Human CRX Antibody

Catalog # Availability Size / Price Qty
Detection of Human CRX by Western Blot.
2 Images
Product Details
Citations (2)
Supplemental Products

Human CRX Antibody Summary

Species Reactivity
Detects human CRX in direct ELISAs and Western blots.
Polyclonal Sheep IgG
Antigen Affinity-purified
E. coli-derived recombinant human CRX
Accession # O43186
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.


Recommended Concentration
Western Blot
1 µg/mL
See below
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Western Blot Detection of Human CRX by Western Blot. View Larger

Detection of Human CRX by Western Blot. Western blot shows lysates of Y-79 human retinoblastoma cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human CRX Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7085) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for CRX at approximately 38 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunocytochemistry CRX in Y‑79 Human Cell Line. View Larger

CRX in Y‑79 Human Cell Line. CRX was detected in immersion fixed Y-79 human retinoblastoma cell line using Sheep Anti-Human CRX Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7085) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red, upper panel; Catalog # NL010) and counterstained with DAPI (blue, lower panel). Specific staining was localized to nuclei and cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Sterile PBS to a final concentration of 0.2 mg/mL.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CRX

CRX (Cone Rod homeoboX protein) is a 37-39 kDa member of the Otx family of paired-like homeodomain containing proteins. It is expressed in pinealocytes, cones, rods and rod bipolar cells, and promotes the transcription of photoreceptor cell proteins such as rhodopsin, IRBP and beta -phosphodiesterase. Although CRX may act alone, it typically binds noncovalently to both QRX and NRL, forming a transcriptional complex. Human CRX is 299 amino acids (aa) in length. It contains one DNA‑binding homeobox domain (aa 39-98). There is one isoform variant that shows a deletion of aa 146-149. Over aa 166-299, human CRX shares 97% aa identity with mouse CRX.

Long Name
Cone-rod Homeobox Protein
Entrez Gene IDs
1406 (Human); 12951 (Mouse); 60446 (Rat)
Alternate Names
cone-rod homeobox; CORD2; CRDcone-rod homeobox protein; CRX; LCA7; LCA7orthodenticle homeobox 3; OTX3

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Citations for Human CRX Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. Patient-specific induced pluripotent stem cells to evaluate the pathophysiology of TRNT1-associated Retinitis pigmentosa
    Authors: TP Sharma, LA Wiley, SS Whitmore, KR Anfinson, CM Cranston, DJ Oppedal, HT Daggett, RF Mullins, BA Tucker, EM Stone
    Stem Cell Res, 2017;21(0):58-70.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  2. cGMP production of patient-specific iPSCs and photoreceptor precursor cells to treat retinal degenerative blindness
    Sci Rep, 2016;6(0):30742.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr


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