Human DAP12 Antibody Summary
Accession # O43914
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of DAP12 in Human CD56+Natural Killer Cells by Flow Cytometry. Human peripheral blood CD56+natural killer cells were stained with Human DAP12 Monoclonal Antibody (Catalog # MAB5240, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG F(ab')2Secondary Antibody (Catalog # F0101B). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.
Detection of Human DAP12 by Western Blot. Western blot shows lysates of NS0 mouse myeloma cell line, mock transfected, and primary human CD56+NK cells. PVDF membrane was probed with 5 µg/mL of Human DAP12 Monoclonal Antibody (Catalog # MAB5240) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for DAP12 at approximately 10 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
DAP12, also known as TYROBP and KARAP, is a transmembrane protein that functions as a signal transduction adaptor molecule. DAP12 is expressed as a disulfide-linked homodimer that associates with a variety of receptors on NK and myeloid cells. Complex formation is mediated by intramembrane ionic interaction between an aspartic acid residue in DAP12 and a lysine residue in the partnered receptor. Ligation of these receptors can trigger either cell activation or inhibition through the ITAM sequence in DAP12, resulting in activation of Src family tyrosine kinases. Human and mouse DAP12 share 73% amino acid sequence identity.
Citations for Human DAP12 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
Citations: Showing 1 - 2
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Expansion and activation of distinct central memory T lymphocyte subsets in complex regional pain syndrome
Authors: MA Russo, NT Fiore, C van Vreden, D Bailey, DM Santarelli, HM McGuire, B Fazekas de, PJ Austin
J Neuroinflammation, 2019;16(1):63.
Sample Types: Whole Cells
CD33 modulates TREM2: convergence of Alzheimer loci.
Authors: Chan G, White C, Winn P, Cimpean M, Replogle J, Glick L, Cuerdon N, Ryan K, Johnson K, Schneider J, Bennett D, Chibnik L, Sperling R, Bradshaw E, De Jager P
Nat Neurosci, 0;18(11):1556-8.
Sample Types: Whole Cells
Applications: Flow Cytometry
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