Human FOLR2 Antibody

(1 citations)   
  • Species Reactivity
    Human
  • Specificity
    Detects human FOLR2 in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross-reactivity with recombinant human (rh) FOLR1 and rhFOLR3 is observed.
  • Source
    Polyclonal Sheep IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    Chinese hamster ovary cell line CHO-derived recombinant human FOLR2
    Gln22-His228
    Accession # P14207
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1 µg/mL
    See below
  • Immunocytochemistry
    5-15 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Human FOLR2 by Western Blot. Western blot shows lysates of human placental tissue. PVDF membrane was probed with 1 µg/mL of Human FOLR2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5697) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for FOLR2 at approximately 38 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
Immunocytochemistry
FOLR2 in Human Neutrophils. FOLR2 was detected in immersion fixed human neutrophils using Human FOLR2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5697) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red, upper panel; Catalog # NL010) and counterstained with DAPI (blue, lower panel). Specific staining was localized to cell surfaces and cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: FOLR2
Folate Receptor 2 (FOLR2), also known as Folate Receptor beta, is a 38 kDa protein that mediates the cellular uptake of folic acid and reduced folates. Dietary folates are required for many key metabolic processes including nucleotide and methionine synthesis, the interconversion of glycine and serine, and histidine breakdown (1, 2). Mature FOLR2 is an N-glycosylated protein that is anchored to the cell surface by a GPI linkage (3, 4). Human FOLR2 shares 83% amino acid sequence identity with mouse and rat FOLR2. FOLR2 is predominantly expressed in placenta, cells of the neutrophilic lineage, and some CD34+ hematopoietic progenitor cells (4‑6). It is upregulated on myeloid  leukemias, head and neck squamous cell carcinomas, and several nonepithelial cancers (3, 5, 7). It is also upregulated on macrophages and monocytes at chronic inflammatory sites including rheumatoid arthritis synovium and glioblastoma (8‑10). FOLR2 knockout mice do not show gross morphological defects, but they exhibit increased sensitivity to arsenate-induced teratogenicity (11, 12).
  • References:
    1. Fowler, B. et al. (2001) Kidney Int. 59:S-221.
    2. Kelemen, L.E. (2006) Int. J. Cancer 119:243.
    3. Shen, F. et al. (1994) Biochemistry 33:1209.
    4. Ratnam, M. et al. (1989) Biochemistry 28:8249.
    5. Ross, J.F. et al. (1999) Cancer 85:348.
    6. Reddy, J.A. et al. (1999) Blood 93:3940.
    7. Ross, J.F. et al. (1994) Cancer 73:2432.
    8. Nakashima-Matsushita, N. et al. (1999) Arthritis Rheum. 42:1609.
    9. van der Heijden, J.W. et al. (2009) Arthritis Rheum. 60:12.
    10. Nagai, T. et al. (2009) Cancer Immunol. Immunother. Feb 24 epub.
    11. Piedrahita, J.A. et al. (1999) Nat. Genet. 23:228.
    12. Wlodarczyk, B. et al. (2001) Toxicol. Appl. Pharmacol. 177:238.
  • Long Name:
    Folate Receptor 2
  • Entrez Gene IDs:
    2350 (Human); 14276 (Mouse); 293154 (Rat)
  • Alternate Names:
    beta-HFR; FBP; FBP/PL-1; folate receptor 2 (fetal); Folate receptor 2; folate receptor beta; folate receptor, beta; Folate receptor, fetal/placental; folate-binding protein, fetal/placental; FOLR2; FR-beta; FR-P3; Placental folate-binding protein
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citations: Showing 1 - 1

  1. Glucocorticoids enhance CD163 expression in placental Hofbauer cells.
    Authors: Tang Z, Niven-Fairchild T, Tadesse S, Norwitz E, Buhimschi C, Buhimschi I, Guller S
    Endocrinology, 2013;154(1):471-82.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
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