Human ERp57/PDIA3 Antibody

Catalog # Availability Size / Price Qty
AF8219
AF8219-SP
Detection of Human and Mouse ERp57/PDIA3 by Western Blot.
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Product Details
Citations (1)
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Human ERp57/PDIA3 Antibody Summary

Species Reactivity
Human
Specificity
Detects human ERp57/PDIA3 in direct ELISAs and Western blots.
Source
Polyclonal Sheep IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant ERp57/PDIA3
Arg107-Lys366
Accession # P30101
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.2 µg/mL
See below
Immunohistochemistry
5-15 µg/mL
See below
Immunocytochemistry
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Western Blot Detection of Human and Mouse ERp57/PDIA3 by Western Blot. View Larger

Detection of Human and Mouse ERp57/PDIA3 by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, HEK293 human embryonic kidney cell line, and Hepa 1‑6 mouse hepatoma cell line. PVDF membrane was probed with 0.2 µg/mL of Sheep Anti-Human ERp57/PDIA3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8219) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for ERp57/PDIA3 at approximately 57 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunocytochemistry ERp57/PDIA3 in HeLa Human Cell Line. View Larger

ERp57/PDIA3 in HeLa Human Cell Line. ERp57/PDIA3 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Sheep Anti-Human ERp57/PDIA3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8219) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Immunohistochemistry ERp57/PDIA3 in Human Liver. View Larger

ERp57/PDIA3 in Human Liver. ERp57/PDIA3 was detected in immersion fixed paraffin-embedded sections of human liver using Sheep Anti-Human ERp57/PDIA3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8219) at 1 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to the cytoplasm of hepatocytes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: ERp57/PDIA3

ERp57 (Endoplasmic reticulum resident protein 57; also Protein disulfide-isomerase A3/PDIA3) is a 57-60 kDa member of the protein disulfide isomerase family of proteins.  Knockdown of ERp57 or antibody-targeted inhibition of the secreted form significantly impaired the secretion and accumulation of extracellular matrix. ERp57 is highly similar to other PDI family members in amino acid sequence and structural/functional domain organization. This protein plays an important role in endoplasmic reticulum quality control of newly synthesized glycoproteins, is critical in major histocompatability complex class I assembly and regulates gene expression. ERp57 has been implicated in human pathologies including cancer and Alzheimer's disease. Within aa 107 - 366, human ERp57  shares 92% aa sequence identity with mouse ERp57.

Long Name
Endoplasmic Reticulum Resident Protein 57
Entrez Gene IDs
2923 (Human); 14827 (Mouse); 29468 (Rat)
Alternate Names
Disulfide isomerase ER-60; EC 5.3.4.1; endoplasmic reticulum P58; Endoplasmic reticulum resident protein 57; Endoplasmic reticulum resident protein 60; ER protein 60; ER60; ERp57; ERp5758 kDa glucose-regulated protein; ERp60; ERp6058 kDa microsomal protein; ERp61; glucose regulated protein, 58kDa; GRP57; GRP58; GRP58ER protein 57; HsT17083; P58; PDIA3; phospholipase C-alpha; PI-PLC; protein disulfide isomerase family A, member 3; protein disulfide isomerase-associated 3; protein disulfide-isomerase A3

Product Datasheets

Citation for Human ERp57/PDIA3 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Neuroprotective effects of vitamin D on high fat diet- and palmitic acid-induced enteric neuronal loss in mice
    Authors: S Larsson, U Voss
    BMC Gastroenterol, 2018;18(1):175.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: ICC

FAQs

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Cell and Tissue Staining Kits

Immunohistochemistry Reagents

Isotype Controls

Reconstitution Buffers

Secondary Antibodies

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