Human Endoglin/CD105 Quantikine ELISA Kit

R&D Systems | Catalog # DNDG00

R&D Systems
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Key Product Details

Assay Length

4.5 hours

Sample Type & Volume Required Per Well

Cell Culture Supernates (50 µL), Serum (50 µL), EDTA Plasma (50 µL), Heparin Plasma (50 µL)

Sensitivity

0.03 ng/mL

Assay Range

0.156-10 ng/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)
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Product Summary for Human Endoglin/CD105 Quantikine ELISA Kit

The Quantikine Human Endoglin Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human Endoglin in cell culture supernates, serum, and plasma. It contains NS0-expressed recombinant human Endoglin and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human Endoglin showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring Endoglin.

Product Specifications

Assay Type

Solid Phase Sandwich ELISA

Format

96-well strip plate

Measurement

Quantitative ELISA

Detection Method

Colorimetric - 450nm (TMB)

Conjugate

HRP

Species

Human

Specificity

Natural and recombinant human Endoglin

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Cell Culture Supernates, EDTA Plasma, Heparin Plasma, Serum

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 40 40 40
Mean (ng/mL) 0.617 1.67 3.14 0.659 1.80 3.47
Standard Deviation 0.017 0.053 0.094 0.044 0.117 0.220
CV% 2.8 3.2 3.0 6.7 6.5 6.3

Recovery for Human Endoglin/CD105 Quantikine ELISA Kit

The recovery of Endoglin spiked to levels throughout the range of the assay was evaluated in cell culture media.

Sample Type Average % Recovery Range %
Cell Culture Media (n=4) 101 92-111

Linearity

To assess the linearity of the assay, samples containing and/or spiked with high concentrations of Endoglin were serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.

Human Endoglin/CD105 ELISA Linearity

Scientific Data Images for Human Endoglin/CD105 Quantikine ELISA Kit

Human Endoglin/CD105 ELISA Standard Curve

Human Endoglin/CD105 ELISA Standard Curve

Preparation and Storage

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: Endoglin/CD105

Endoglin (CD105) is a transmembrane type III receptor for TGF-beta superfamily ligands and plays an important role in smooth muscle differentiation, angiogenesis, and neovascularization. It is highly expressed on proliferating vascular endothelial cells, chondrocytes, and syncytiotrophoblasts of term placenta. Human Endoglin haploinsufficiency can a cause the vascular disorder, hereditary hemorrhagic telangiectasia type I. Elevated levels of anti-angiogenic soluble Endoglin contribute to pathogenicity in preeclampsia. Endoglin associates with the receptors TGF-beta RII, Activin RIIA or RIIB, BMPR-IA/ALK-3, or BMPR-IB/ALK6 and enhance binding of Activin A, BMP-2, -7, -9, TGF-beta 1, or TGF-beta 3. Endoglin can either enhance or inhibit signaling through these receptor complexes.

Alternate Names

CD105, ENG

Entrez Gene IDs

2022 (Human); 13805 (Mouse); 497010 (Rat); 397096 (Porcine)

Gene Symbol

ENG

Additional Endoglin/CD105 Products

Product Documents for Human Endoglin/CD105 Quantikine ELISA Kit

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human Endoglin/CD105 Quantikine ELISA Kit

For research use only

⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Citations for Human Endoglin/CD105 Quantikine ELISA Kit

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  • Human Endoglin/CD105 Quantikine ELISA Kit
    Name: Anonymous
    Sample Tested: Plasma and Urine
    Verified Customer | Posted 11/08/2022
    Normal, healthy plasma was in range at 10-fold dilution and showed good linearity. Concentrations in normal, healthy urine were below the standard curve at 10-fold dilution so would recommend using a lower dilution factor.
    Human Endoglin/CD105 Quantikine ELISA Kit DNDG00

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Protocols

View specific protocols for Human Endoglin/CD105 Quantikine ELISA Kit (DNDG00):

Refer to the product for complete assay procedure.

Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
  1.   Prepare all reagents, standard dilutions, and samples as directed in the product insert.
  2.   Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.
    3. 100 µL Assay Diluent
  3.   Add 100 µL of Assay Diluent to each well.
    4. 50 µL Standard, Control, or Sample
  4.   Add 50 µL of Standard, control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours on a horizontal orbital microplate shaker.
  5.   Aspirate each well and wash, repeating the process 3 times for a total of 4 washes.
    6. 200 µL Conjugate
  6.   Add 200 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 2 hours on the shaker.
  7.   Aspirate and wash 4 times.
    8. 200 µL Substrate Solution
  8.   Add 200 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes on the benchtop. PROTECT FROM LIGHT.
    9. 50 µL Stop Solution
  9.   Add 50 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.

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FAQs for Human Endoglin/CD105 Quantikine ELISA Kit

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  • Q: Does this ELISA detect monomeric or dimeric Endoglin?

    A: This assay is confirmed to measure dimeric soluble Endoglin. This assay has not been evaluated for reactivity with monomeric Endoglin.

  • Q: Does this ELISA detect the L or S isoform of Endoglin?

    A: Both isoforms are expected to be measured in this Endoglin ELISA. This assay recognizes the soluble, extracellular portion of the protein which is identical for the L & S isoforms. The differences between L & S Endoglin are located within the cytoplasmic region of the protein.

  • Q: Does this ELISA detect monomeric or dimeric Endoglin?

    A: This assay is confirmed to measure dimeric soluble Endoglin. This assay has not been evaluated for reactivity with monomeric Endoglin.

  • Q: Does this ELISA detect the L or S isoform of Endoglin?

    A: Both isoforms are expected to be measured in this Endoglin ELISA. This assay recognizes the soluble, extracellular portion of the protein which is identical for the L & S isoforms. The differences between L & S Endoglin are located within the cytoplasmic region of the protein.

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