FOXD3 (forkhead box protein D3; also HNF3, HFH2 and Genesis) is a 58-60 kDa member of the winged helix transcription factor gene family. It is expressed in the epiblast, migrating neural crest, and neural progenitors of the developing spinal cord. FOXD3 is believed to maintain an uncommitted state in multipotent neural crest stem cells, and to induce cell cycle arrest via p53 and p21cip1. Human FOXD3 is 478 amino acids (aa) in length. It contains a forkhead DNA binding domain (aa 141‑235) plus an Ala-rich region (aa 262-473). Over aa 1-140 and 302-462, human FOXD3 shares 76% and 91% aa identity with mouse FoxD3, respectively.
Human FoxD3 aa 1-140 Antibody
R&D Systems | Catalog # AF2819
Scientific images may be resized, cropped, or adjusted for brightness or contrast for presentation purposes. These updates are limited to presentation and do not affect the underlying scientific interpretation of the data. Where available, additional source data may be provided upon request.
Key Product Details
Species Reactivity
Human
Applications
Western Blot, Immunocytochemistry
Label
Unconjugated
Antibody Source
Polyclonal Sheep IgG
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Product Specifications
Immunogen
E. coli-derived recombinant human FoxD3
Met1-Val140
Accession # Q9UJU5
Met1-Val140
Accession # Q9UJU5
Specificity
Detects human FoxD3 (aa 1-140) in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human (rh) FoxP3, rhFoxP1, and rhFoxO3A is observed.
Clonality
Polyclonal
Host
Sheep
Isotype
IgG
Scientific Data Images for Human FoxD3 aa 1-140 Antibody
Detection of Human FoxD3 by Western Blot.
Western blot shows lysates of HEK293 human embryonic kidney cell line either mock transfected or transfected with human FoxD3. PVDF Membrane was probed with 1 µg/mL of Sheep Anti-Human FoxD3 aa 1-140 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2819) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for FoxD3 at approximately 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
FoxD3 in BG01V Human Stem Cells.
FoxD3 was detected in immersion fixed BG01V human embryonic stem cells using Sheep Anti-Human FoxD3 aa 1-140 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2819) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red, upper panel; Catalog # NL010) and counterstained with DAPI (blue, lower panel). Specific staining was localized to nuclei and cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.Applications for Human FoxD3 aa 1-140 Antibody
Application
Recommended Usage
Immunocytochemistry
5-15 µg/mL
Sample: Immersion fixed BG01V human embryonic stem cells
Sample: Immersion fixed BG01V human embryonic stem cells
Western Blot
1 µg/mL
Sample: HEK293 human embryonic kidney cell line transfected with human FoxD3
Sample: HEK293 human embryonic kidney cell line transfected with human FoxD3
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
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Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Calculators
Background: FoxD3
Long Name
Forkhead Box D3
Alternate Names
CWH3, Genesis, HFH2
Gene Symbol
FOXD3
UniProt
Additional FoxD3 Products
Product Documents for Human FoxD3 aa 1-140 Antibody
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Human FoxD3 aa 1-140 Antibody
For research use only
Related Research Areas
Citations for Human FoxD3 aa 1-140 Antibody
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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Associated Pathways
