hh Human CXCR6 Antibody MAB699-100: R&D Systems

Human CXCR6 Antibody

Clone 56811 was used by HLDA to establish CD designation.
(15 citations)   
  • Species Reactivity
    Human
  • Specificity
    Stains human CXCR6 transfectants but not irrelevant transfectants.
  • Source
    Monoclonal Mouse IgG2B Clone # 56811
  • Purification
    Protein A or G purified from ascites
  • Immunogen
    NS0 mouse myeloma cell line transfected with human CXCR6
    Met1-Leu342
    Accession # O00574
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Flow Cytometry
    2.5 µg/106 cells
    See below
  • CyTOF-reported
    Strauss-Albee, D.M. et al. (2015) Sci. Transl. Med. 7: 297ra115. Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of CXCR6 in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) either (A) untreated or (B) treated with 20 ng/mL Recombinant Human IL‑2 (Catalog # 202-IL) for 9 days were stained with Mouse Anti-Human CXCR6 Monoclonal Antibody (Catalog # MAB699) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B) and Mouse Anti-Human CD3 epsilon PE‑conjugated Monoclonal Antibody (Catalog # FAB100P). Quadrant markers were set based on control antibody staining (Catalog # MAB0041).
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CXCR6

CXCR6, also known as Bonzo and STRL33, is a G protein coupled chemokine receptor expressed on activated T cells and tumor-infiltrating lymphocytes. It binds soluble CXCL16 and membrane-anchored CXCL16 expressed on dendritic cells and macrophages.

  • Entrez Gene IDs:
    10663 (Human); 80901 (Mouse); 100124593 (Rat)
  • Alternate Names:
    Bonzo; BONZOG-protein coupled receptor bonzo; CD186 antigen; CD186; CDw186; chemokine (C-X-C motif) receptor 6; C-X-C chemokine receptor type 6; CXCR6; CXC-R6; CXCR-6; G protein-coupled receptor; STRL33; STRL33G-protein coupled receptor STRL33; TYMSTRCD186
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

15 Citations: Showing 1 - 10
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Species
Applications
Sample Type
  1. The Chemokine Receptor CXCR6 Evokes Reverse Signaling via the Transmembrane Chemokine CXCL16
    Authors: V Adamski, R Mentlein, R Lucius, M Synowitz, J Held-Feind, K Hattermann
    Int J Mol Sci, 2017;18(7):.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
  2. Chemoprophylaxis with sporozoite immunization in P. knowlesi rhesus monkeys confers protection and elicits sporozoite-specific memory T cells in the liver
    Authors: S Pichyangku, MD Spring, K Yongvanitc, U Kum-Arb, A Limsalakpe, R Im-Erbsin, R Ubalee, P Vanachayan, EJ Remarque, E Angov, PL Smith, DL Saunders
    PLoS ONE, 2017;12(2):e0171826.
    Species: Primate - Macaca mulatta (Rhesus Macaque)
    Sample Type: Whole Cells
    Application: Flow
  3. CXCL16 and CXCR6 are coexpressed in human lung cancer in vivo and mediate the invasion of lung cancer cell lines in vitro.
    Authors: Hu, Weidong, Liu, Yue, Zhou, Wenhui, Si, Lianlian, Ren, Liang
    PLoS ONE, 2014;9(6):e99056.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
  4. Chemokine-enhanced chemotaxis of lymphangioleiomyomatosis cells with mutations in the tumor suppressor TSC2 gene.
    Authors: Pacheco-Rodriguez G, Kumaki F, Steagall WK, Zhang Y, Ikeda Y, Lin JP, Billings EM, Moss J
    J. Immunol., 2009;182(3):1270-7.
    Species: Human
    Sample Type: Whole Tissue
    Application: IHC Paraffin-embedded
  5. Overexpression of CXCL16 and its receptor CXCR6/Bonzo promotes growth of human schwannomas.
    Authors: Held-Feindt J, Rehmke B, Mentlein R, Hattermann K, Knerlich F, Hugo HH, Ludwig A, Mehdorn HM
    Glia, 2008;56(7):764-74.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  6. Expression and potential function of the CXC chemokine CXCL16 in pancreatic ductal adenocarcinoma.
    Authors: Wente MN, Gaida MM, Mayer C, Michalski CW, Haag N, Giese T, Felix K, Bergmann F, Giese NA, Friess H
    Int. J. Oncol., 2008;33(2):297-308.
    Species: Human
    Sample Type: Whole Tissue
    Application: IHC Paraffin-embedded
  7. Expression of the CXCR6 on polymorphonuclear neutrophils in pancreatic carcinoma and in acute, localized bacterial infections.
    Authors: Gaida MM, Gunther F, Wagner C, Friess H, Giese NA, Schmidt J, Hansch GM, Wente MN
    Clin. Exp. Immunol., 2008;154(2):216-23.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  8. Expression and role of CCR6/CCL20 chemokine axis in pulmonary sarcoidosis.
    Authors: Facco M, Baesso I, Miorin M, Bortoli M, Cabrelle A, Boscaro E, Gurrieri C, Trentin L, Zambello R, Calabrese F, Cassatella MA, Semenzato G, Agostini C
    J. Leukoc. Biol., 2007;82(4):946-55.
    Species: Human
    Sample Type: Whole Tissue
    Application: IHC Frozen
  9. CD molecules 2006--human cell differentiation molecules.
    Authors: Zola H, Swart B, Banham A, Barry S, Beare A, Bensussan A, Boumsell L, D Buckley C, Buhring HJ, Clark G, Engel P, Fox D, Jin BQ, Macardle PJ, Malavasi F, Mason D, Stockinger H, Yang X
    J. Immunol. Methods, 2007;319(1):1-5.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  10. Vitamin A metabolites induce gut-homing FoxP3+ regulatory T cells.
    Authors: Kang SG, Lim HW, Andrisani OM, Broxmeyer HE, Kim CH
    J. Immunol., 2007;179(6):3724-33.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  11. The majority of human peripheral blood CD4+CD25highFoxp3+ regulatory T cells bear functional skin-homing receptors.
    Authors: Hirahara K, Liu L, Clark RA, Yamanaka K, Fuhlbrigge RC, Kupper TS
    J. Immunol., 2006;177(7):4488-94.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  12. TLR4-NOX4-AP-1 signaling mediates lipopolysaccharide-induced CXCR6 expression in human aortic smooth muscle cells.
    Authors: Patel DN, Bailey SR, Gresham JK, Schuchman DB, Shelhamer JH, Goldstein BJ, Foxwell BM, Stemerman MB, Maranchie JK, Valente AJ, Mummidi S, Chandrasekar B
    Biochem. Biophys. Res. Commun., 2006;347(4):1113-20.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  13. CXCL16-mediated cell recruitment to rheumatoid arthritis synovial tissue and murine lymph nodes is dependent upon the MAPK pathway.
    Authors: Ruth JH, Haas CS, Park CC, Amin MA, Martinez RJ, Haines GK, Shahrara S, Campbell PL, Koch AE
    Arthritis Rheum., 2006;54(3):765-78.
    Species: Human
    Sample Type: Whole Tissue
    Application: IHC
  14. Regulatory T cells can migrate to follicles upon T cell activation and suppress GC-Th cells and GC-Th cell-driven B cell responses.
    Authors: Lim HW, Hillsamer P, Kim CH
    J. Clin. Invest., 2004;114(11):1640-9.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
  15. Possible link between unique chemokine and homing receptor expression at diagnosis and relapse location in a patient with childhood T-ALL.
    Authors: Annels NE, Willemze AJ, van der Velden VH, Faaij CM, van Wering E, Sie-Go DM, Egeler RM, van Tol MJ, Révész T
    Blood, 2003;103(7):2806-8.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
Expand to show all 15 Citations
Isotype Controls
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Mouse IgG2B Isotype Control

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Mouse IgG2B Isotype Control

Ctrl MAB0041 7  
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Mouse IgG2B Isotype Control

Ctrl MAB0042 6
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Secondary Antibodies
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Mouse IgG HRP-conjugated Antibody

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Mouse F(ab)2 IgG (H+L) PE-conjugated Antibody

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Donkey Anti-Mouse IgG NorthernLights™ NL557-conjugate Antibody

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Donkey Anti-Mouse IgG Biotinylated Antibody

WB BAF018 1
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Mouse IgG HRP-conjugated Antibody

WB HAF018 9  
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Donkey Anti-Mouse IgG NorthernLights™ NL637-conjugated Antibody

Flow, IHC, ICC NL008 8
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Mouse F(ab)2 IgG (H+L) APC-conjugated Antibody

Flow F0101B 2  
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Donkey Anti-Mouse IgG NorthernLights™ NL493-conjugated Antibody

Flow, IHC, ICC NL009 5
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Mouse F(ab)2 IgG (H+L) Fluorescein-conjugated Antibody

Flow F0103B 5  
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Mouse IgG Antibody

WB AF007 4
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Goat Anti-Mouse IgG Biotinylated Antibody

WB BAF007 4
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Mouse/Rabbit IgG VisUCyte HRP Polymer Antibody

IHC VC002  
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Rat Anti-Mouse IgG2B APC-conjugated Antibody

Flow F0133 1  
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Mouse IgG VisUCyte HRP Polymer Antibody

IHC VC001  
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Goat Anti-Mouse IgG Fc Affinity Purified Polyclonal Ab

G-202-C 2
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Mouse F(ab)2 IgG (H+L) PerCP-conjugated Antibody

Flow F0114  
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Rat Anti-Mouse IgG2B Fluorescein-conjugated Antibody

Flow F0134 1
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Rat Anti-Mouse IgG2B PE-conjugated Antibody

Flow F0132  
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Mouse IgG2B Antibody

Flow MAB0043  
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