody pairs.

Intracellular Staining by Flow Cytometry

0.25 µg/106 cells
Sample: HepG2 human hepatocellular carcinoma cell line

Flow Cytometry Panel Builder

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Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from cell culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Coagulation Factor V

Factor V (Coagulation Factor Five) is a 330kDa protein of the coagulation system encoded by the F5 gene. Unlike most other coagulation factors, Factor V is not enzymatically active but functions as a cofactor. Factor V is a central regulator of hemostasis. It serves as a critical cofactor for the pro-thrombinase activity of factor Xa that results in the activation of pro-thrombin to thrombin.

Long Name

Coagulation Factor V

Alternate Names

Cf-5, Cf5, Coagulation Factor 5, F5, Factor V, FVL, PCCF, Proaccelerin, Labile Factor, RPRGL1, THPH2

Entrez Gene IDs

2153 (Human); 14067 (Mouse); 304929 (Rat)

Gene Symbol

F5

UniProt

P12259

Additional Coagulation Factor V Products

Product Documents for Human Coagulation Factor V (Light Chain) Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human Coagulation Factor V (Light Chain) Antibody

For research use only

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Protocols

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FAQs

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