Human FCRL4/FcRH4 Antibody

Catalog #: MAB24262 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB24262
MAB24262-SP

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Detection of FCRL4/FcRH4 in RPMI 8226 Human Cell Line by Flow Cytometry.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human FCRL4/FcRH4 Antibody Summary

Species Reactivity

Human

Specificity

Detects human FCRL4/FcRH4 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human FCRL1 is observed.

Source

Monoclonal Mouse IgG₁ Clone # 580810

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant human FCRL4/FcRH4
Gln16-Asp385
Accession # NP_112572

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS and NaCl with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Flow Cytometry

2.5 µg/10⁶ cells

See below

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of FCRL4/FcRH4 in RPMI 8226 Human Cell Line by Flow Cytometry. RPMI 8226 human multiple myeloma cell line was stained with Mouse Anti-Human FCRL4/FcRH4 Monoclonal Antibody (Catalog # MAB24262, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B).

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Reconstitution Buffer Available

Reconstitution Buffer 1 (PBS)

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: FCRL4/FcRH4

Fc receptor-like 4 (FCRL4), also known as FcRH4 and IRTA1, is an Ig superfamily transmembrane protein that shares sequence homology with the classical Fc receptors. FCRL4 is preferentially expressed in B cells and contains three potential ITIM motifs in its cytoplasmic domain. The gene for FCRL4 is localized to the human chromosome 1q21‑23 region, a hotspot for translocation events.

Long Name

Fc Receptor-like protein 4

Entrez Gene IDs

83417 (Human)

Alternate Names

CD307d antigen; CD307d; Fc receptor homolog 4; Fc receptor-like 4; FcRH4; FCRH4Fc receptor-like protein 4; FCRL4; FcR-like protein 4; hIFGP2; IFGP family protein 2; IFGP2; IGFP2; Immune receptor translocation-associated protein 1; immunoglobulin superfamily Fc receptor, gp42; immunoglobulin superfamily receptor translocation associated 1; IRTA1; IRTA1MGC150523; MGC150522