Human DAP12 Antibody

Catalog # Availability Size / Price Qty
MAB5240
MAB5240-SP
Human DAP12 Antibody in Data
2 Images
Product Details
Citations (2)
FAQs
Supplemental Products
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Human DAP12 Antibody Summary

Species Reactivity
Human
Specificity
Detects human DAP12 in Western blots.
Source
Monoclonal Mouse IgG1 Clone # 406288
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Human Dap12 synthetic peptide
QGQRSDVYSDLNTQRPYYK
Accession # O43914
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS and NaCl with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
5 µg/mL
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 
Intracellular Staining by Flow Cytometry
0.25 µg/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Intracellular Staining by Flow Cytometry Detection of DAP12 in Human CD56<SUP>+</SUP>Natural Killer Cells by Flow Cytometry. View Larger

Detection of DAP12 in Human CD56+Natural Killer Cells by Flow Cytometry. Human peripheral blood CD56+natural killer cells were stained with Human DAP12 Monoclonal Antibody (Catalog # MAB5240, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG F(ab')2Secondary Antibody (Catalog # F0101B). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.

Western Blot Detection of Human DAP12 by Western Blot. View Larger

Detection of Human DAP12 by Western Blot. Western blot shows lysates of NS0 mouse myeloma cell line, mock transfected, and primary human CD56+NK cells. PVDF membrane was probed with 5 µg/mL of Human DAP12 Monoclonal Antibody (Catalog # MAB5240) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for DAP12 at approximately 10 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: DAP12

DAP12, also known as TYROBP and KARAP, is a transmembrane protein that functions as a signal transduction adaptor molecule. DAP12 is expressed as a disulfide-linked homodimer that associates with a variety of receptors on NK and myeloid cells. Complex formation is mediated by intramembrane ionic interaction between an aspartic acid residue in DAP12 and a lysine residue in the partnered receptor. Ligation of these receptors can trigger either cell activation or inhibition through the ITAM sequence in DAP12, resulting in activation of Src family tyrosine kinases. Human and mouse DAP12 share 73% amino acid sequence identity.

Long Name
DNAX-activation Protein 12
Entrez Gene IDs
7305 (Human); 22177 (Mouse); 361537 (Rat)
Alternate Names
DAP12; DAP12PLOSL; DNAX-activation protein 12; KARAP; KARAPPLO-SL; KAR-associated protein; killer activating receptor associated protein; Killer-activating receptor-associated protein; TYRO protein tyrosine kinase binding protein; TYRO protein tyrosine kinase-binding protein; TYROBP

Product Datasheets

Citations for Human DAP12 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. Expansion and activation of distinct central memory T lymphocyte subsets in complex regional pain syndrome
    Authors: MA Russo, NT Fiore, C van Vreden, D Bailey, DM Santarelli, HM McGuire, B Fazekas de, PJ Austin
    J Neuroinflammation, 2019;16(1):63.
    Species: Human
    Sample Types: Whole Cells
    Applications: CyTOF
  2. CD33 modulates TREM2: convergence of Alzheimer loci.
    Authors: Chan G, White C, Winn P, Cimpean M, Replogle J, Glick L, Cuerdon N, Ryan K, Johnson K, Schneider J, Bennett D, Chibnik L, Sperling R, Bradshaw E, De Jager P
    Nat Neurosci, 0;18(11):1556-8.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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Isotype Controls

Reconstitution Buffers

Secondary Antibodies

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