Human EGR1 Antibody

Catalog # Availability Size / Price Qty
MAB28181-100
MAB28181-SP
Human EGR1 Antibody in Immunohistochemistry
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Human EGR1 Antibody Summary

Species Reactivity
Human
Specificity
Detects human EGR1 in direct ELISAs.
Source
Monoclonal Rat IgG2A Clone # 406910
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human EGR1
Gln282-Ser433
Accession # P18146
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Immunohistochemistry
5-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Immunohistochemistry EGR1  in Human Hodgkin's Lymphoma. View Larger

EGR1 in Human Hodgkin's Lymphoma. EGR1 was detected in immersion fixed paraffin-embedded sections of human Hodgkin's Lymphoma using Rat Anti-Human EGR1 Monoclonal Antibody (Catalog # MAB28181) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to nuclei. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Immunohistochemistry EGR1  in Human Adenocarcinoma Tissue. View Larger

EGR1 in Human Adenocarcinoma Tissue. EGR1 was detected in immersion fixed paraffin-embedded sections of human adenocarcinoma tissue using Rat Anti-Human EGR1 Monoclonal Antibody (Catalog # MAB28181) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to nuclei. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: EGR1

Early Growth Response 1, (EGR1) belongs to the EGR family of zinc finger transcription factors and regulates the expression of several tumor suppressors. Its expression is induced by diverse signals that initiate growth and differentiation. EGR1 suppresses or activates cell growth depending on the tumor type. Within the region that includes its three zinc finger domains, human EGR1 shares 99% aa sequence identity with mouse and rat EGR1.

Long Name
Early Growth Response 1
Entrez Gene IDs
1958 (Human)
Alternate Names
AT225Transcription factor ETR103; early growth response 1; early growth response protein 1; EGR1; EGR-1; G0S30; KROX24; KROX-24; Nerve growth factor-induced protein A; NGFIA; NGFI-ATranscription factor Zif268; TIS8; ZIF-268; Zinc finger protein Krox-24; ZNF225; ZNF225Zinc finger protein 225

Product Datasheets

FAQs

No product specific FAQs exist for this product, however you may

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Cell and Tissue Staining Kits

Immunohistochemistry Reagents

Isotype Controls

Reconstitution Buffers

Secondary Antibodies

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