Protein A or G purified from hybridoma culture supernatant
Mouse myeloma cell line NS0-derived recombinant human Erythropoietin R Ala25-Pro250 Accession # P19235
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
<0.10 EU per 1 μg of the antibody by the LAL method.
Measured by its ability to neutralize Erythropoietin-induced proliferation in the TF‑1 human erythroleukemic cell line. The Neutralization Dose (ND50) is typically 0.4-2.4 µg/mL in the presence of 0.278 units/mL Recombinant Human Erythropoietin (Tissue Culture Grade).
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Proliferation Induced by Erythropoietin and Neutralization by Human Erythropoietin R Antibody. Recombinant Human Erythropoietin (Tissue Culture Grade) (Catalog # 287-TC) induces proliferation in the TF‑1 human erythroleukemic cell line in a dose-dependent manner (orange line), as measured by Resazurin (Catalog # AR002). Proliferation elicited by Recombinant Human Erythropoietin (Tissue Culture Grade) (0.278 units/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human Erythropoietin R Monoclonal Antibody (Catalog # MAB3073). The ND50 is typically 0.4-2.4 µg/mL.
Preparation and Storage
Reconstitute at 0.5 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Erythropoietin R
Epo R is a transmembrane protein expressed on the surface of megakaryocytes, erythroid progenitors, and endothelial cells. It binds Epo and transmits signals that stimulate the proliferation and maturation of bone marrow erythroid precursors into red cells.
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