Human Erythropoietin R Antibody (713210)

R&D Systems | Catalog # MAB3073

R&D Systems

Key Product Details

Species Reactivity

Human

Applications

Neutralization

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 713210
View all Erythropoietin R Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Erythropoietin R
Ala25-Pro250
Accession # P19235

Specificity

Detects human Erythropoietin R in direct ELISAs.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Scientific Data Images for Human Erythropoietin R Antibody (713210)

Proliferation Induced by Erythropoietin and Neutralization by Human Erythropoietin R Antibody.

Proliferation Induced by Erythropoietin and Neutralization by Human Erythropoietin R Antibody.

Recombinant Human Erythropoietin (Tissue Culture Grade) (Catalog # 287-TC) induces proliferation in the TF-1 human erythroleukemic cell line in a dose-dependent manner (orange line), as measured by Resazurin (Catalog # AR002). Proliferation elicited by Recombinant Human Erythropoietin (Tissue Culture Grade) (0.278 units/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human Erythropoietin R Monoclonal Antibody (Catalog # MAB3073). The ND50 is typically 0.4-2.4 µg/mL.

Applications for Human Erythropoietin R Antibody (713210)

Application
Recommended Usage

Neutralization

Measured by its ability to neutralize Erythropoietin-induced proliferation in the TF‑1 human erythroleukemic cell line. The Neutralization Dose (ND50) is typically 0.4-2.4 µg/mL in the presence of 0.278 units/mL Recombinant Human Erythropoietin (Tissue Culture Grade).

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Erythropoietin R

Epo R is a transmembrane protein expressed on the surface of megakaryocytes, erythroid progenitors, and endothelial cells. It binds Epo and transmits signals that stimulate the proliferation and maturation of bone marrow erythroid precursors into red cells.

Long Name

Erythropoietin Receptor

Alternate Names

EpoR

Entrez Gene IDs

2057 (Human); 13857 (Mouse)

Gene Symbol

EPOR

UniProt

P19235

Additional Erythropoietin R Products

Product Documents for Human Erythropoietin R Antibody (713210)

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human Erythropoietin R Antibody (713210)

For research use only

Citations for Human Erythropoietin R Antibody (713210)

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Associated Pathways

Jak/STAT Signaling Pathway
Jak/STAT Signaling Pathway Thumbnail