Human DCLK1 Antibody

Catalog # Availability Size / Price Qty
AF7138
AF7138-SP
Detection of Human DCLK1 by Western Blot.
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Human DCLK1 Antibody Summary

Species Reactivity
Human
Specificity
Detects human DCLK1 in direct ELISAs and Western blots.
Source
Polyclonal Sheep IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human DCLK1 Isoform 1
Lys621-Met729
Accession # NP_004725
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below
Immunohistochemistry
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Western Blot Detection of Human DCLK1 by Western Blot. View Larger

Detection of Human DCLK1 by Western Blot. Western blot shows recombinant human (rh) DCLK1. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human DCLK1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7138) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for rhDCLK1 Isoform 1 (amino acids 621-729) at approximately 18 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Immunohistochemistry DCLK1 in Human Brain. View Larger

DCLK1 in Human Brain. DCLK1 was detected in formalin fixed paraffin-embedded sections of human brain (hippocampus) using Sheep Anti-Human DCLK1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7138) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to neurons. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Sterile PBS to a final concentration of 0.2 mg/mL.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: DCLK1

DCLK1 (Doublecortin-like and CAM Kinase-like 1; also DCAMKL1) is an 80-88 kDa member of the CaMK subfamily, Ser/Thr protein kinase family of molecules. It is found in both fetal and adult tissues, and individual cells expressing DCLK1 include pancreatic stem cells, intestinal Tuft cells, migrating neurons and select tumor types. DCLK1 is anchored to microtubules, and appears to induce tubulin polymerization. It also possesses a microtubule-independent Ser/Thr kinase phosphorylation activity. Human DCKL1 is 740 amino acids (aa) in length. It contains two doublecortin domains (aa 57-143 and 186-269) plus a protein kinase domain (aa 390-647). There are at least five utilized Ser/Thr phosphorylation sites. Multiple splice variants exist. One is termed DCL, and is a 42 kDa isoform that possesses an 18 aa substitution for aa 346-740. A second is called CPG-16 and represents a 50 kDa isoform that contains a six aa substitution for aa 1-313. A third isoform is called CARP, and is a combination of the C- and N-terminal substitutions described above. A fourth isoform resembles CPG-16 with a 43 aa substitution for aa 687-740, while a fifth isoform is simply DCLK1 with the same 43 aa substitution. DCLK1 undergoes proteolysis to generate a 35 kDa N-terminus and a 50 kDa C-terminus. Over aa 61-729, human DCLK1 shares 98% aa identity with mouse DCLK1.

Long Name
Doublecortin-like and CAM Kinase-like 1
Entrez Gene IDs
9201 (Human); 13175 (Mouse)
Alternate Names
CL1; CLICK1; DCAMKL1; DCAMKL1CLICK1; DCDC3A; DCDC3ACL1; DCLK; DCLK1; doublecortin and CaM kinase-like 1; Doublecortin domain-containing protein 3A; doublecortin-like kinase 1Doublecortin-like and CAM kinase-like 1; EC 2.7.11; KIAA0369EC 2.7.11.1; serine/threonine-protein kinase DCLK1

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Cell and Tissue Staining Kits

Immunohistochemistry Reagents

Isotype Controls

Reconstitution Buffers

Secondary Antibodies

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