Human DISP1 Antibody

Catalog #: AF3549 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF3549
AF3549-SP

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DISP1 in BG01V Human Embryonic Stem Cells.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human DISP1 Antibody Summary

Species Reactivity

Human

Specificity

Detects human DISP1 in direct ELISAs and Western blots.

Source

Polyclonal Sheep IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human DISP1
Glu212-Lys492, Ser746-Thr984
Accession # NP_116279

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Human DISP1

Immunocytochemistry

5-15 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunocytochemistry

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DISP1 in BG01V Human Embryonic Stem Cells. DISP1 was detected in immersion fixed BG01V human embryonic stem cells using Sheep Anti-Human DISP1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3549) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Reconstitution Buffer Available

Reconstitution Buffer 1 (PBS)

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: DISP1

DISP1 is a multispan (12) transmembrane protein with cytoplasmic N- and C-terminal tails. It contains a stero/sensing domain (SSD) and has been shown to play a role in regulating the release of cholesterol-modified SHH. Within the regions used for immunization, human and mouse DISP1 share 93% amino acid sequence homology.

Long Name

Dispatched 1

Entrez Gene IDs

84976 (Human); 68897 (Mouse); 289338 (Rat)

Alternate Names

DISP1; DISPA; DISPAFLJ43740; dispatched A; dispatched homolog 1 (Drosophila); Dispatched; DKFZp434I0428; MGC104180; MGC13130; MGC16796; protein dispatched homolog 1