Detection of Human CSRP2 by Western Blot.
Western blot shows lysates of ME‑180 human cervical epithelial carcinoma cell line and HeLa human cervical epithelial carcinoma cell line. PVDF Membrane was probed with 2 µg/mL of Goat Anti-Human CSRP2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6196) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for CSRP2 at approximately 21 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
CSRP2 (Cysteine‑rich protein 2/CRP2; also Cysteine and Glycine‑rich protein 2, LMO‑5 and SmLIM) is a 21‑22 kDa member of the CRP family of LIM domain‑containing proteins. It is expressed in arterial smooth muscle, hepatic stellate cells and fibroblasts. CSRP2 is up‑regulated by TGF‑ beta and down‑regulated by PDGF, and appears to interact with PIAS1 and CRP2‑BP, thus promoting STAT signaling. It also binds to SRF and GATA4 to initiate smooth muscle differentiation. Human CSRP2 is 193 amino acids (aa) in length and contains two 50 aa LIM (for lin11/isl1/mec3) domains that contain two zinc‑finger motifs (aa 10‑61 and 119‑170), an NLS between aa 64‑69, and a Gly‑rich C‑terminus (aa 176‑187). Full‑length human CSRP2 shares 79% aa identity with human CSRP1.
Cysteine-rich Protein 2
Entrez Gene IDs:
1466 (Human); 13008 (Mouse); 29317 (Rat)
CRP2; CRP2LIM domain only protein 5; CSRP2; cysteine and glycine-rich protein 2; Cysteine-rich protein 2; LMO5LMO-5; SmLIM; SmLIMLIM domain only 5, smooth muscle; Smooth muscle cell LIM protein
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