Human Contactin-1 Antibody

Recombinant Monoclonal Antibody
Catalog # Availability Size / Price Qty
MAB9041-100
MAB9041-SP
Detection  of Human Contactin‑1 by Western Blot.
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Human Contactin-1 Antibody Summary

Species Reactivity
Human
Specificity
Detects human Contactin-1 in direct ELISAs.
Source
Recombinant Monoclonal Rabbit IgG Clone # 2332B
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Human embryonic kidney cell, HEK293-derived human Contactin-1
Met1-Ser993
Accession # Q12860
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
2 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Western Blot Detection  of Human Contactin‑1 by Western Blot. View Larger

Detection of Human Contactin‑1 by Western Blot. Western blot shows lysates of human brain tissue and A549 human lung carcinoma cell line. PVDF membrane was probed with 2 µg/mL of Rabbit Anti-Human Contactin‑1 Monoclonal Antibody (Catalog # MAB9041) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Contactin‑1 at approximately 135 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Contactin-1

Contactin-1 is a GPI-anchored adhesion protein. It is preferentially expressed in neuronal tissues and interacts with Nr-CAM and Tenascin.

Entrez Gene IDs
1272 (Human); 12805 (Mouse)
Alternate Names
CNTN1; contactin 1; Contactin1; Contactin-1; F11; F3; F3cam; Glycoprotein gp135; GP135; Neural cell surface protein F3

Product Datasheets

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