Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunohistochemistry, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2B Clone # 394107
View all Complement Component C1qA Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human Complement Component C1qA
Lys110-Ala245
Accession # P02745

Specificity

Detects recombinant human Complement Component C1qA in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human Complement Component C1qB is observed.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2B

Scientific Data Images for Human Complement Component C1qA Antibody

Detection of Complement Component C1qA in Human Placenta.

Complement Component C1qA was detected in immersion fixed paraffin-embedded sections of Human Placenta using Rat Anti-Human Complement Component C1qA Monoclonal Antibody (Catalog # MAB4035) at 1 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Rat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC005). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to endothelial cells in cytotrophoblast in chorionic villi. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Applications for Human Complement Component C1qA Antibody

Application
Recommended Usage

Immunohistochemistry

1-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of Human Placenta

Western Blot

1 µg/mL
Sample: Recombinant Human Complement Component C1qA

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Complement Component C1qA

C1qA, one of three subunits of complement component C1q, contains a collagen-like domain and a globular C1q domain. Within the globular domain, human C1qA shares 73% and 77% aa sequence identity with mouse and rat C1qA, respectively. Six disulfide-linked heterodimers of C1qA and C1qB associate with three disulfide-linked homodimers of C1qC to form C1q. Deficiency in C1qA is associated with the development of autoimmunity.

Long Name

Complement Component 1q Subcomponent, A Chain

Alternate Names

C1QA

Entrez Gene IDs

712 (Human); 12259 (Mouse); 298566 (Rat)

Gene Symbol

C1QA

UniProt

P02745

Additional Complement Component C1qA Products

Product Documents for Human Complement Component C1qA Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human Complement Component C1qA Antibody

For research use only

Citations for Human Complement Component C1qA Antibody

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Protocols

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