Key Product Details

Species Reactivity

Human

Applications

Western Blot, Immunocytochemistry

Label

Unconjugated

Antibody Source

Polyclonal Sheep IgG
View all DISP1 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human DISP1
Glu212-Lys492, Ser746-Thr984
Accession # NP_116279

Specificity

Detects human DISP1 in direct ELISAs and Western blots.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Scientific Data Images for Human DISP1 Antibody

DISP1 antibody in BG01V Human Embryonic Stem Cells by Immunocytochemistry (ICC).

DISP1 in BG01V Human Embryonic Stem Cells.

DISP1 was detected in immersion fixed BG01V human embryonic stem cells using Sheep Anti-Human DISP1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3549) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Applications for Human DISP1 Antibody

Application
Recommended Usage

Immunocytochemistry

5-15 µg/mL
Sample: Immersion fixed BG01V human embryonic stem cells

Western Blot

0.1 µg/mL
Sample: Recombinant Human DISP1

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Background: DISP1

DISP1 is a multispan (12) transmembrane protein with cytoplasmic N- and C-terminal tails. It contains a stero/sensing domain (SSD) and has been shown to play a role in regulating the release of cholesterol-modified SHH. Within the regions used for immunization, human and mouse DISP1 share 93% amino acid sequence homology.

Long Name

Dispatched 1

Alternate Names

DISPA, Dispatched

Entrez Gene IDs

84976 (Human); 68897 (Mouse); 289338 (Rat)

Gene Symbol

DISP1

UniProt

NP_116279

Additional DISP1 Products

Product Documents for Human DISP1 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human DISP1 Antibody

For research use only

Citations for Human DISP1 Antibody

Customer Reviews for Human DISP1 Antibody

There are currently no reviews for this product. Be the first to review Human DISP1 Antibody and earn rewards!

Have you used Human DISP1 Antibody?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies