Human CXCR6 APC-conjugated Antibody

Clone 56811 was used by HLDA to establish CD designation.
(3 citations)
(2 Reviews)
  
  • Species Reactivity
    Human
  • Specificity
    Stains human CXCR6 transfectants but not irrelevant transfectants.
  • Source
    Monoclonal Mouse IgG2B Clone # 56811
  • Purification
    Protein A or G purified from ascites
  • Immunogen
    NS0 mouse myeloma cell line transfected with human CXCR6
    Met1-Leu342
    Accession # O00574
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
    Allophycocyanin
Applications
  •  
    Recommended
    Concentration
    Sample
  • Flow Cytometry
    10 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of CXCR6 in HEK293 Human Cell Line Transfected with Human CXCR6 by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with human CXCR6 was stained with Mouse Anti-Human CXCR6 APC‑conjugated Monoclonal Antibody (Catalog # FAB699A, filled histogram) or isotype control antibody (Catalog # IC0041A, open histogram). View our protocol for Staining Membrane-associated Proteins.
Detection of CXCR6 in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) either (A) untreated or (B) treated with 20 ng/mL Recombinant Human IL‑2 (Catalog # 202-IL) for 9 days were stained with Mouse Anti-Human CXCR6 APC‑conjugated Monoclonal Antibody (Catalog # FAB699A) and Mouse Anti-Human CD3 epsilon PE‑conjugated Monoclonal Antibody (Catalog # FAB100P). Quadrant markers were set based on control antibody staining (Catalog # IC0041A). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: CXCR6

CXCR6, also known as Bonzo and STRL33, is a G protein coupled chemokine receptor expressed on activated T cells and tumor-infiltrating lymphocytes. It binds soluble CXCL16 and membrane-anchored CXCL16 expressed on dendritic cells and macrophages.

  • Entrez Gene IDs:
    10663 (Human); 80901 (Mouse); 100124593 (Rat)
  • Alternate Names:
    Bonzo; BONZOG-protein coupled receptor bonzo; CD186 antigen; CD186; CDw186; chemokine (C-X-C motif) receptor 6; C-X-C chemokine receptor type 6; CXCR6; CXC-R6; CXCR-6; G protein-coupled receptor; STRL33; STRL33G-protein coupled receptor STRL33; TYMSTRCD186
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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Species
Applications
Sample Type
  1. IL-27 in human secondary lymphoid organs attracts myeloid dendritic cells and impairs HLA class I-restricted antigen presentation.
    Authors: Morandi, Fabio, Di Carlo, Emma, Ferrone, Soldano, Petretto, Andrea, Pistoia, Vito, Airoldi, Irma
    J Immunol, 2014;192(6):2634-42.
  2. Chemokine responsiveness of CD4+ CD25+ regulatory and CD4+ CD25- T cells from atopic and nonatopic donors.
    Authors: Ahern D, Lloyd CM, Robinson DS
    Allergy, 2009;64(8):1121-9.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  3. Expression of the CXCR6 on polymorphonuclear neutrophils in pancreatic carcinoma and in acute, localized bacterial infections.
    Authors: Gaida MM, Gunther F, Wagner C, Friess H, Giese NA, Schmidt J, Hansch GM, Wente MN
    Clin. Exp. Immunol., 2008;154(2):216-23.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
Isotype Controls
Description Application Cat# Citations Images  

Mouse IgG2B APC-conjugated Antibody

Ctrl IC0041A 4  
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Staining Reagents
Description Application Cat# Citations Images  

Flow Cytometry Staining Buffer (1X)

Flow FC001 6
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Flow Cytometry Mouse Lyse Buffer (10X)

Flow FC003 4
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Flow Cytometry Human Lyse Buffer (10X)

Flow FC002 1
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Average Rating: 4 (Based on 2 reviews)

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Read what people are saying who have used Human CXCR6 APC-conjugated Antibody.

We have 2 reviews tested in 1 species: Human.
We have 2 review tested in 1 application: Flow Cytometry.

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Images Ratings Applications Species Reviewed By Date Details
Flow Cytometry Human CXCR6 APC‑conjugated Antibody FAB699A
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Very Good
 Flow Human Anonymous 10/26/2015
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Image Details
Flow Cytometry Human CXCR6 APC‑conjugated Antibody FAB699A
Flow Cytometry: Human CXCR6 APC‑conjugated Antibody [FAB699A]

Summary

ApplicationFlow Cytometry
Sample TestedPeripheral blood cells
SpeciesHuman

Other Experimental Details

Other Experimental DetailsGated on lymphocyte FSC-A and SSC-A populations. Singlets were further gated on by FSC-A/FSC-H doublet exclusion.
Buffer: 2% Human Serum, 0.5 mM EDTA in PBS
Dilution: 1/100
Flow Cytometry Human CXCR6 APC‑conjugated Antibody FAB699A
Enlarge
Very Good
 Flow Human Anonymous 10/26/2015
Show
Image Details
Flow Cytometry Human CXCR6 APC‑conjugated Antibody FAB699A
Flow Cytometry: Human CXCR6 APC‑conjugated Antibody [FAB699A]

Summary

ApplicationFlow Cytometry
Sample TestedPeripheral blood mononuclear cells (PBMCs)
SpeciesHuman

Other Experimental Details

Other Experimental DetailsCells were stained for 10 minutes in room temperature followed by three washes.
Specificity: Specific
Sensitivity: Reasonably sensitive
Buffer: PBS 2.5% FBS
Dilution: 1/10

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