Human FGF-19 Antibody

Catalog # Availability Size / Price Qty
AF969
AF969-SP
Detection  of Human FGF‑19 by Western Blot.
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Product Details
Citations (4)
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Human FGF-19 Antibody Summary

Species Reactivity
Human
Specificity
Detects human FGF-19 in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 1% cross‑reactivity with recombinant human (rh) FGF acidic, rhFGF basic, rhFGF-4, rhFGF-5, rhFGF-6, rhFGF-7, recombinant mouse (rm) FGF-8b, rmFGF-8c, rhFGF-9, rhFGF-10, rmFGF-15, rhFGF-17, and rhFGF-18 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human FGF-19
Phe27-Lys216
Accession # O95750
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.5 µg/mL
See below
Simple Western
10 µg/mL
See below
Blockade of Receptor-ligand Interaction
In a functional ELISA, 2-8 µg/mL of this antibody will block 50% of the binding of 250 ng/mL of Recombinant Human FGF-19 (Catalog # 969-FG) to immobilized Recombinant Human FGF R4 Fc Chimera (Catalog # 685-FR) coated at 2 µg/mL (100 µL/well). At 10 μg/mL, this antibody will block >90% of the binding.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Western Blot Detection  of Human FGF‑19 by Western Blot. View Larger

Detection of Human FGF‑19 by Western Blot. Western blot shows lysates of COLO 205 human colorectal adenocarcinoma cell line, conditioned media from COLO 205 cell line, and HT‑29 human colon adenocarcinoma cell line. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human FGF‑19 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF969) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for FGF‑19 at approximately 22 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Simple Western Detection of Human FGF‑19 by Simple WesternTM. View Larger

Detection of Human FGF‑19 by Simple WesternTM. Simple Western lane view shows conditioned media from COLO 205 human colorectal adenocarcinoma cell line and HT‑29 human colon adenocarcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for FGF‑19 at approximately 26 kDa (as indicated) using 10 µg/mL of Goat Anti-Human FGF‑19 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF969) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 2-40 kDa separation system.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: FGF-19

Fibroblast growth factor 19 (FGF-19) belongs to the large FGF family which has at least 23 members (1, 2). All FGF family members are heparin-binding growth factors with a core 120 amino acid (aa) FGF domain that allows for a common tertiary structure. FGFs are expressed during embryonic development and in restricted adult tissues. They act on cells of mesodermal and neuroectodermal origin to regulate diverse physiologic functions including angiogenesis, cell growth, pattern formation, embryonic development, metabolic regulation, cell migration, neurotrophic effects and tissue repair (3, 4). Signaling receptors for FGFs are type I transmembrane receptor tyrosine kinases belonging to the Ig superfamily. Four distinct but related classes of FGF receptors, FGF R1, 2, 3, and 4, exist. Through alternative splicing, multiple isoforms for FGF R1, 2 and 3, with distinct ligand recognition profiles, are also generated (4).

Human FGF-19 cDNA predicts a 251 aa precursor protein with a 22 aa signal peptide and a 229 aa secreted mature protein with no potential N-linked glycosylation sites (1, 2). Among FGF family members, human FGF-19 is most closely related to chicken FGF-19 and murine FGF-15, sharing approximately 61% and 51% aa sequence identity, respectively (1, 2, 5). Neither the human orthologue of mouse FGF-15, nor the mouse counterpart of human FGF-19 has been identified. With the exception of adult gall bladder epithelium, FGF-19 expression is restricted to fetal tissues (1, 2). Unlike most FGFs which bind to and activate more than one FGF receptor, FGF-19 is a specific ligand for FGF R4 (2). Similarly, another FGF family member, FGF-7 (KGF), only activates KGF R, the IIIb isoform of FGF R2 (4). During chick embryogenesis, FGF-19 has been shown to act synergistically with Wnt-8c to initiate inner ear development (5).

References
  1. Nishimura, T. et al. (1999) Biocheim. Biophys. Acta 1444:148.
  2. Xie, M. et al. (1999) Cytokine 11:729.
  3. Goldfarb, M. (1996) Cytokine & Growth Factor Reviews 7:311.
  4. Green, P. et al. (1996) BioEssays 18:639.
  5. Ladher, R.K. et al. (2000) Science 290:1965.
Long Name
Fibroblast Growth Factor 19
Entrez Gene IDs
9965 (Human)
Alternate Names
FGF19; FGF-19; fibroblast growth factor 19

Product Datasheets

Citations for Human FGF-19 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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  1. Characterization of a FGF19 variant with altered receptor specificity revealed a central role for FGFR1c in the regulation of glucose metabolism.
    Authors: Ge H, Baribault H, Vonderfecht S, Lemon B, Weiszmann J, Gardner J, Lee KJ, Gupte J, Mookherjee P, Wang M, Sheng J, Wu X, Li Y
    PLoS ONE, 2012;7(3):e33603.
    Species: Mouse
    Sample Types: Plasma
    Applications: ELISA Development
  2. FGF19 Regulates Cell Proliferation, Glucose and Bile Acid Metabolism via FGFR4-Dependent and Independent Pathways.
    Authors: Wu AL, Coulter S, Liddle C, Wong A, Eastham-Anderson J, French DM, Peterson AS, Sonoda J
    PLoS ONE, 2011;6(3):e17868.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: ELISA Development
  3. The hepatic response to FGF19 is impaired in patients with nonalcoholic fatty liver disease and insulin resistance.
    Authors: Schreuder TC, Marsman HA, Lenicek M
    Am. J. Physiol. Gastrointest. Liver Physiol., 2010;298(3):G440-5.
    Species: Human
    Sample Types: Plasma
    Applications: ELISA Development
  4. High expression of the bile salt-homeostatic hormone fibroblast growth factor 19 in the liver of patients with extrahepatic cholestasis.
    Authors: Schaap FG, van der Gaag NA, Gouma DJ
    Hepatology, 2009;49(4):1228-35.
    Species: Human
    Sample Types: Plasma
    Applications: ELISA Development

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