Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human

Cited:

Human, Primate - Macaca mulatta (Rhesus Macaque)

Applications

Validated:

Flow Cytometry, CyTOF-reported

Cited:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, Flow Cytometry, Immunocytochemistry, CyTOF-reported

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 56811
View all CXCR6 Primary Antibodies »

Product Specifications

Immunogen

NS0 mouse myeloma cell line transfected with human CXCR6
Met1-Leu342
Accession # O00574

Specificity

Stains human CXCR6 transfectants but not irrelevant transfectants.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Human CXCR6 Antibody

Detection of CXCR6 antibody in Human PBMCs antibody by Flow Cytometry.

Detection of CXCR6 in Human PBMCs by Flow Cytometry.

Human peripheral blood mononuclear cells (PBMCs) either (A) treated with 20 ng/mL Recombinant Human IL-2 (Catalog # 202-IL) for 6 days or (B) untreated were stained with Mouse Anti-Human CXCR6 Monoclonal Antibody (Catalog # MAB699) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B) and Mouse Anti-Human CD3e PE-conjugated Monoclonal Antibody (Catalog # FAB100P). Quadrant markers were set based on control antibody staining (Catalog # MAB0041). View our protocol for Staining Membrane-associated Proteins.
Detection of CXCR6 antibody in HEK293 Human cell line transfected with Human CXCR6 and eGFP antibody by Flow Cytometry.

Detection of CXCR6 in HEK293 Human cell line transfected with Human CXCR6 and eGFP by Flow Cytometry.

HEK293 Human cell line transfected with either (A) Human CXCR6 or (B) irrelevant transfectant and eGFP, was stained with Mouse Anti-Human CXCR6 Monoclonal Antibody (Catalog # MAB699) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). Quadrant markers were set based on control antibody staining (Catalog # MAB0041). View our protocol for Staining Membrane-associated Proteins.
Detection of Human CXCR6 by Immunocytochemistry/Immunofluorescence

Detection of Human CXCR6 by Immunocytochemistry/Immunofluorescence

CXCR6 expression in tissues samples from LuCa patients(A) Representative images for CXCR6 tissue expression in lung tissues from non-neoplastic (n = 8), adenocarcinoma (n = 54) and squamous cell carcinoma (n = 24) were stained with isotype control or anti-CXCR6 antibodies. Brown (DAB) color shows CXCR6 staining. Images were captured using tissuefaxs cell analysis system from Tissuegnostics. (B) Immuno-intensities of CXCR6 were quantified using image analysis Aperio ImageScope v.6.25 software using ImageScope algorithm. ***p ≤ 0.0001 when compared between groups. Image collected and cropped by CiteAb from the following publication (https://www.oncotarget.com/lookup/doi/10.18632/oncotarget.3194), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Human CXCR6 Antibody

Application
Recommended Usage

CyTOF-reported

Strauss-Albee, D.M. et al. (2015) Sci. Transl. Med. 7: 297ra115. Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

0.25 µg/106 cells
Sample: Human peripheral blood mononuclear cells (PBMCs) treated with Recombinant Human IL‑2 (Catalog # 202-IL) and HEK293 human cell line transfected with human CXCR6

Flow Cytometry Panel Builder

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Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from ascites

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: CXCR6

CXCR6, also known as Bonzo and STRL33, is a G protein coupled chemokine receptor expressed on activated T cells and tumor-infiltrating lymphocytes. It binds soluble CXCL16 and membrane-anchored CXCL16 expressed on dendritic cells and macrophages.

Alternate Names

Bonzo, CD186, CXCR6, STRL33

Entrez Gene IDs

10663 (Human); 80901 (Mouse); 100124593 (Rat)

Gene Symbol

CXCR6

UniProt

O00574

Additional CXCR6 Products

Product Documents for Human CXCR6 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human CXCR6 Antibody

For research use only

Citations for Human CXCR6 Antibody

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