Key Product Details

Species Reactivity

Human

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all CSRP2 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human CSRP2
Met1-Gln193
Accession # Q16527

Specificity

Detects human CSRP2 in Western blots.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human CSRP2 Antibody

Detection of Human CSRP2 antibody by Western Blot.

Detection of Human CSRP2 by Western Blot.

Western blot shows lysates of ME-180 human cervical epithelial carcinoma cell line and HeLa human cervical epithelial carcinoma cell line. PVDF Membrane was probed with 2 µg/mL of Goat Anti-Human CSRP2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6196) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for CSRP2 at approximately 21 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Human CSRP2 Antibody

Application
Recommended Usage

Western Blot

2 µg/mL
Sample: ME‑180 human cervical epithelial carcinoma cell line and HeLa human cervical epithelial carcinoma cell line

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: CSRP2

CSRP2 (Cysteine‑rich protein 2/CRP2; also Cysteine and Glycine‑rich protein 2, LMO‑5 and SmLIM) is a 21‑22 kDa member of the CRP family of LIM domain‑containing proteins. It is expressed in arterial smooth muscle, hepatic stellate cells and fibroblasts. CSRP2 is up‑regulated by TGF‑ beta and down‑regulated by PDGF, and appears to interact with PIAS1 and CRP2‑BP, thus promoting STAT signaling. It also binds to SRF and GATA4 to initiate smooth muscle differentiation. Human CSRP2 is 193 amino acids (aa) in length and contains two 50 aa LIM (for lin11/isl1/mec3) domains that contain two zinc‑finger motifs (aa 10‑61 and 119‑170), an NLS between aa 64‑69, and a Gly‑rich C‑terminus (aa 176‑187). Full‑length human CSRP2 shares 79% aa identity with human CSRP1.

Long Name

Cysteine-rich Protein 2

Alternate Names

CRP2, SmLIM

Entrez Gene IDs

1466 (Human); 13008 (Mouse); 29317 (Rat)

Gene Symbol

CSRP2

UniProt

Q16527 (Human)

Additional CSRP2 Products

Product Documents for Human CSRP2 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human CSRP2 Antibody

For research use only

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