Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Western Blot, Immunocytochemistry

Cited:

Immunohistochemistry, Western Blot, Flow Cytometry

Label

Unconjugated

Antibody Source

Polyclonal Sheep IgG
View all FOLR2 Primary Antibodies »

Product Specifications

Immunogen

Chinese hamster ovary cell line CHO-derived recombinant human FOLR2
Gln22-His228
Accession # P14207

Specificity

Detects human FOLR2 in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross-reactivity with recombinant human (rh) FOLR1 and rhFOLR3 is observed.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Scientific Data Images for Human FOLR2 Antibody

Detection of Human FOLR2 antibody by Western Blot.

Detection of Human FOLR2 by Western Blot.

Western blot shows lysates of human placental tissue. PVDF membrane was probed with 1 µg/mL of Human FOLR2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5697) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for FOLR2 at approximately 38 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

FOLR2 antibody in Human Neutrophils by Immunocytochemistry (ICC).

FOLR2 in Human Neutrophils.

FOLR2 was detected in immersion fixed human neutrophils using Human FOLR2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5697) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red, upper panel; Catalog # NL010) and counterstained with DAPI (blue, lower panel). Specific staining was localized to cell surfaces and cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Applications for Human FOLR2 Antibody

Application
Recommended Usage

Immunocytochemistry

5-15 µg/mL
Sample: Immersion fixed human neutrophils

Western Blot

1 µg/mL
Sample: Human placental tissue

Reviewed Applications

Read 1 review rated 3 using AF5697 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: FOLR2

Folate Receptor 2 (FOLR2), also known as Folate Receptor beta, is a 38 kDa protein that mediates the cellular uptake of folic acid and reduced folates. Dietary folates are required for many key metabolic processes including nucleotide and methionine synthesis, the interconversion of glycine and serine, and histidine breakdown (1, 2). Mature FOLR2 is an N-glycosylated protein that is anchored to the cell surface by a GPI linkage (3, 4). Human FOLR2 shares 83% amino acid sequence identity with mouse and rat FOLR2. FOLR2 is predominantly expressed in placenta, cells of the neutrophilic lineage, and some CD34+ hematopoietic progenitor cells (4‑6). It is upregulated on myeloid  leukemias, head and neck squamous cell carcinomas, and several nonepithelial cancers (3, 5, 7). It is also upregulated on macrophages and monocytes at chronic inflammatory sites including rheumatoid arthritis synovium and glioblastoma (8‑10). FOLR2 knockout mice do not show gross morphological defects, but they exhibit increased sensitivity to arsenate-induced teratogenicity (11, 12).

References

  1. Fowler, B. et al. (2001) Kidney Int. 59:S-221.
  2. Kelemen, L.E. (2006) Int. J. Cancer 119:243.
  3. Shen, F. et al. (1994) Biochemistry 33:1209.
  4. Ratnam, M. et al. (1989) Biochemistry 28:8249.
  5. Ross, J.F. et al. (1999) Cancer 85:348.
  6. Reddy, J.A. et al. (1999) Blood 93:3940.
  7. Ross, J.F. et al. (1994) Cancer 73:2432.
  8. Nakashima-Matsushita, N. et al. (1999) Arthritis Rheum. 42:1609.
  9. van der Heijden, J.W. et al. (2009) Arthritis Rheum. 60:12.
  10. Nagai, T. et al. (2009) Cancer Immunol. Immunother. Feb 24 epub.
  11. Piedrahita, J.A. et al. (1999) Nat. Genet. 23:228.
  12. Wlodarczyk, B. et al. (2001) Toxicol. Appl. Pharmacol. 177:238.

Long Name

Folate Receptor 2

Alternate Names

beta-HFR, FR-beta, FR-P3

Entrez Gene IDs

2350 (Human); 14276 (Mouse); 293154 (Rat)

Gene Symbol

FOLR2

UniProt

P14207 (Human)

Additional FOLR2 Products

Product Documents for Human FOLR2 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human FOLR2 Antibody

For research use only

Related Research Areas

Cancer Biomarkers

Citations for Human FOLR2 Antibody

Customer Reviews for Human FOLR2 Antibody (1)

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  • Human FOLR2 Antibody
    Name: Anonymous
    Application: Immunocytochemistry/Immunofluorescence
    Sample Tested: Melanoma tissue
    Species: Human
    Verified Customer | Posted 11/04/2021
    Human FOLR2 Antibody AF5697

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Protocols

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