Human CX3CL1/Fractalkine Quantikine ELISA Kit

(11 citations)
(1 Review)
  • Assay Type
    Solid Phase Sandwich ELISA
  • Format
    96-well strip plate
  • Assay Length
    4.5 hours
  • Sample Type & Volume Required Per Well
    Cell Culture Supernates (100 uL), Serum (100 uL), EDTA Plasma (100 uL), Heparin Plasma (100 uL), Saliva (50 uL), Urine (50 uL), Human Milk (100 uL)
  • Sensitivity
    0.072 ng/mL
  • Assay Range
    0.2 - 10 ng/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Saliva, Urine, Human Milk)
  • Specificity
    Natural and recombinant human Fractalkine. This assay detects all forms of human Fractalkine containing the chemokine domain.
  • Cross-reactivity
    < 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
  • Interference
    No significant interference observed with available related molecules.
Control Available
Product Summary
The Quantikine Human Fractalkine immunoassay is a 4.5 hour solid phase ELISA designed to measure Fractalkine in cell culture supernates, serum, plasma, saliva, urine, and human milk. It contains NS0-expressed recombinant human Fractalkine and has been shown to accurately quantitate the recombinant factor. Results obtained using natural Fractalkine showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring human Fractalkine.

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Serum, EDTA Plasma, Heparin Plasma, Urine, Human Milk
Intra-Assay Precision Inter-Assay Precision
Standard Deviation0.

Cell Culture Supernates, Saliva
Intra-Assay Precision Inter-Assay Precision
Standard Deviation0.


The recovery of Fractalkine spiked to three different levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Media (n=4) 98 93-102
EDTA Plasma (n=4) 101 88-113
Heparin Plasma (n=4) 97 85-108
Serum (n=4) 99 88-111
Urine (n=4) 96 86-109
To assess the linearity of the assay, samples containing or spiked with high concentrations of Fractalkine were diluted with the appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Human CX3CL1/Fractalkine Quantikine ELISA Kit
Preparation and Storage
  • Storage
    Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: CX3CL1/Fractalkine
CX3CL1/Fractalkine is a transmembrane adhesion protein with a chemokine domain separated from the membrane by a mucin stalk. It is upregulated on many cell types during inflammation. The chemokine and mucin regions can be shed as a soluble chemokine that signals through the receptor CX3CR1. During extravasation, membrane-bound CX3CL1 traps leukocytes, then is cleaved to allow diapedesis. Soluble CX3CL1 protects neurons from microglial neurotoxicity, recruits macrophages for wound healing, recruits osteoclast precursors for bone resorption, and contributes to pathogenesis in coronary artery disease.
    • Entrez Gene IDs
      6376 (Human); 20312 (Mouse); 89808 (Rat);
    • Alternate Names
      ABCD-3; C3Xkine; chemokine (C-X3-C motif) ligand 1; CX3C membrane-anchored chemokine; CXC3; CXC3C; FKN; Fractalkine; Neurotactin; neurotactin); NTNsmall inducible cytokine subfamily D (Cys-X3-Cys), member 1 (fractalkine; NTTSmall-inducible cytokine D1; SCYD1C-X3-C motif chemokine 1; small inducible cytokine subfamily D (Cys-X3-Cys), member-1;
    Related Research Areas
    Assay Procedure
    Refer to the product for complete assay procedure.

    The conjugate must be kept cold during use. Bring all other reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
    1.   Prepare all reagents, standard dilutions, and samples as directed in the product insert.
    2.   Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.

    3. 100 µL Assay Diluent
    4.   Add 100 µL of Assay Diluent to each well.

    5. 100 µL Standard, Control, or Sample
    6.   Add 100 µL of Standard, control, or sample to each well within 15 minutes. Cover with a plate sealer, and incubate at 2-8 °C for 3 hours.
    7.   Aspirate each well and wash, repeating the process 3 times for a total of 4 washes.

    8. 200 µL cold Conjugate
    9.   Add 200 µL of cold Conjugate to each well. Cover with a new plate sealer, and incubate at 2-8 °C for 1 hour.
    10.   Aspirate and wash 4 times.

    11. 200 µL Substrate Solution
    12.   Add 200 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes. PROTECT FROM LIGHT.

    13. 50 µL Stop Solution
    14.   Add 50 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.

    R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

    Showing Results 1 - 10 of 11
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    Sample Type
    1. T lymphocytes and fractalkine contribute to myocardial ischemia/reperfusion injury in patients.
      Authors: Boag S, Das R, Shmeleva E, Bagnall A, Egred M, Howard N, Bennaceur K, Zaman A, Keavney B, Spyridopoulos I
      J Clin Invest, 2015;125(8):3063-76.
      Species: Human
      Sample Type: Serum
    2. Fractalkine is an independent predictor of mortality in patients with advanced heart failure.
      Thromb Haemost, 2012;108(6):1220-7.
      Species: Human
      Sample Type: Plasma
    3. Identification of the chemokine CX3CL1 as a new regulator of malignant cell proliferation in epithelial ovarian cancer.
      Authors: Gaudin F, Nasreddine S, Donnadieu AC, Emilie D, Combadiere C, Prevot S, Machelon V, Balabanian K
      PLoS ONE, 2012;6(7):e21546.
      Species: Human
      Sample Type: Cell Culture Supernates
    4. Activated innate immunity and the involvement of CX3CR1-fractalkine in promoting hematuria in patients with IgA nephropathy.
      Kidney Int., 2012;82(5):548-60.
      Species: Human
      Sample Type: Urine
    5. Altered expression of CX3CL1 in patients with epilepsy and in a rat model.
      Authors: Xu Y, Zeng K, Han Y, Wang L, Chen D, Xi Z, Wang H, Wang X, Chen G
      Am. J. Pathol., 2012;180(5):1950-62.
      Species: Human
      Sample Type: CSF
    6. Fractalkine is expressed in early and advanced atherosclerotic lesions and supports monocyte recruitment via CX3CR1.
      Authors: Stolla M, Pelisek J, von Bruhl M, Schafer A, Barocke V, Heider P, Lorenz M, Tirniceriu A, Steinhart A, Bauersachs J, Bray P, Massberg S, Schulz C
      PLoS ONE, 2012;7(8):e43572.
      Species: Human
      Sample Type: Serum
    7. Systemic biomarkers of inflammation and haemostasis in patients with chronic necrotizing pulmonary aspergillosis.
      BMC Infect Dis, 2012;12(0):144.
      Species: Human
      Sample Type: Serum
    8. Early detection of malignant pleural mesothelioma in asbestos-exposed individuals with a noninvasive proteomics-based surveillance tool.
      Authors: Ostroff R, Mehan M, Stewart A, Ayers D, Brody E, Williams S, Levin S, Black B, Harbut M, Carbone M, Goparaju C, Pass H
      PLoS ONE, 2012;7(10):e46091.
      Species: Human
      Sample Type: Serum
    9. Fractalkine is a novel human adipochemokine associated with type 2 diabetes.
      Authors: Shah R, Hinkle CC, Ferguson JF, Mehta NN, Li M, Qu L, Lu Y, Putt ME, Ahima RS, Reilly MP
      Diabetes, 2011;60(5):1512-8.
      Species: Human
      Sample Type: Plasma
    10. Characterization of synergistic induction of CX3CL1/fractalkine by TNF-alpha and IFN-gamma in vascular endothelial cells: an essential role for TNF-alpha in post-transcriptional regulation of CX3CL1.
      Authors: Matsumiya T, Ota K, Imaizumi T
      J. Immunol., 2010;184(8):4205-14.
      Species: Human
      Sample Type: Cell Culture Supernates
    11. Constitutive endocytosis of the chemokine CX3CL1 prevents its degradation by cell surface metalloproteases.
      Authors: Huang YW, Su P, Liu GY, Crow MR, Chaukos D, Yan H, Robinson LA
      J. Biol. Chem., 2009;284(43):29644-53.
      Species: Human
      Sample Type: Cell Culture Supernates
    Expand to show all
    ELISA Controls
    Description Application Cat# Citations Images  

    Quantikine Immunoassay Control Set 577 Human Fractalkine

    Ctrl QC93
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    Supplemental ELISA Products
    Description Application Cat# Citations Images  

    Quantikine Wash Buffer 1

    ELISA WA126 5
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    Average Rating: 5 (Based on 1 review)

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    Images Ratings Applications Species Reviewed By Date Details
     Human CX3CL1/Fractalkine Quantikine ELISA Kit DCX310
      Anonymous 10/14/2016
    Image Details
     Human CX3CL1/Fractalkine Quantikine ELISA Kit DCX310
    : Human CX3CL1/Fractalkine Quantikine ELISA Kit [DCX310]


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