Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Western Blot, Immunocytochemistry, Simple Western

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2A Clone # 817815
View all Cyclophilin A Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human Cyclophilin A
Met1-Glu165
Accession # P62937

Specificity

Detects human Cyclophilin A in direct ELISAs and Western blots. Detects mouse and rat Cyclophilin in Western blots. In direct ELISAs, approximately 50% cross-reactivity with recombinant human (rh) Cyclophilin E, less than 15% cross-reactivity with rhCyclophilin F, and less than 5% cross-reactivity with rhCyclophilin A-like 4 is observed.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2A

Scientific Data Images for Human Cyclophilin A Antibody

Detection of Human, Mouse, and Rat Cyclophilin A antibody by Western Blot.

Detection of Human, Mouse, and Rat Cyclophilin A by Western Blot.

Western blot shows lysates of MOLT-4 human acute lymphoblastic leukemia cell line, HeLa human cervical epithelial carcinoma cell line, C2C12 mouse myoblast cell line, Nb2-11 rat lymphoma cell line, and BaF3 mouse pro-B cell line. PVDF membrane was probed with 0.1 µg/mL of Rat Anti-Human Cyclophilin A Monoclonal Antibody (Catalog # MAB3589) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for Cyclophilin A at approximately 18 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Cyclophilin A antibody in PANC-1 Human Cell Line by Immunocytochemistry (ICC).

Cyclophilin A in PANC-1 Human Cell Line.

Cyclophilin A was detected in immersion fixed PANC-1 human pancreatic carcinoma cell line using Rat Anti-Human Cyclophilin A Monoclonal Antibody (Catalog # MAB3589) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL013) and counterstained with DAPI (blue). Specific staining was localized to nuclei and cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Detection of Human Cyclophilin A by Simple WesternTM.

Simple Western shows lysates of MOLT‑4 human acute lymphoblastic leukemia cell line, loaded at 0.5 mg/ml. A specific band was detected for Cyclophilin A at approximately 22 kDa (as indicated) using 10 µg/mL of Rat Anti-Human Cyclophilin A Monoclonal Antibody (Catalog # MAB3589). This experiment was conducted under reducing conditions and using the 2-40kDa separation system.

Applications for Human Cyclophilin A Antibody

Application
Recommended Usage

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed PANC1 human pancreatic carcinoma cell line

Simple Western

10 µg/mL
Sample: MOLT-4 human acute lymphoblastic leukemia cell line

Western Blot

0.1 µg/mL
Sample: MOLT‑4 human acute lymphoblastic leukemia cell line, HeLa human cervical epithelial carcinoma cell line, C2C12 mouse myoblast cell line, Nb2‑11 rat lymphoma cell line, and BaF3 mouse pro-B cell line

Reviewed Applications

Read 1 review rated 5 using MAB3589 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Cyclophilin A

Cyclophilin A, also called Peptidyl-prolyl Isomerase A, PPIA, CYPA, and CYPH, was originally characterized for its ability to catalyze the transition between cis- and trans- proline residues critical for proper folding of proteins (1). Cyclophilin is also incorporated into many viruses, including HIV-1, where it has been speculated to be involved in functions such as viral assembly and infectivity (2). The immunosuppressive activity of cyclosporins has been correlated with their ability to form complexes with cyclophilins that inhibit calcineurin phosphatase activity (3) and prevent incorporation of cyclophilin into viral particles (4). The cyclosporin/cyclophilin complex selectively binds and inactivates calcineurin (3, 5), making it a useful inhibitor for studying calcineurin activity.

References

  1. Hamilton, G.S. and J.P. Steiner  (1998) J. Med. Chem. 41:5119.
  2. Cantin, R. et al. (2005) J. Virology 79:6577.
  3. Liu, J. et al. (1992) Biochemistry 31:3896.
  4. Wiegers K. and H.G. Krausslich (2002) Virology 294:289.
  5. Liu, J. et al. (1991) Cell 66:807.

Alternate Names

CYPA, CYPH, PPIA, Rotamase

Entrez Gene IDs

5478 (Human); 268373 (Mouse); 25518 (Rat)

Gene Symbol

PPIA

UniProt

P62937

Additional Cyclophilin A Products

Product Documents for Human Cyclophilin A Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human Cyclophilin A Antibody

For research use only

Citations for Human Cyclophilin A Antibody

Customer Reviews for Human Cyclophilin A Antibody (1)

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  • Human Cyclophilin A Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: HeLa human cervical epithelial carcinoma cell line
    Species: Human
    Verified Customer | Posted 11/28/2021
    Human Cyclophilin A Antibody MAB3589

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