Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Flow Cytometry

Cited:

Immunohistochemistry-Paraffin, Flow Cytometry

Label

Allophycocyanin (Excitation = 620-650 nm, Emission = 660-670 nm)

Antibody Source

Monoclonal Mouse IgG2B Clone # 56811
View all CXCR6 Primary Antibodies »

Product Specifications

Immunogen

NS0 mouse myeloma cell line transfected with human CXCR6
Met1-Leu342
Accession # O00574

Specificity

Stains human CXCR6 transfectants but not irrelevant transfectants.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Human CXCR6 APC‑conjugated Antibody

Detection of CXCR6 antibody in HEK293 Human Cell Line Transfected with Human CXCR6 antibody by Flow Cytometry.

Detection of CXCR6 in HEK293 Human Cell Line Transfected with Human CXCR6 by Flow Cytometry.

HEK293 human embryonic kidney cell line transfected with human CXCR6 was stained with Mouse Anti-Human CXCR6 APC-conjugated Monoclonal Antibody (Catalog # FAB699A, filled histogram) or isotype control antibody (Catalog # IC0041A, open histogram). View our protocol for Staining Membrane-associated Proteins.
Detection of CXCR6 antibody in Human PBMCs antibody by Flow Cytometry.

Detection of CXCR6 in Human PBMCs by Flow Cytometry.

Human peripheral blood mononuclear cells (PBMCs) either (A) untreated or (B) treated with 20 ng/mL Recombinant Human IL-2 (Catalog # 202-IL) for 9 days were stained with Mouse Anti-Human CXCR6 APC-conjugated Monoclonal Antibody (Catalog # FAB699A) and Mouse Anti-Human CD3e PE-conjugated Monoclonal Antibody (Catalog # FAB100P). Quadrant markers were set based on control antibody staining (Catalog # IC0041A). View our protocol for Staining Membrane-associated Proteins.
Detection of CXCR6 by Flow Cytometry

Detection of CXCR6 by Flow Cytometry

CXCR6 promotes BC cells migration and invasionA) CXCR6 was overexpressed in MCF-7 and MDA-231 BC cells by lentiviral system. CXCR6 -positive cells were then sorted by flow cytometry. Histogram displays the ratio of CXCR6 positive expression of MCF-7CXCR6, MDA-231CXCR6 and their respective control cells. B) Scratch wound healing assay shows that overexpression of CXCR6 enhanced cell migration in MDA-231 cells, but not very significant in MCF-7 cells. C) Matrigel invasion assay showed that overexpression of CXCR6 significantly enhanced cell invasion in both MCF-7 and MDA-231 cells (***P < 0.001). Experiments were performed 3 times; representative images of invaded cells are shown. Mean ± SD. D) Cytometry analysis showed CXCR6 had no effect on cell cycle. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/25909173), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Human CXCR6 APC‑conjugated Antibody

Application
Recommended Usage

Flow Cytometry

10 µL/106 cells
Sample: HEK293 human embryonic kidney cell line transfected with human CXCR6 and human peripheral blood mononuclear cells (PBMCs) treated with Recombinant Human IL‑2 (Catalog # 202-IL)

Reviewed Applications

Read 4 reviews rated 4.5 using FAB699A in the following applications:

Spectra Viewer

Plan Your Experiments

Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

Spectra Viewer
Spectra Viewer

Flow Cytometry Panel Builder

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Save time and reduce costly mistakes by quickly finding compatible reagents using the Panel Builder Tool.

Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from ascites

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: CXCR6

CXCR6, also known as Bonzo and STRL33, is a G protein coupled chemokine receptor expressed on activated T cells and tumor-infiltrating lymphocytes. It binds soluble CXCL16 and membrane-anchored CXCL16 expressed on dendritic cells and macrophages.

Alternate Names

Bonzo, CD186, CXCR6, STRL33

Entrez Gene IDs

10663 (Human); 80901 (Mouse); 100124593 (Rat)

Gene Symbol

CXCR6

UniProt

O00574

Additional CXCR6 Products

Product Documents for Human CXCR6 APC‑conjugated Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human CXCR6 APC‑conjugated Antibody

For research use only

Citations for Human CXCR6 APC‑conjugated Antibody

Customer Reviews for Human CXCR6 APC‑conjugated Antibody (4)

4.5 out of 5
4 Customer Ratings
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Customer Images

Showing  1 - 4 of 4 reviews Showing All
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  • Human CXCR6 APC‑conjugated Antibody
    Name: Anonymous
    Application: Flow Cytometry
    Sample Tested: Peripheral blood mononuclear cells (PBMCs)
    Species: Human
    Verified Customer | Posted 10/26/2015
    Cells were stained for 10 minutes in room temperature followed by three washes. <br />Specificity: Specific<br />Sensitivity: Reasonably sensitive<br />Buffer: PBS 2.5% FBS<br />Dilution: 1/10
    Human CXCR6 APC‑conjugated Antibody FAB699A
  • Human CXCR6 APC‑conjugated Antibody
    Name: Anonymous
    Application: Flow Cytometry
    Sample Tested: Peripheral blood cells
    Species: Human
    Verified Customer | Posted 10/26/2015
    Gated on lymphocyte FSC-A and SSC-A populations. Singlets were further gated on by FSC-A/FSC-H doublet exclusion. <br />Buffer: 2% Human Serum, 0.5 mM EDTA in PBS<br />Dilution: 1/100
    Human CXCR6 APC‑conjugated Antibody FAB699A
  • Name: Anonymous
    Application: Flow Cytometry
    Sample Tested: See PMID: 21203549
    Species: Human
    Verified Customer | Posted 01/27/2015
  • Name: Anonymous
    Application: Flow Cytometry
    Sample Tested: See PMID: 21203549
    Species: Human
    Verified Customer | Posted 01/27/2015

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