CX3CL1/Fractalkine is a transmembrane adhesion protein with a chemokine domain separated from the membrane by a mucin stalk. It is upregulated on many cell types during inflammation. The chemokine and mucin regions can be shed as a soluble chemokine that signals through the receptor CX3CR1. During extravasation, membrane-bound CX3CL1 traps leukocytes, then is cleaved to allow diapedesis. Soluble CX3CL1 protects neurons from microglial neurotoxicity, recruits macrophages for wound healing, recruits osteoclast precursors for bone resorption, and contributes to pathogenesis in coronary artery disease.
Human CX3CL1/Fractalkine Quantikine ELISA Kit
R&D Systems | Catalog # DCX310
Loading...
Key Product Details
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (100 µL), Serum (100 µL), EDTA Plasma (100 µL), Heparin Plasma (100 µL), Saliva (50 µL), Urine (50 µL), Human Milk (100 µL)
Sensitivity
0.072 ng/mL
Assay Range
0.156-10 ng/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Saliva, Urine, Human Milk)
Loading...
Product Summary for Human CX3CL1/Fractalkine Quantikine ELISA Kit
The Quantikine Human Fractalkine immunoassay is a 4.5 hour solid phase ELISA designed to measure Fractalkine in cell culture supernates, serum, plasma, saliva, urine, and human milk. It contains NS0-expressed recombinant human Fractalkine and has been shown to accurately quantitate the recombinant factor. Results obtained using natural Fractalkine showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring human Fractalkine.
Product Specifications
Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Measurement
Quantitative ELISA
Detection Method
Colorimetric - 450nm (TMB)
Conjugate
HRP
Species
Human
Specificity
Natural and recombinant human Fractalkine. This assay detects all forms of human Fractalkine containing the chemokine domain.
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates, Saliva
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 40 | 40 | 40 |
| Mean (pg/mL) | 1.18 | 3.33 | 6.43 | 1.19 | 3.53 | 6.85 |
| Standard Deviation | 0.03 | 0.11 | 0.24 | 0.07 | 0.15 | 0.31 |
| CV% | 2.5 | 3.4 | 3.7 | 6.1 | 4.3 | 4.5 |
EDTA Plasma, Heparin Plasma, Human Milk, Serum, Urine
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 40 | 40 | 40 |
| Mean (pg/mL) | 1.24 | 3.80 | 7.12 | 1.23 | 3.59 | 6.88 |
| Standard Deviation | 0.04 | 0.08 | 0.17 | 0.11 | 0.20 | 0.37 |
| CV% | 3.2 | 2.1 | 2.4 | 8.9 | 5.6 | 5.4 |
Recovery for Human CX3CL1/Fractalkine Quantikine ELISA Kit
The recovery of Fractalkine spiked to three different levels throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Media (n=4) | 98 | 93-102 |
| EDTA Plasma (n=4) | 101 | 88-113 |
| Heparin Plasma (n=4) | 97 | 85-108 |
| Serum (n=4) | 99 | 88-111 |
| Urine (n=4) | 96 | 86-109 |
Linearity
To assess the linearity of the assay, samples containing or spiked with high concentrations of Fractalkine were diluted with the appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Scientific Data Images for Human CX3CL1/Fractalkine Quantikine ELISA Kit
Human CX3CL1/Fractalkine ELISA Cell Culture Supernate/Saliva Standard Curve
Human CX3CL1/Fractalkine ELISA Serum/Plasma/Urine/Human Milk Standard Curve
Preparation and Storage
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: CX3CL1/Fractalkine
Alternate Names
FKN, Fractalkine, Neurotactin
Gene Symbol
CX3CL1
Additional CX3CL1/Fractalkine Products
Product Documents for Human CX3CL1/Fractalkine Quantikine ELISA Kit
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Human CX3CL1/Fractalkine Quantikine ELISA Kit
For research use only
⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.Related Research Areas
Citations for Human CX3CL1/Fractalkine Quantikine ELISA Kit
Powered by Bioz
Customer Reviews for Human CX3CL1/Fractalkine Quantikine ELISA Kit (4)
4.8 out of 5
4 Customer Ratings
Have you used Human CX3CL1/Fractalkine Quantikine ELISA Kit?
Submit a review and receive an Amazon gift card!
$25/€18/£15/$25CAN/¥2500 Yen for a review with an image
$10/€7/£6/$10CAN/¥1110 Yen for a review without an image
Submit a review
Customer Images
Showing
1
-
4 of
4 reviews
Showing All
Filter By:
-
Sample Tested: SerumVerified Customer | Posted 11/07/2022
-
Sample Tested: 7TD1 mouse hybridoma cell lineVerified Customer | Posted 07/12/2018
-
Sample Tested: H69AR human small cell lung cancer cell lineVerified Customer | Posted 04/19/2018
-
Sample Tested: Cell Culture SupernatesVerified Customer | Posted 10/14/2016
There are no reviews that match your criteria.
Protocols
View specific protocols for Human CX3CL1/Fractalkine Quantikine ELISA Kit (DCX310):
Refer to the product for complete assay procedure.
The conjugate must be kept cold during use. Bring all other reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
- Prepare all reagents, standard dilutions, and samples as directed in the product insert.
- Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.
- Add 100 µL of Assay Diluent to each well.
- Add 100 µL of Standard, control, or sample to each well within 15 minutes. Cover with a plate sealer, and incubate at 2-8 °C for 3 hours.
- Aspirate each well and wash, repeating the process 3 times for a total of 4 washes.
- Add 200 µL of cold Conjugate to each well. Cover with a new plate sealer, and incubate at 2-8 °C for 1 hour.
- Aspirate and wash 4 times.
- Add 200 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes. PROTECT FROM LIGHT.
- Add 50 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.
100 µL Assay Diluent
100 µL Standard, Control, or Sample
200 µL cold Conjugate
200 µL Substrate Solution
50 µL Stop Solution
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- ELISA Sample Preparation & Collection Guide
- ELISA Troubleshooting Guide
- How to Run an R&D Systems DuoSet ELISA
- How to Run an R&D Systems Quantikine ELISA
- How to Run an R&D Systems Quantikine™ QuicKit™ ELISA
- Quantikine HS ELISA Kit Assay Principle, Alkaline Phosphatase
- Quantikine HS ELISA Kit Principle, Streptavidin-HRP Polymer
- Sandwich ELISA (Colorimetric) – Biotin/Streptavidin Detection Protocol
- Sandwich ELISA (Colorimetric) – Direct Detection Protocol
- Troubleshooting Guide: ELISA
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
Loading...
Associated Pathways
Chemokine Superfamily Pathway: Human/Mouse Ligand-Receptor Interactions
