Human CX3CR1 Fluorescein-conjugated Antibody

Newer Version Available: FAB5204P

Discontinued Product

FAB5204F has been discontinued and is replaced by FAB5204P. FAB5204C.

Detection of CX3CR1 in Human Blood Monocytes by Flow Cytometry.
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Product Details
Citations (1)

Human CX3CR1 Fluorescein-conjugated Antibody Summary

Species Reactivity
Detects human CX3CR1 in direct ELISAs.
Monoclonal Mouse IgG1 Clone # 528728
Protein A or G purified from hybridoma culture supernatant
E. coli-derived recombinant human CX3CR1
Met1-Thr31, Leu91-Lys103, Thr168-Thr195, Lys257-Leu273
Accession # NP_001328
Supplied in a saline solution containing BSA and Sodium Azide.
Fluorescein (Excitation= 488 nm, Emission= 515-545 nm)


Recommended Concentration
Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of CX3CR1 antibody in Human Blood Monocytes antibody by Flow Cytometry. View Larger

Detection of CX3CR1 in Human Blood Monocytes by Flow Cytometry. Human peripheral blood monocytes were stained with Mouse Anti-Human CX3CR1 Fluorescein-conjugated Monoclonal Antibody (Catalog # FAB5204F, filled histogram) or isotype control antibody (Catalog # IC002F, open histogram). View our protocol for Staining Membrane-associated Proteins.

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Preparation and Storage

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: CX3CR1

CX3CR1 is a 40 kDa seven transmembrane chemokine receptor that is expressed on several T cell subsets, monocytes, macrophages, microglia, and epithelial cells. CX3CR1 binding to membrane bound or soluble CX3CR1/Fractalkine promotes inflammatory responses by inducing monocyte adhesion to endothelial cells and macrophage activation. CX3CR1 polymorphisms are associated with the development of chronic inflammatory disorders. Alternately spliced isoforms have extended N-terminal extracellular regions that increase the potency of CX3CR1 as a fusion co-receptor for HIV-1. Human CX3CR1 shares 82% amino acid sequence identity with mouse and rat CX3CR1.

Long Name
CX3C Chemokine Receptor 1
Entrez Gene IDs
1524 (Human); 13051 (Mouse); 171056 (Rat)
Alternate Names
Beta chemokine receptor-like 1; CCRL1; chemokine (C-C) receptor-like 1; chemokine (C-X3-C motif) receptor 1; chemokine (C-X3-C) receptor 1; CMKbRL1; CMK-BRL1; CMK-BRL-1; CMKDR1; CX3C chemokine receptor 1; CX3CR1; Fractalkine receptor; G protein-coupled receptor 13; GPR13G-protein coupled receptor 13; GPRV28; V28; V28C-X3-C CKR-1

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Citation for Human CX3CR1 Fluorescein-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. CX3CL1/CX3CR1 and CCL2/CCR2 Chemokine/Chemokine Receptor Complex in Patients with AMD.
    Authors: Falk M, Singh A, Faber C, Nissen M, Hviid T, Sorensen T
    PLoS ONE, 2014;9(12):e112473.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry


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