Human DDR1 Antibody

Catalog # Availability Size / Price Qty
AF2396
AF2396-SP
Human DDR1 Antibody in Western Blot
3 Images
Product Details
Citations (3)
FAQs
Supplemental Products
Reviews

Human DDR1 Antibody Summary

Species Reactivity
Human
Specificity
Detects human DDR1 in direct ELISAs and Western blots. In sandwich immunoassays, approximately 5% cross-reactivity with recombinant human DDR2 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant human DDR1
Asp21-Thr416
Accession # Q08345
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below
Simple Western
10 µg/mL
See below
Immunohistochemistry
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Western Blot Detection of Human DDR1 by Western Blot. View Larger

Detection of Human DDR1 by Western Blot. Western blot shows lysates of K562 human chronic myelogenous leukemia cell line and MCF‑7 human breast cancer cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human DDR1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2396) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). Specific bands were detected for DDR1 at approximately 100 and 120 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunohistochemistry DDR1 in Human Breast Cancer Tissue. View Larger

DDR1 in Human Breast Cancer Tissue. DDR1 was detected in immersion fixed paraffin-embedded sections of human breast cancer tissue using Human DDR1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2396) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Simple Western Detection of Human DDR1 by Simple WesternTM. View Larger

Detection of Human DDR1 by Simple WesternTM. Simple Western lane view shows lysates of MCF‑7 human breast cancer cell line, loaded at 0.2 mg/mL. A specific band was detected for DDR1 at approximately 137 kDa (as indicated) using 10 µg/mL of Goat Anti-Human DDR1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2396) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the
12-230 kDa separation system.        

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: DDR1

DDR1, also known as CAK, CD167a, RTK6, and TrkE, is a 120-140 kDa type I transmembrane glycoprotein that belongs to the discoidin-like domain containing subfamily of receptor tyrosine kinases. Mature human DDR2 consists of a 398 amino acid (aa) extracellular domain (ECD) that includes the discoidin-like domain, a 27 aa transmembrane segment, and a 470 aa cytoplasmic region with a tyrosine kinase domain. Within the ECD, human DDR1 shares 53% aa sequence identity with human DDR2 and 93% with mouse and rat DDR1. DDR1 is expressed on epithelial tissues, activated monocytes and neutrophils, and in several cancers. Compared to isoform DDR1b, DDR1a lacks 37 aa’s that include a Shc-interacting NPxY motif in the cytoplasmic juxtamembrane region. Two additional kinase deficient splice forms are expressed in colon cancer. The discoidin-like domain mediates binding to collagens I-V. DDR1 selectively recognizes the triple helical structure of collagen. It is expressed on the cell surface as a dimer which can include different isoforms. DDR1 oligomerization enhances collagen binding and also modulates collagen fibrillogenesis. The transmembrane segment contains a leucine zipper and GxxxG motif, but neither is exclusively required for dimerization. Collagen binding induces prolonged autophosphorylation, including the NPxY motif. Collagen binding also results in the proteolytic cleavage of a tyrosine phosphorylated 60 kDa C-terminal fragment (CTF), and a 60 kDa ECD fragment. TIMP3 and TAPI-1 inhibit shedding of the ECD fragment but not the CTF. Over-expression of DDR1a promotes MMP-2 activation and results in an increased invasiveness of a glioblastoma cell line; DDR1b does not.

Long Name
Discoidin Domain Receptor 1
Entrez Gene IDs
780 (Human)
Alternate Names
CAK; CD167 antigen-like family member A; CD167a antigen; CD167a; DDR; DDR1; discoidin domain receptor family, member 1; discoidin domain receptor tyrosine kinase 1; Discoidin receptor tyrosine kinase; EC 2.7.10; EC 2.7.10.1; EDDR1; ENTRK4; Epithelial discoidin domain receptor 1; HGK2; MCK10; MCK-10; NTRK4; Protein-tyrosine kinase 3A; PTK3A protein tyrosine kinase 3A; PTK3A; receptor, type 4; RTK6; TRK E; TrkE

Product Datasheets

Citations for Human DDR1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
Filter your results:

Filter by:

  1. Cancer Cell Invasion in Three-dimensional Collagen Is Regulated Differentially by Galpha13 Protein and Discoidin Domain Receptor 1-Par3 Protein Signaling.
    Authors: Chow C, Ebine K, Knab L, Bentrem D, Kumar K, Munshi H
    J Biol Chem, 2016;291(4):1605-18.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  2. Shedding of discoidin domain receptor 1 by membrane-type matrix metalloproteinases.
    Authors: Fu, Hsueh-Li, Sohail, Anjum, Valiathan, Rajeshwa, Wasinski, Benjamin, Kumarasiri, Malika, Mahasenan, Kiran V, Bernardo, M Margar, Tokmina-Roszyk, Dorota, Fields, Gregg B, Mobashery, Shahriar, Fridman, Rafael
    J Biol Chem, 2013;288(17):12114-29.
  3. Type II collagen levels correlate with mineralization by articular cartilage vesicles.
    Authors: Jubeck B, Muth E, Gohr CM, Rosenthal AK
    Arthritis Rheum., 2009;60(9):2741-6.
    Species: Porcine
    Sample Types: Whole Tissue
    Applications: Western Blot

FAQs

No product specific FAQs exist for this product, however you may

View all Antibody FAQs

Cell and Tissue Staining Kits

Immunohistochemistry Reagents

Isotype Controls

Reconstitution Buffers

Secondary Antibodies

Reviews for Human DDR1 Antibody

There are currently no reviews for this product. Be the first to review Human DDR1 Antibody and earn rewards!

Have you used Human DDR1 Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review