Human CXCL8/IL-8 Quantikine ELISA Kit

(194 citations)   
  • Assay Type
    Solid Phase Sandwich ELISA
  • Format
    96-well strip plate
  • Assay Length
    3.5 hours
  • Sample Type & Volume Required Per Well
    Cell Culture Supernates (50 uL), Serum (50 uL), EDTA Plasma (50 uL), Heparin Plasma (50 uL), Citrate Plasma (50 uL)
  • Sensitivity
    7.5 pg/mL
  • Assay Range
    31.2 - 2,000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Citrate Plasma)
  • Specificity
    Natural and recombinant human IL-8
  • Cross-reactivity
    < 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
  • Interference
    No significant interference observed with available related molecules.
Control Available
Product Summary
The Quantikine Human IL-8 Immunoassay is a 3.5 hour solid phase ELISA designed to measure human IL-8 in cell culture supernates, serum, and plasma. It is based on antibodies raised against the 72 aa variant of human IL-8 derived from E. coli. It is calibrated with the same recombinant factor. This immunoassay accurately quantitates recombinant human IL-8. Measurement of natural human IL-8 or the 77 aa variant of human IL-8 gave results parallel to the standard curves obtained using the E. coli-expressed Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural human IL-8.

Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) hree samples of known concentration were tested in separate assays to assess inter-assay precision.
Serum, EDTA Plasma, Heparin Plasma, Citrate Plasma
Intra-Assay Precision Inter-Assay Precision
Sample123123
n202020202020
Mean16852610931965811160
Standard Deviation9.428.670.714.556.670.2
CV%5.65.46.57.49.76.1

Cell Culture Supernates
Intra-Assay Precision Inter-Assay Precision
Sample123123
n202020202020
Mean115386802132410817
Standard Deviation5.31737.910.72842.4
CV%4.64.44.78.16.85.2

Recovery

The recovery of IL-8 spiked to three different levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Media (n=5) 98 85-114
Citrate Plasma (n=5) 105 95-114
EDTA Plasma (n=5) 103 97-111
Heparin Plasma (n=5) 102 92-107
Serum (n=5) 98 88-106
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of IL-8 in various matrices and diluted with the appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Human CXCL8/IL-8 Quantikine ELISA Kit
Preparation and Storage
  • Storage
    Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: CXCL8/IL-8
CXCL8/Interleukin-8 is a chemokine that is upregulated at sites of inflammation where it promotes neutrophil infiltration and activation. CXCL8 can form homodimers and heterodimers with CXCL4/PF4. Its bioactivity is regulated by proteolytic truncations, citrullination, and the decoy receptor DARC. CXCL8 signals through CXCR1/IL-8 RA, which is also used by CXCL6, and through CXCR2/IL-8 RB, which is used by multiple CXC chemokines. CXCL8 also binds to Serpin A1/alpha-1 Antitrypsin which prevents CXCL8 interaction with CXCR1.
    • Entrez Gene IDs
      3576 (Human); 396880 (Porcine); 403850 (Canine); 493836 (Feline);
    • Alternate Names
      3-10C; AMCF-I; C-X-C motif chemokine 8; CXCL8SCYB8; Emoctakin; GCP1; GCP-1TSG-1; IL8; interleukin 8; K60; LAI; LECT; MDNCF; MDNCFb-ENAP; member 8; MONAPGCP1; NAP1; NAP-1NAP1; NCF; Neutrophil-activating protein 1; Protein 3-10C; T cell chemotactic factor; T-cell chemotactic factor; TCF; TSG1;
    Related Research Areas
    Assay Procedure
    Refer to the product for complete assay procedure.

    Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
    1.   Prepare all reagents, standard dilutions, and samples as directed in the product insert.
    2.   Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.

    3. 100 µL Assay Diluent
    4.   Add 100 µL of Assay Diluent to each well.

    5. 50 µL Standard, Control, or Sample
    6.   Add 50 µL of Standard, control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours.
    7.   Aspirate each well and wash, repeating the process 3 times for a total of 4 washes.

    8. 100 µL Conjugate
    9.   Add 100 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 1 hour.
    10.   Aspirate and wash 4 times.

    11. 200 µL Substrate Solution
    12.   Add 200 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes. PROTECT FROM LIGHT.

    13. 50 µL Stop Solution
    14.   Add 50 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.
    Citations:

    R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

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    Species
    Sample Type
    1. Association between visceral fat, IL-8 and endometrial cancer.
      Authors: Ciortea, Razvan, Mihu, Dan, Mihu, Carmen M
      Anticancer Res, 2014;34(1):379-83.
      Species: Human
      Sample Type: Plasma
    2. Thrombin-induced CCAAT/enhancer-binding protein beta activation and IL-8/CXCL8 expression via MEKK1, ERK, and p90 ribosomal S6 kinase 1 in lung epithelial cells.
      Authors: Lin C, Nai P, Bien M, Yu C, Chen B
      J Immunol, 2014;192(1):338-48.
      Species: Human
      Sample Type: Cell Culture Supernates
    3. Mycobacterium bovis bacilli Calmette-Guerin regulates leukocyte recruitment by modulating alveolar inflammatory responses.
      Authors: Andersson M, Lutay N, Hallgren O, Westergren-Thorsson G, Svensson M, Godaly G
      Innate Immun, 2012;18(3):531-40.
      Species: Human
      Sample Type: Cell Culture Supernates
    4. Late SV40 factor (LSF) enhances angiogenesis by transcriptionally up-regulating matrix metalloproteinase-9 (MMP-9).
      Authors: Santhekadur PK, Gredler R, Chen D
      J. Biol. Chem., 2012;287(5):3425-32.
      Species: Human
      Sample Type: Cell Culture Supernates
    5. TLR4 Protein Contributes to Cigarette Smoke-induced Matrix Metalloproteinase-1 (MMP-1) Expression in Chronic Obstructive Pulmonary Disease.
      Authors: Geraghty P, Dabo AJ, D'Armiento J
      J. Biol. Chem., 2011;286(34):30211-8.
      Species: Human
      Sample Type: Cell Culture Supernates
    6. Mannitol challenge for assessment of airway responsiveness, airway inflammation and inflammatory phenotype in asthma.
      Authors: Wood LG, Powell H, Gibson PG
      Clin. Exp. Allergy, 2010;40(2):232-41.
      Species: Human
      Sample Type: Saliva
    7. Discovery of a novel class of targeted kinase inhibitors that blocks protein kinase C signaling and ameliorates retinal vascular leakage in a diabetic rat model.
      Authors: Grant S, Tran P, Zhang Q, Zou A, Dinh D, Jensen J, Zhou S, Kang X, Zachwieja J, Lippincott J, Liu K, Johnson SL, Scales S, Yin C, Nukui S, Stoner C, Prasanna G, Lafontaine J, Wells P, Li H
      Eur. J. Pharmacol., 2010;627(1):16-25.
      Species: Human
      Sample Type: Cell Culture Supernates
    8. Androgen-regulated expression of arginase 1, arginase 2 and interleukin-8 in human prostate cancer.
      Authors: Gannon PO, Godin-Ethier J, Hassler M, Delvoye N, Aversa M, Poisson AO, Peant B, Alam Fahmy M, Saad F, Lapointe R, Mes-Masson AM
      PLoS ONE, 2010;5(8):e12107.
      Species: Human
      Sample Type: Cell Culture Supernates
    9. Immunomodulation of nasal epithelial cells by Staphylococcus aureus-derived serine proteases.
      Authors: Rudack C, Sachse F, Albert N, Becker K, von Eiff C
      J. Immunol., 2009;183(11):7592-601.
      Species: Human
      Sample Type: Cell Culture Supernates
    10. Uropathogenic Escherichia coli modulates immune responses and its curli fimbriae interact with the antimicrobial peptide LL-37.
      Authors: Kai-Larsen Y, Luthje P, Chromek M, Peters V, Wang X, Holm A, Kadas L, Hedlund KO, Johansson J, Chapman MR, Jacobson SH, Romling U, Agerberth B, Brauner A
      PLoS Pathog., 2010;6(0):e1001010.
      Species: Human
      Sample Type: Urine
    11. Disordered macrophage cytokine secretion underlies impaired acute inflammation and bacterial clearance in Crohn's disease.
      Authors: Smith AM, Rahman FZ, Hayee B, Graham SJ, Marks DJ, Sewell GW, Palmer CD, Wilde J, Foxwell BM, Gloger IS, Sweeting T, Marsh M, Walker AP, Bloom SL, Segal AW
      J. Exp. Med., 2009;206(9):1883-97.
      Species: Human
      Sample Type: Cell Culture Supernates
    12. Role of inflammation and insulin resistance in endothelial progenitor cell dysfunction.
      Authors: Desouza CV, Hamel FG, Bidasee K, O'Connell K
      Diabetes, 2011;60(4):1286-94.
      Species: Human
      Sample Type: Cell Culture Supernates
    13. Soluble TLR2 is present in human amniotic fluid and modulates the intraamniotic inflammatory response to infection.
      Authors: Dulay AT, Buhimschi CS, Zhao G, Oliver EA, Mbele A, Jing S, Buhimschi IA
      J. Immunol., 2009;182(11):7244-53.
      Species: Human
      Sample Type: Amniotic Fluid
    14. Crimean Congo hemorrhagic fever virus infects human monocyte-derived dendritic cells.
      Authors: Connolly-Andersen AM, Douagi I, Kraus AA, Mirazimi A
      Virology, 2009;390(2):157-62.
      Species: Human
      Sample Type: Cell Culture Supernates
    15. Dysregulation of stromal derived factor 1/CXCR4 axis in the megakaryocytic lineage in essential thrombocythemia.
      Authors: Salim JP, Goette NP, Lev PR, Chazarreta CD, Heller PG, Alvarez C, Molinas FC, Marta RF
      Br. J. Haematol., 2009;144(1):69-77.
      Species: Human
      Sample Type: Plasma
    16. Particulate matter in cigarette smoke alters iron homeostasis to produce a biological effect.
      Authors: Ghio AJ, Hilborn ED, Stonehuerner JG, Dailey LA, Carter JD, Richards JH, Crissman KM, Foronjy RF, Uyeminami DL, Pinkerton KE
      Am. J. Respir. Crit. Care Med., 2008;178(11):1130-8.
      Species: Human
      Sample Type: BALF
    17. 1, 25 Dihydroxyvitamin D3 modulated cytokine response in pulmonary tuberculosis.
      Authors: Vidyarani M, Selvaraj P, Jawahar MS, Narayanan PR
      Cytokine, 2007;40(2):128-34.
      Species: Human
      Sample Type: Cell Culture Supernates
    18. Securinine, a GABAA receptor antagonist, enhances macrophage clearance of phase II C. burnetii: comparison with TLR agonists.
      Authors: Lubick K, Radke M, Jutila M
      J. Leukoc. Biol., 2007;82(5):1062-9.
      Species: Human
      Sample Type: Cell Culture Supernates
    19. Clarithromycin targets neutrophilic airway inflammation in refractory asthma.
      Authors: Simpson JL, Powell H, Boyle MJ, Scott RJ, Gibson PG
      Am. J. Respir. Crit. Care Med., 2007;177(2):148-55.
      Species: Human
      Sample Type: Sputum
    20. Serum and urine levels of interleukin-8 in patients with non-Hodgkin's lymphoma.
      Authors: Lee HL, Eom HS, Yun T, Kim HJ, Park WS, Nam BH, Moon-Woo S, Lee DH, Kong SY
      Cytokine, 2008;43(1):71-5.
      Species: Human
      Sample Type: Serum
    21. Increased levels of LPS-binding protein in bovine blood and milk following bacterial lipopolysaccharide challenge.
      Authors: Bannerman DD, Paape MJ, Hare WR, Sohn EJ
      J. Dairy Sci., 2003;86(10):3128-37.
      Species: Bovine
      Sample Type: Milk
    22. Complement fragment C5a and inflammatory cytokines in neutrophil recruitment during intramammary infection with Escherichia coli.
      Authors: Shuster DE, Kehrli ME, Rainard P, Paape M
      Infect. Immun., 1997;65(8):3286-92.
      Species: Bovine
      Sample Type: Serum
    23. Specificity of immunomodulator secretion in urinary samples in response to infection by alpha-hemolysin and CNF1 bearing uropathogenic Escherichia coli.
      Authors: Real JM, Munro P, Buisson-Touati C, Lemichez E, Boquet P, Landraud L
      Cytokine, 2007;37(1):22-5.
      Species: Human
      Sample Type: Urine
    24. MMP-12 induces IL-8/CXCL8 secretion through EGFR and ERK1/2 activation in epithelial cells.
      Authors: Le Quement C, Guenon I, Gillon JY, Lagente V, Boichot E
      Am. J. Physiol. Lung Cell Mol. Physiol., 2008;294(6):L1076-84.
      Species: Human
      Sample Type: Cell Culture Supernates
    25. Innate immune activation in neutrophilic asthma and bronchiectasis.
      Authors: Simpson JL, Grissell TV, Douwes J, Scott RJ, Boyle MJ, Gibson PG
      Thorax, 2007;62(3):211-8.
      Species: Human
      Sample Type: Sputum
    26. MAPK pathway mediates EGR-1-HSP70-dependent cigarette smoke-induced chemokine production.
      Authors: Li CJ, Ning W, Matthay MA, Feghali-Bostwick CA, Choi AM
      Am. J. Physiol. Lung Cell Mol. Physiol., 2007;292(5):L1297-303.
      Species: Human
      Sample Type: Cell Culture Supernates
    27. Pathogenesis of Ebola hemorrhagic fever in cynomolgus macaques: evidence that dendritic cells are early and sustained targets of infection.
      Authors: Geisbert TW, Hensley LE, Larsen T, Young HA, Reed DS, Geisbert JB, Scott DP, Kagan E, Jahrling PB, Davis KJ
      Am. J. Pathol., 2003;163(6):2347-70.
      Species: Primate - Macaca fascicularis (Crab-eating or Cynomolgus macaque)
      Sample Type: Serum
    28. Local immunostimulation induced by intravesical administration of autologous interferon-gamma-activated macrophages in patients with superficial bladder cancer.
      Authors: Pages F, Lebel-Binay S, Vieillefond A, Deneux L, Cambillau M, Soubrane O, Debre B, Tardy D, Lemonne JL, Abastado JP, Fridman WH, Thiounn N
      Clin. Exp. Immunol., 2002;127(2):303-9.
      Species: Human
      Sample Type: Urine
    29. Compositional difference in middle ear effusion: mucous versus serous.
      Authors: Chung MH, Choi JY, Lee WS, Kim HN, Yoon JH
      Laryngoscope, 2002;112(1):152-5.
      Species: Human
      Sample Type: middle ear effusion
    30. Hepatocyte growth factor in polymorphonuclear leukocytes is increased in patients with systemic inflammatory response syndrome.
      Authors: Matsushima A, Ogura H, Koh T, Fujita K, Yoshiya K, Sumi Y, Hosotsubo H, Kuwagata Y, Tanaka H, Shimazu T, Sugimoto H
      J Trauma, 2004;56(2):259-64.
      Species: Human
      Sample Type: Plasma
    31. Guiding the vaginal microbicide trials with biomarkers of inflammation.
      Authors: Fichorova RN
      J. Acquir. Immune Defic. Syndr., 2004;37(0):S184-93.
      Species: Human
      Sample Type: cervicovaginal lavage
    32. Central role of complement membrane attack complex in monosodium urate crystal-induced neutrophilic rabbit knee synovitis.
      Authors: Tramontini N, Huber C, Liu-Bryan R, Terkeltaub RA, Kilgore KS
      Arthritis Rheum., 2004;50(8):2633-9.
      Species: Rabbit
      Sample Type: Synovial Fluid
    33. Bronchiectasis, exacerbation indices, and inflammation in chronic obstructive pulmonary disease.
      Authors: Patel IS, Vlahos I, Wilkinson TM, Lloyd-Owen SJ, Donaldson GC, Wilks M, Reznek RH, Wedzicha JA
      Am. J. Respir. Crit. Care Med., 2004;170(4):400-7.
      Species: Human
      Sample Type: Sputum
    34. Embryotoxicity of peritoneal fluid in women with endometriosis. Its relation with cytokines and lymphocyte populations.
      Authors: Gomez-Torres MJ, Acien P, Campos A, Velasco I
      Hum. Reprod., 2002;17(3):777-81.
      Species: Human
      Sample Type: Peritoneal Fluid
    35. Airway antioxidant and inflammatory responses to diesel exhaust exposure in healthy humans.
      Authors: Behndig AF, Mudway IS, Brown JL, Stenfors N, Helleday R, Duggan ST, Wilson SJ, Boman C, Cassee FR, Frew AJ, Kelly FJ, Sandstrom T, Blomberg A
      Eur. Respir. J., 2006;27(2):359-65.
    36. Airway inflammation during stable and acutely exacerbated chronic obstructive pulmonary disease.
      Authors: Fujimoto K, Yasuo M, Urushibata K, Hanaoka M, Koizumi T, Kubo K
      Eur. Respir. J., 2005;25(4):640-6.
      Species: Human
      Sample Type: Sputum
    37. Expression of functional CCR and CXCR chemokine receptors in podocytes.
      Authors: Huber TB, Reinhardt HC, Exner M, Burger JA, Kerjaschki D, Saleem MA, Pavenstadt H
      J. Immunol., 2002;168(12):6244-52.
      Species: Human
      Sample Type: Cell Culture Supernates
    38. Validation of assays for inflammatory mediators in exhaled breath condensate.
      Authors: Bayley DL, Abusriwil H, Ahmad A, Stockley RA
      Eur. Respir. J., 2008;31(5):943-8.
      Species: Human
      Sample Type: Sputum
    39. Cimetidine does not prevent lung injury in newborn premature infants.
      Authors: Cotton RB, Hazinski TA, Morrow JD, Roberts LJ, Zeldin DC, Lindstrom DP, Lappalainen U, Law AB, Steele S
      Pediatr. Res., 2006;59(6):795-800.
      Species: Human
      Sample Type: Tracheal Lavage
    40. The effect of respiratory synctial virus on chemokine release by differentiated airway epithelium.
      Authors: Mellow TE, Murphy PC, Carson JL, Noah TL, Zhang L, Pickles RJ
      Exp. Lung Res., 2004;30(1):43-57.
      Species: Human
      Sample Type: Cell Culture Supernates
    41. Nasal inflammation and personal exposure to fine particles PM2.5 in asthmatic children.
      Authors: Nikasinovic L, Just J, Sahraoui F, Seta N, Grimfeld A, Momas I
      J. Allergy Clin. Immunol., 2006;117(6):1382-8.
      Species: Human
      Sample Type: nasal lavage
    42. Relationship between airway inflammation and the frequency of exacerbations in patients with smoking related COPD.
      Authors: Gompertz S, Bayley DL, Hill SL, Stockley RA
      Thorax, 2001;56(1):36-41.
      Species: Human
      Sample Type: Saliva
    43. Lung injury after ozone exposure is iron dependent.
      Authors: Ghio AJ, Turi JL, Madden MC, Dailey LA, Richards JD, Stonehuerner JG, Morgan DL, Singleton S, Garrick LM, Garrick MD
      Am. J. Physiol. Lung Cell Mol. Physiol., 2007;292(1):L134-43.
      Species: Human
      Sample Type: Cell Culture Supernates
    44. Airway neutrophil inflammation in nonasthmatic patients with food allergy.
      Authors: Wallaert B, Gosset P, Lamblin C, Garcia G, Perez T, Tonnel AB
      Allergy, 2002;57(5):405-10.
      Species: Human
      Sample Type: Saliva
    45. Relationship of changes in interleukin-8 levels and granulocyte elastase activity in gingival crevicular fluid to subgingival periodontopathogens following non-surgical periodontal therapy in subjects with chronic periodontitis.
      Authors: Jin LJ, Leung WK, Corbet EF, Soder B
      J. Clin. Periodontol., 2002;29(7):604-14.
      Species: Human
      Sample Type: Gingival Crevicular Fluid (GCF)
    46. Chlamydia pneumoniae immunoglobulin A reactivation and airway inflammation in acute asthma.
      Authors: Wark PA, Johnston SL, Simpson JL, Hensley MJ, Gibson PG
      Eur. Respir. J., 2002;20(4):834-40.
      Species: Human
      Sample Type: Sputum
    47. Interleukin-8 production in tuberculous pleurisy: role of mesothelial cells stimulated by cytokine network involving tumour necrosis factor-alpha and interleukin-1 beta.
      Authors: Park JS, Kim YS, Jee YK, Myong NH, Lee KY
      Scand. J. Immunol., 2003;57(5):463-9.
      Species: Human
      Sample Type: Pleural Effusion
    48. Reduced inspiratory flow attenuates IL-8 release and MAPK activation of lung overstretch.
      Authors: Kotani M, Kotani T, Li Z, Silbajoris R, Piantadosi CA, Huang YC
      Eur. Respir. J., 2004;24(2):238-46.
      Species: Rabbit
      Sample Type: Tissue Homogenates
    49. Normal nasal mucociliary clearance in CF children: evidence against a CFTR-related defect.
      Authors: McShane D, Davies JC, Wodehouse T, Bush A, Geddes D, Alton EW
      Eur. Respir. J., 2004;24(1):95-100.
      Species: Human
      Sample Type: nasal lavage
    50. NOD1 contributes to mouse host defense against Helicobacter pylori via induction of type I IFN and activation of the ISGF3 signaling pathway.
      Authors: Watanabe T, Asano N, Fichtner-Feigl S, Gorelick PL, Tsuji Y, Matsumoto Y, Chiba T, Fuss IJ, Kitani A, Strober W
      J. Clin. Invest., 2010;120(5):1645-62.
      Species: Human
      Sample Type: Cell Culture Supernates
    51. Multiplex bead array assays for detection of soluble cytokines: comparisons of sensitivity and quantitative values among kits from multiple manufacturers.
      Authors: Khan SS, Smith MS, Reda D, Suffredini AF, McCoy JP
      Cytometry B Clin Cytom, 2004;61(1):35-9.
      Species: Human
      Sample Type: Serum
    52. Influence of eicosapentaenoic acid, an omega-3 fatty acid, on ultraviolet-B generation of prostaglandin-E2 and proinflammatory cytokines interleukin-1 beta, tumor necrosis factor-alpha, interleukin-6 and interleukin-8 in human skin in vivo.
      Authors: Shahbakhti H, Watson RE, Azurdia RM, Ferreira CZ, Garmyn M, Rhodes LE
      Photochem. Photobiol., 2004;80(2):231-5.
      Species: Human
      Sample Type: Plasma
    53. CXCR2 promotes ovarian cancer growth through dysregulated cell cycle, diminished apoptosis, and enhanced angiogenesis.
      Authors: Yang G, Rosen DG, Liu G
      Clin. Cancer Res., 2010;16(15):3875-86.
      Species: Human
      Sample Type: Cell Culture Supernates
    54. Rickettsia rickettsii infection of human macrovascular and microvascular endothelial cells reveals activation of both common and cell type-specific host response mechanisms.
      Authors: Rydkina E, Turpin LC, Sahni SK
      Infect. Immun., 2010;78(6):2599-606.
      Species: Human
      Sample Type: Cell Culture Supernates
    55. Cytokine pattern in blister fluid and sera of patients with pemphigus.
      Authors: Baroni A, Perfetto B, Ruocco E, Greco R, Criscuolo D, Ruocco V
      Dermatology (Basel), 2002;205(2):116-21.
      Species: Human
      Sample Type: blister fluid
    56. IL-33 promotes the migration and proliferation of circulating fibrocytes from patients with allergen-exacerbated asthma.
      Authors: Bianchetti L, Marini M, Isgro M, Bellini A, Schmidt M, Mattoli S
      Biochem Biophys Res Commun, 2012;426(1):116-21.
      Species: Human
      Sample Type: Cell Culture Supernates
    57. Characterization of innate immune signalling receptors in virus-induced acute asthma.
      Authors: Wood LG, Simpson JL, Wark PA, Powell H, Gibson PG
      Clin. Exp. Allergy, 2011;41(5):640-8.
      Species: Human
      Sample Type: Saliva
    58. Melatonin attenuates the TLR4-mediated inflammatory response through MyD88- and TRIF-dependent signaling pathways in an in vivo model of ovarian cancer.
      Authors: Chuffa L, Fioruci-Fontanelli B, Mendes L, Ferreira Seiva F, Martinez M, Favaro W, Domeniconi R, Pinheiro P, Delazari Dos Santos L, Martinez F
      BMC Cancer, 2015;15(0):34.
      Species: Rat
      Sample Type: Tissue Supernate
    59. Function of interleukin-20 as a proinflammatory molecule in rheumatoid and experimental arthritis.
      Authors: Hsu YH, Li HH, Hsieh MY, Liu MF, Huang KY, Chin LS, Chen PC, Cheng HH, Chang MS
      Arthritis Rheum., 2006;54(9):2722-33.
      Species: Human
      Sample Type: Cell Culture Supernates
    60. Analysis of plasma cytokines and angiogenic factors in patients with pretreated urothelial cancer receiving Pazopanib: the role of circulating interleukin-8 to enhance the prognostic accuracy.
      Authors: Necchi A, Pennati M, Zaffaroni N, Landoni E, Giannatempo P, Raggi D, Schwartz L, Morosi C, Crippa F, Fare E, Nicolai N, Lanocita R, Sava T, Sacco C, Messina C, Ortega C, De Braud F, Salvioni R, Daidone M, Gianni A, Mariani L
      Br J Cancer, 2014;110(1):26-33.
      Species: Human
      Sample Type: Plasma
    61. Prevention of F-actin assembly switches the response to SCF from chemotaxis to degranulation in human mast cells.
      Authors: Smrz D, Bandara G, Beaven M, Metcalfe D, Gilfillan A
      Eur J Immunol, 2013;43(7):1873-82.
      Species: Human
      Sample Type: Cell Culture Supernates
    62. High levels and inflammatory effects of soluble CXC ligand 16 (CXCL16) in coronary artery disease: down-regulatory effects of statins.
      Authors: Smith C, Halvorsen B, Otterdal K, Waehre T, Yndestad A, Fevang B, Sandberg WJ, Breland UM, Froland SS, Oie E, Gullestad L, Damas JK, Aukrust P
      Cardiovasc. Res., 2008;79(1):195-203.
      Species: Human
      Sample Type: Cell Culture Supernates
    63. IFN-gamma alters the response of Borrelia burgdorferi-activated endothelium to favor chronic inflammation.
      Authors: Dame TM, Orenzoff BL, Palmer LE, Furie MB
      J. Immunol., 2007;178(2):1172-9.
      Species: Human
      Sample Type: Cell Culture Supernates
    64. CXC-chemokine stimulation of neutrophils correlates with plasma levels of myeloperoxidase and lactoferrin and contributes to clinical outcome after pediatric cardiac surgery.
      Authors: Gessler P, Pretre R, Hohl V, Rousson V, Fischer J, Dahinden C
      Shock, 2004;22(6):513-20.
      Species: Human
      Sample Type: Plasma
    65. High expression of ligands for chemokine receptor CXCR2 in alveolar epithelial neoplasia induced by oncogenic kras.
      Authors: Wislez M, Fujimoto N, Izzo JG, Hanna AE, Cody DD, Langley RR, Tang H, Burdick MD, Sato M, Minna JD, Mao L, Wistuba I, Strieter RM, Kurie JM
      Cancer Res., 2006;66(8):4198-207.
      Species: Human
      Sample Type: Cell Culture Supernates
    66. Cytokine profiling in human colostrum and milk by protein array.
      Authors: Kverka M, Burianova J, Lodinova-Zadnikova R, Kocourkova I, Cinova J, Tuckova L, Tlaskalova-Hogenova H
      Clin. Chem., 2007;53(5):955-62.
      Species: Human
      Sample Type: Milk
    67. Protective mucosal immunity elicited by targeted iliac lymph node immunization with a subunit SIV envelope and core vaccine in macaques.
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      Species: Human
      Sample Type: Tissue Homogenates
    146. Tissue engineering for full-thickness burns: a dermal substitute from bench to bedside.
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      Sample Type: Cell Culture Supernates
    147. Co-occurrence of IgA antibodies against ethanol metabolites and tissue transglutaminase in alcohol consumers: correlation with proinflammatory cytokines and markers of fibrogenesis.
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      Dig. Dis. Sci., 2007;53(2):500-5.
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    148. Human Herpesvirus 8 (HHV8) sequentially shapes the NK cell repertoire during the course of asymptomatic infection and Kaposi sarcoma.
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    149. Functional genomic analysis in arthritis-affected cartilage: yin-yang regulation of inflammatory mediators by alpha 5 beta 1 and alpha V beta 3 integrins.
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    151. Increased expression of p38 MAPK in human bronchial epithelium after lipopolysaccharide exposure.
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      Eur. Respir. J., 2005;25(5):797-803.
    152. Relationships between allergic inflammation and nasal airflow in children with seasonal allergic rhinitis.
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      Ann. Allergy Asthma Immunol., 2005;94(2):258-61.
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    153. Age-dependent mobilization of circulating endothelial progenitor cells in infants and young children undergoing cardiac surgery with cardiopulmonary bypass.
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      Sample Type: Plasma
    154. Spontaneous circulation of myeloid-lymphoid-initiating cells and SCID-repopulating cells in sickle cell crisis.
      Authors: Lamming CE, Augustin L, Blackstad M, Lund TC, Hebbel RP, Verfaillie CM
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      Sample Type: Serum
    155. Inflammatory interaction between LIGHT and proteinase-activated receptor-2 in endothelial cells: potential role in atherogenesis.
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    156. A randomized trial of recombinant human granulocyte-macrophage colony stimulating factor for patients with acute lung injury.
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    157. Interleukin-18 is a strong predictor of cardiovascular events in elderly men with the metabolic syndrome: synergistic effect of inflammation and hyperglycemia.
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    158. Skewing of cytotoxic activity and chemokine production, but not of chemokine receptor expression, in human type-1/-2 gamma delta T lymphocytes.
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    159. Th1/Th2 cytokines reciprocally regulate in vitro pulmonary angiogenesis via CXC chemokine synthesis.
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      Am. J. Respir. Cell Mol. Biol., 2007;38(2):168-75.
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    160. A novel IL-1 family cytokine, IL-33, potently activates human eosinophils.
      Authors: Cherry WB, Yoon J, Bartemes KR, Iijima K, Kita H
      J. Allergy Clin. Immunol., 2008;121(6):1484-90.
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    161. In vitro and in vivo effects of salbutamol on neutrophil function in acute lung injury.
      Authors: Perkins GD, Nathani N, McAuley DF, Gao F, Thickett DR
      Thorax, 2007;62(1):36-42.
      Species: Human
      Sample Type: BALF
    162. Increased NOS2 predicts poor survival in estrogen receptor-negative breast cancer patients.
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      J. Clin. Invest., 2010;120(11):3843-54.
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      Sample Type: Cell Culture Supernates
    163. The influence of anger expression on wound healing.
      Authors: Gouin JP, Kiecolt-Glaser JK, Malarkey WB, Glaser R
      Brain Behav. Immun., 2007;22(5):699-708.
      Species: Human
      Sample Type: Wound Secretion
    164. Urine cytokines profile in renal transplant patients with asymptomatic bacteriuria.
      Authors: Ciszek M, Paczek L, Bartlomiejczyk I, Mucha K
      Transplantation, 2006;81(12):1653-7.
      Species: Human
      Sample Type: Urine
    165. Proliferation, differentiation, and cytokine secretion of human umbilical cord blood-derived mononuclear cells in vitro.
      Authors: Neuhoff S, Moers J, Rieks M, Grunwald T, Jensen A, Dermietzel R, Meier C
      Exp. Hematol., 2007;35(7):1119-31.
      Species: Human
      Sample Type: Cell Culture Supernates
    166. C-reactive protein and vascular cell adhesion molecule-1 as markers of severity in sickle cell disease.
      Authors: Makis AC, Hatzimichael EC, Stebbing J, Bourantas KL
      Arch. Intern. Med., 2006;166(3):366-8.
      Species: Human
      Sample Type: Plasma
    167. The major outer membrane protein of a periodontopathogen induces IFN-beta and IFN-stimulated genes in monocytes via lipid raft and TANK-binding kinase 1/IFN regulatory factor-3.
      Authors: Lee SH, Kim JS, Jun HK
      J. Immunol., 2009;182(9):5823-35.
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    168. Glucocorticoid-induced tumour necrosis factor receptor-related protein-mediated macrophage stimulation may induce cellular adhesion and cytokine expression in rheumatoid arthritis.
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      Clin. Exp. Immunol., 2007;148(3):410-8.
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    169. Protection against inflammation- and autoantibody-caused fetal loss by the chemokine decoy receptor D6.
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    170. IL-8 as antibody therapeutic target in inflammatory diseases: reduction of clinical activity in palmoplantar pustulosis.
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      Species: Human
      Sample Type: palmoplantar pustulosis wash fluid
    171. Role of interleukin-8 and growth-regulated oncogene-alpha in the chemotactic migration of all-trans retinoic acid-treated promyelocytic leukemic cells toward alveolar epithelial cells.
      Authors: Tsai WH, Hsu HC, Lin CC, Ho CK, Kou YR
      Crit. Care Med., 2007;35(3):879-85.
      Species: Human
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    172. In vitro induction of a dendritic cell phenotype in primary human acute myelogenous leukemia (AML) blasts alters the chemokine release profile and increases the levels of T cell chemotactic CCL17 and CCL22.
      Authors: Olsnes AM, Ryningen A, Ersvaer E, Bruserud Ø
      J. Interferon Cytokine Res., 2008;28(5):297-310.
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    173. Different angiogenic activity in pulmonary sarcoidosis and idiopathic pulmonary fibrosis.
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      Chest, 2006;130(4):982-8.
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    175. Computed tomographic scan-diagnosed chronic obstructive pulmonary disease-emphysema: eotaxin-1 is associated with bronchodilator response and extent of emphysema.
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      J. Allergy Clin. Immunol., 2007;120(5):1118-25.
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      Authors: Alexander GM, Perreault MJ, Reichenberger ER, Schwartzman RJ
      Brain Behav. Immun., 2006;21(5):668-76.
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    177. Effect of interactions between lower airway bacterial and rhinoviral infection in exacerbations of COPD.
      Authors: Wilkinson TM, Hurst JR, Perera WR, Wilks M, Donaldson GC, Wedzicha JA
      Chest, 2006;129(2):317-24.
      Species: Human
      Sample Type: Saliva
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      Authors: Yagami A, Orihara K, Morita H
      J. Immunol., 2010;185(10):5743-50.
      Species: Human
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    179. cAMP/PKA pathway activation in human mesenchymal stem cells in vitro results in robust bone formation in vivo.
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      Species: Human
      Sample Type: Cell Culture Supernates
    180. Medium-chain fatty acid-sensing receptor, GPR84, is a proinflammatory receptor.
      Authors: Suzuki M, Takaishi S, Nagasaki M, Onozawa Y, Iino I, Maeda H, Komai T, Oda T
      J Biol Chem, 2013;288(15):10684-91.
      Species: Human
      Sample Type: Cell Culture Supernates
    181. Toll-like receptor 10 is involved in induction of innate immune responses to influenza virus infection.
      Authors: Lee S, Kok K, Jaume M, Cheung T, Yip T, Lai J, Guan Y, Webster R, Jin D, Peiris J
      Proc Natl Acad Sci U S A, 2014;111(10):3793-8.
      Species: Human
      Sample Type: cell supernates
    182. Species-specific PAMP recognition by TLR2 and evidence for species-restricted interaction with Dectin-1.
      Authors: Willcocks S, Offord V, Seyfert H, Coffey T, Werling D
      J Leukoc Biol, 2013;94(3):449-58.
      Species: Bovine
      Sample Type: cell supernates
    183. Bacteria induce prolonged PMN survival via a phosphatidylcholine-specific phospholipase C- and protein kinase C-dependent mechanism.
      Authors: Erttmann S, Gekara N, Fallman M
      PLoS ONE, 2014;9(1):e87859.
      Species: Human
      Sample Type: Cell Culture Supernates
    184. Pathogenic E. coli exploits SslE mucinase activity to translocate through the mucosal barrier and get access to host cells.
      Authors: Valeri M, Rossi Paccani S, Kasendra M, Nesta B, Serino L, Pizza M, Soriani M
      PLoS ONE, 2015;10(3):e0117486.
      Species: Human
      Sample Type: cell supernates
    185. The C-terminal disulfide bonds of Helicobacter pylori GroES are critical for IL-8 secretion via the TLR4-dependent pathway in gastric epithelial cells.
      Authors: Su Y, Yang J, Lee H, Sheu F, Hsu C, Lin S, Chow L
      J Immunol, 2015;194(8):3997-4007.
      Species: Human
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    186. The ATP Receptors P2X7 and P2X4 Modulate High Glucose and Palmitate-Induced Inflammatory Responses in Endothelial Cells.
      Authors: Sathanoori R, Sward K, Olde B, Erlinge D
      PLoS ONE, 2015;10(5):e0125111.
      Species: Human
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    187. High EMT Signature Score of Invasive Non-Small Cell Lung Cancer (NSCLC) Cells Correlates with NFkappaB Driven Colony-Stimulating Factor 2 (CSF2/GM-CSF) Secretion by Neighboring Stromal Fibroblasts.
      Authors: Rudisch A, Dewhurst M, Horga L, Kramer N, Harrer N, Dong M, van der Kuip H, Wernitznig A, Bernthaler A, Dolznig H, Sommergruber W
      PLoS ONE, 0;10(4):e0124283.
      Species: Human
      Sample Type: Cell Culture Supernates
    188. SseK3 Is a Salmonella Effector That Binds TRIM32 and Modulates the Host's NF-kappaB Signalling Activity.
      Authors: Yang Z, Soderholm A, Lung T, Giogha C, Hill M, Brown N, Hartland E, Teasdale R
      PLoS ONE, 2015;10(9):e0138529.
      Species: Human
      Sample Type: Cell Culture Supernates
    189. Group B streptococcal infection and activation of human astrocytes.
      Authors: Stoner T, Weston T, Trejo J, Doran K
      PLoS ONE, 2015;10(6):e0128431.
      Species: Human
      Sample Type: Cell Lysates
    190. Dietary Omega-3 Fatty Acid Supplementation Reduces Inflammation in Obese Pregnant Women: A Randomized Double-Blind Controlled Clinical Trial.
      Authors: Haghiac M, Yang X, Presley L, Smith S, Dettelback S, Minium J, Belury M, Catalano P, Hauguel-De Mouzon S
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      Species: Human
      Sample Type: Plasma
    191. The Alpha-Melanocyte-Stimulating Hormone Suppresses TLR2-Mediated Functional Responses through IRAK-M in Normal Human Keratinocytes.
      Authors: Ryu S, Johnson A, Park Y, Kim B, Norris D, Armstrong C, Song P
      PLoS ONE, 2015;10(8):e0136887.
      Species: Human
      Sample Type: Cell Culture Supernates
    192. Icariin ameliorates cigarette smoke induced inflammatory responses via suppression of NF-kappaB and modulation of GR in vivo and in vitro.
      Authors: Li L, Sun J, Xu C, Zhang H, Wu J, Liu B, Dong J
      PLoS ONE, 2014;9(8):e102345.
      Species: Human
      Sample Type: Cell Culture Supernates
    193. S100A9 expressed in ER(-)PgR(-) breast cancers induces inflammatory cytokines and is associated with an impaired overall survival.
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      Br J Cancer, 2015;113(8):1234-43.
      Species: Human
      Sample Type: Cell Culture Supernates
    194. CRISPR-Cas9-mediated gene knockout in primary human airway epithelial cells reveals a proinflammatory role for MUC18.
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      Gene Ther, 2015;22(10):822-9.
      Species: Human
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