Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human, Mouse

Applications

Validated:

Western Blot, Immunocytochemistry

Cited:

Immunohistochemistry-Frozen, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Sheep IgG
View all CRX Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human CRX
Ala166-Leu299
Accession # O43186

Specificity

Detects human CRX in direct ELISAs and Western blots.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Scientific Data Images for Human CRX Antibody

Detection of Human CRX antibody by Western Blot.

Detection of Human CRX by Western Blot.

Western blot shows lysates of Y-79 human retinoblastoma cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human CRX Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7085) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for CRX at approximately 38 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

CRX antibody in Y-79 Human Cell Line by Immunocytochemistry (ICC).

CRX in Y‑79 Human Cell Line.

CRX was detected in immersion fixed Y-79 human retinoblastoma cell line using Sheep Anti-Human CRX Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7085) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red, upper panel; Catalog # NL010) and counterstained with DAPI (blue, lower panel). Specific staining was localized to nuclei and cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Applications for Human CRX Antibody

Application
Recommended Usage

Immunocytochemistry

5-15 µg/mL
Sample: Immersion fixed Y‑79 human retinoblastoma cell line

Western Blot

1 µg/mL
Sample: Y‑79 human retinoblastoma cell line

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Sterile PBS to a final concentration of 0.2 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: CRX

CRX (Cone Rod homeoboX protein) is a 37-39 kDa member of the Otx family of paired-like homeodomain containing proteins. It is expressed in pinealocytes, cones, rods and rod bipolar cells, and promotes the transcription of photoreceptor cell proteins such as rhodopsin, IRBP and beta -phosphodiesterase. Although CRX may act alone, it typically binds noncovalently to both QRX and NRL, forming a transcriptional complex. Human CRX is 299 amino acids (aa) in length. It contains one DNA‑binding homeobox domain (aa 39-98). There is one isoform variant that shows a deletion of aa 146-149. Over aa 166-299, human CRX shares 97% aa identity with mouse CRX.

Long Name

Cone-rod Homeobox Protein

Alternate Names

CORD2, LCA7, OTX3

Entrez Gene IDs

1406 (Human); 12951 (Mouse); 60446 (Rat)

Gene Symbol

CRX

UniProt

O43186 (Human)

Additional CRX Products

Product Documents for Human CRX Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human CRX Antibody

For research use only

Citations for Human CRX Antibody

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Protocols

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