Key Product Details

Species Reactivity

Human

Applications

Western Blot, Immunocytochemistry

Label

Unconjugated

Antibody Source

Polyclonal Sheep IgG
View all FoxD3 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human FoxD3
Met1-Val140
Accession # Q9UJU5

Specificity

Detects human FoxD3 (aa 1-140) in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human (rh) FoxP3, rhFoxP1, and rhFoxO3A is observed.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Scientific Data Images for Human FoxD3 aa 1‑140 Antibody

Detection of Human FoxD3 antibody by Western Blot.

Detection of Human FoxD3 by Western Blot.

Western blot shows lysates of HEK293 human embryonic kidney cell line either mock transfected or transfected with human FoxD3. PVDF Membrane was probed with 1 µg/mL of Sheep Anti-Human FoxD3 aa 1-140 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2819) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for FoxD3 at approximately 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
FoxD3 antibody in BG01V Human Stem Cells by Immunocytochemistry (ICC).

FoxD3 in BG01V Human Stem Cells.

FoxD3 was detected in immersion fixed BG01V human embryonic stem cells using Sheep Anti-Human FoxD3 aa 1-140 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2819) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red, upper panel; Catalog # NL010) and counterstained with DAPI (blue, lower panel). Specific staining was localized to nuclei and cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Applications for Human FoxD3 aa 1‑140 Antibody

Application
Recommended Usage

Immunocytochemistry

5-15 µg/mL
Sample: Immersion fixed BG01V human embryonic stem cells

Western Blot

1 µg/mL
Sample: HEK293 human embryonic kidney cell line transfected with human FoxD3

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: FoxD3

FOXD3 (forkhead box protein D3; also HNF3, HFH2 and Genesis) is a 58-60 kDa member of the winged helix transcription factor gene family. It is expressed in the epiblast, migrating neural crest, and neural progenitors of the developing spinal cord. FOXD3 is believed to maintain an uncommitted state in multipotent neural crest stem cells, and to induce cell cycle arrest via p53 and p21cip1. Human FOXD3 is 478 amino acids (aa) in length. It contains a forkhead DNA binding domain (aa 141‑235) plus an Ala-rich region (aa 262-473). Over aa 1-140 and 302-462, human FOXD3 shares 76% and 91% aa identity with mouse FoxD3, respectively.

Long Name

Forkhead Box D3

Alternate Names

CWH3, Genesis, HFH2

Entrez Gene IDs

27022 (Human); 15221 (Mouse)

Gene Symbol

FOXD3

UniProt

Q9UJU5

Additional FoxD3 Products

Product Documents for Human FoxD3 aa 1‑140 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human FoxD3 aa 1‑140 Antibody

For research use only

Citations for Human FoxD3 aa 1‑140 Antibody

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Protocols

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