Human FoxM1 Antibody

  
  • Species Reactivity
    Human
  • Specificity
    Detects human FoxM1 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human (rh) FoxB2 and rhFoxJ1 is observed.
  • Source
    Polyclonal Sheep IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    E. coli-derived recombinant human FoxM1
    Thr642-Gln763
    Accession # Q08050
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1 µg/mL
    See below
  • Immunocytochemistry
    5-15 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Immunocytochemistry
FoxM1 in U2OS Human Cell Line. FoxM1 was detected in immersion fixed U2OS human osteosarcoma cell line using 10 µg/mL Sheep Anti-Human FoxM1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3975) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained (green). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Detection of Human FoxM1 by Western Blot. Western blot shows lysates of U2OS human osteosarcoma cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human FoxM1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3975) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for FoxM1 at approximately 100 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: FoxM1

FoxM1 (Forkhead box protein M1; also FKHL16, HFH11 and WIN) is a 90-100 kDa member of the winged helix transcription factor gene family. It is expressed in proliferating and tumor cells and promotes the transition from G1 to S phase and G2 to M phase within the cell cycle. Human FoxM1 is 763 amino acids (aa) in length. It contains three PEST sequences (aa 22-34, 460-472 and 478-515), a forkhead DNA binding domain (aa 235-327), a Pro/Ser/Thr-rich region (aa 480-513) and a phosphorylation site at Ser704. There are at least two splice variants. The first shows a 38 aa insertion after Val423. This blocks FoxM1 transcriptional activation activity. The second variant shows a deletion of aa 326-340. Over aa 642-763, human FoxM1 shares 73% aa identity with mouse FoxM1.

  • Long Name:
    Forkhead Box M1
  • Entrez Gene IDs:
    2305 (Human); 14235 (Mouse); 58921 (Rat)
  • Alternate Names:
    FKHL16; FKHL16trident; forkhead box M1; forkhead box protein M1; Forkhead, drosophila, homolog-like 16; forkhead-like 16; Forkhead-related protein FKHL16; FoxM1; Hepatocyte nuclear factor 3 forkhead homolog 11; HFH-11; HFH11FOXM1B; HFH-11M-phase phosphoprotein 2; HNF-3; HNF-3/fork-head homolog 11; INS-1; MPHOSPH2; MPP-2; MPP2MPM-2 reactive phosphoprotein 2; PIG29; TGT3; Transcription factor Trident; Trident; WIN; Winged-helix factor from INS-1 cells
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