Key Product Details

Species Reactivity

Human

Applications

Flow Cytometry, CyTOF-ready

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # NP4D6
View all ENPP-3/CD203c Primary Antibodies »

Product Specifications

Immunogen

HEK293 human embryonic kidney cell line transfected with human ENPP‑3/CD203c
Accession # O14638

Specificity

Detects human ENPP‑3/CD203c.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human ENPP‑3/CD203c Antibody

Detection of ENPP-3/CD203c antibody in Human IL-3Ra+PBMCs antibody by Flow Cytometry.

Detection of ENPP‑3/CD203c in Human IL‑3R alpha +PBMCs by Flow Cytometry.

Human IL-3Ra+peripheral blood mononuclear cells were stained with Mouse Anti-Human ENPP-3/CD203c Monoclonal Antibody (Catalog # MAB5756) followed by Allophycocyanin-conjugated Anti-Mouse IgG F(ab')2Secondary Antibody (Catalog # F0101B) and Human IL-3 Ra Phycoerythrin-conjugated Monoclonal Antibody (Catalog # FAB301P).Quadrant markers were set based on control antibody staining (Catalog # MAB002).

Applications for Human ENPP‑3/CD203c Antibody

Application
Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

0.25 µg/106 cells
Sample: Human IL‑3R alpha + peripheral blood mononuclear cells

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Advanced Features

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  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: ENPP-3/CD203c

CD203c is a 150 kDa type II transmembrane glycoprotein with a short cytoplasmic tail. CD203c is also known as ectonucleotide pyrophosphatase/phosphodiesterase 3 (ENPP-3) and modulates purinergic signaling by hydrolysis of nucleotide triphosphates. CD203c is expressed on activated basophils and mast cells during allergic reactions, cardiac muscle, hepatocytes, and epithelial cells. It is upregulated in colon and bile duct carcinomas. Within the ECD, human CD203c shares 81% aa sequence identity with mouse and rat CD203.

Long Name

Ectonucleotide Pyrophosphatase/Phosphodiesterase 3

Alternate Names

B10, CD203c, ENPP3, NPP3, PD-IBETA, PDNP3

Entrez Gene IDs

5169 (Human)

Gene Symbol

ENPP3

UniProt

O14638

Additional ENPP-3/CD203c Products

Product Documents for Human ENPP‑3/CD203c Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human ENPP‑3/CD203c Antibody

For research use only

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