Human CXCR6 APC-conjugated Antibody

Clone 56811 was used by HLDA to establish CD designation
Catalog # Availability Size / Price Qty
FAB699A-025
FAB699A-100
Human CXCR6 APC-conjugated Antibody in Data
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Product Details
Citations (5)
FAQs
Supplemental Products
Reviews (2)

Human CXCR6 APC-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Stains human CXCR6 transfectants but not irrelevant transfectants.
Source
Monoclonal Mouse IgG2B Clone # 56811
Purification
Protein A or G purified from ascites
Immunogen
NS0 mouse myeloma cell line transfected with human CXCR6
Met1-Leu342
Accession # O00574
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Allophycocyanin (Excitation= 620-650 nm, Emission= 660-670 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Flow Cytometry Detection of CXCR6 in HEK293 Human Cell Line Transfected with Human CXCR6 by Flow Cytometry. View Larger

Detection of CXCR6 in HEK293 Human Cell Line Transfected with Human CXCR6 by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with human CXCR6 was stained with Mouse Anti-Human CXCR6 APC-conjugated Monoclonal Antibody (Catalog # FAB699A, filled histogram) or isotype control antibody (Catalog # IC0041A, open histogram). View our protocol for Staining Membrane-associated Proteins.

Flow Cytometry Detection of CXCR6 in Human PBMCs by Flow Cytometry. View Larger

Detection of CXCR6 in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) either (A) untreated or (B) treated with 20 ng/mL Recombinant Human IL-2 (Catalog # 202-IL) for 9 days were stained with Mouse Anti-Human CXCR6 APC-conjugated Monoclonal Antibody (Catalog # FAB699A) and Mouse Anti-Human CD3e PE-conjugated Monoclonal Antibody (Catalog # FAB100P). Quadrant markers were set based on control antibody staining (Catalog # IC0041A). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: CXCR6

CXCR6, also known as Bonzo and STRL33, is a G protein coupled chemokine receptor expressed on activated T cells and tumor-infiltrating lymphocytes. It binds soluble CXCL16 and membrane-anchored CXCL16 expressed on dendritic cells and macrophages.

Entrez Gene IDs
10663 (Human); 80901 (Mouse); 100124593 (Rat)
Alternate Names
Bonzo; BONZOG-protein coupled receptor bonzo; CD186 antigen; CD186; CDw186; chemokine (C-X-C motif) receptor 6; C-X-C chemokine receptor type 6; CXCR6; CXC-R6; CXCR-6; G protein-coupled receptor; STRL33; STRL33G-protein coupled receptor STRL33; TYMSTRCD186

Product Datasheets

Citations for Human CXCR6 APC-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

5 Citations: Showing 1 - 5
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  1. Resident memory T cells are a cellular reservoir for HIV in the cervical mucosa
    Authors: J Cantero-Pé, J Grau-Expós, C Serra-Pein, DA Rosero, L Luque-Ball, A Astorga-Ga, J Castellví, T Sanhueza, G Tapia, B Lloveras, MA Fernández, JG Prado, JM Solé-Seden, A Tarrats, C Lecumberri, L Mañalich-B, C Centeno-Me, V Falcó, MJ Buzon, M Genescà
    Nat Commun, 2019;10(1):4739.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  2. Role of CXCL13 and CCL20 in the recruitment of B cells to inflammatory foci in chronic arthritis
    Authors: E Armas-Gonz, MJ Domínguez-, A Díaz-Martí, M Arce-Franc, J Castro-Her, G Danelon, V Hernández-, S Bustabad-R, A Cantabrana, M Uguccioni, F Díaz-Gonzá
    Arthritis Res. Ther., 2018;20(1):114.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  3. IL-27 in human secondary lymphoid organs attracts myeloid dendritic cells and impairs HLA class I-restricted antigen presentation.
    Authors: Morandi, Fabio, Di Carlo, Emma, Ferrone, Soldano, Petretto, Andrea, Pistoia, Vito, Airoldi, Irma
    J Immunol, 2014;192(6):2634-42.
  4. Chemokine responsiveness of CD4+ CD25+ regulatory and CD4+ CD25- T cells from atopic and nonatopic donors.
    Authors: Ahern D, Lloyd CM, Robinson DS
    Allergy, 2009;64(8):1121-9.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  5. Expression of the CXCR6 on polymorphonuclear neutrophils in pancreatic carcinoma and in acute, localized bacterial infections.
    Authors: Gaida MM, Gunther F, Wagner C, Friess H, Giese NA, Schmidt J, Hansch GM, Wente MN
    Clin. Exp. Immunol., 2008;154(2):216-23.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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Reviews for Human CXCR6 APC-conjugated Antibody

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Human CXCR6 APC-conjugated Antibody
By Anonymous on 10/26/2015
Application: Flow Sample Tested: Peripheral blood cells Species: Human

Gated on lymphocyte FSC-A and SSC-A populations. Singlets were further gated on by FSC-A/FSC-H doublet exclusion.
Buffer: 2% Human Serum, 0.5 mM EDTA in PBS
Dilution: 1/100


Human CXCR6 APC-conjugated Antibody
By Anonymous on 10/26/2015
Application: Flow Sample Tested: Peripheral blood mononuclear cells (PBMCs) Species: Human

Cells were stained for 10 minutes in room temperature followed by three washes.
Specificity: Specific
Sensitivity: Reasonably sensitive
Buffer: PBS 2.5% FBS
Dilution: 1/10