Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Western Blot, Immunocytochemistry

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2A Clone # 272317
View all FGF-20 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human FGF-20
Met1-Thr211
Accession # Q9NP95

Specificity

Detects human FGF-20 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross‑reactivity with recombinant human (rh) FGF-3, -4, -5, -6, -7, -9, -10, -11, -12, -16, -17, -18, -19, -23, recombinant mouse FGF-8b, -8c, -15, -23, rhFGF acidic, or rhFGF basic is observed.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2A

Scientific Data Images for Human FGF‑20 Antibody

FGF-20 antibody in SW480 Human Cell Line by Immunocytochemistry (ICC).

FGF‑20 in SW480 Human Cell Line.

FGF-20 was detected in immersion fixed SW480 human colorectal adenocarcinoma cell line using Rat Anti-Human FGF-20 Monoclonal Antibody (Catalog # MAB2547) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL013) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Applications for Human FGF‑20 Antibody

Application
Recommended Usage

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed SW480 human colorectal adenocarcinoma cell line

Western Blot

1 µg/mL
Sample: Recombinant Human FGF‑20 (Catalog # 2547-FG)

Reviewed Applications

Read 2 reviews rated 5 using MAB2547 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: FGF-20

Fibroblast growth factor 20 (FGF-20) is a member of the FGF gene family, which currently contains 22 members. Based on its structure, it is further classified as an FGF-9 subfamily member. All FGF family members are heparin-binding growth factors with a 120 amino acid (aa) core FGF domain that exhibits a beta -trefoil structure (1). The cDNA of FGF-20 predicts a 211 aa polypeptide without a canonical signal peptide sequence, a feature shared with other members of this subfamily (2‑4). Nevertheless, it is secreted with a molecular weight of 27 kDa (2‑4). FGF-20 is known to bind to heparin (4). No alternate splice forms have been reported. However, three amino acid polymorphisms are known, and single nucleotide polymorphisms in noncoding regions that may effect expression show a strong correlation with a risk of developing Parkinson’s disease (5, 6). Human FGF-20 shows 98% aa identity to bovine FGF-20 and 95% aa identity to both rat and mouse FGF-20. Within the FGF-9 subfamily, FGF-20 is 69% and 63% aa identical to human FGF-9 and FGF-16, respectively. Human FGF-20 is reported to be promiscuous in its selection of receptors which include FGF R1c, FGF R2c, FGF R3b, FGF R3c and FGF R4 (4, 7, 8). FGF-20 is expressed a variety of cells, including dopaminergic neurons (2), fibroblasts, keratinocytes and breast epithelium (4), and multiple sites in the fetus (2, 7). Finally, the expression of FGF-20 and DKK-1 is regulated by beta -catenin during development and tumorigenesis, implying that FGF-20 may play a role in the oncogenesis induced by the Wnt signaling pathway (9).

References

  1. Itoh, N. and D.M. Ornitz (2004) Trends Genet. 20:563.
  2. Ohmachi, S. et al. (2000) Biochem. Biophys. Res. Commun. 277:355.
  3. Kirikoshi, H. et al. (2000) Biochem. Biophys. Res. Commun. 274:337.
  4. Jeffers, M. et al. (2001) Cancer Res. 61:3131.
  5. van der Walt, J. et al. (2004) Am. J. Hum. Genet. 74:1121.
  6. Genbank Accession # Q9NP95.
  7. Lavine, K.J. et al. (2005) Dev. Cell 8:85.
  8. Ohmachi, S. et al. (2003) J. Neurosci. Res. 72:436.
  9. Chamorro, M.N. et al. (2005) EMBO J. 24:73.

Long Name

Fibroblast Growth Factor 20

Alternate Names

FGF20

Entrez Gene IDs

26281 (Human)

Gene Symbol

FGF20

UniProt

Q9NP95

Additional FGF-20 Products

Product Documents for Human FGF‑20 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human FGF‑20 Antibody

For research use only

Related Research Areas

FGF Family
FGF Ligands

Citations for Human FGF‑20 Antibody

Customer Reviews for Human FGF‑20 Antibody (2)

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  • Human FGF-20 Antibody
    Name: Anonymous
    Application: Immunocytochemistry/Immunofluorescence
    Sample Tested: SW480 human colorectal adenocarcinoma cell line
    Species: Human
    Verified Customer | Posted 03/25/2022
    Human FGF‑20 Antibody MAB2547
  • Human FGF-20 Antibody
    Name: Anonymous
    Application: ELISA
    Sample Tested: Purified Standard
    Species: Human
    Verified Customer | Posted 07/17/2017
    Sandwich ELISA. Tested against hFGF20 standards (800 pg/ml-25 pg/ml), run in duplicate.
    Human FGF‑20 Antibody MAB2547

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