Human FoxM1 Antibody

Catalog # Availability Size / Price Qty
AF3975
AF3975-SP
FoxM1 in U2OS Human Cell Line.
8 Images
Product Details
Citations (2)
FAQs
Supplemental Products
Reviews

Human FoxM1 Antibody Summary

Species Reactivity
Human
Specificity
Detects human FoxM1 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human (rh) FoxB2 and rhFoxJ1 is observed.
Source
Polyclonal Sheep IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human FoxM1
Thr642-Gln763
Accession # Q08050
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below
Immunocytochemistry
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunocytochemistry FoxM1 antibody in U2OS Human Cell Line by Immunocytochemistry (ICC). View Larger

FoxM1 in U2OS Human Cell Line. FoxM1 was detected in immersion fixed U2OS human osteosarcoma cell line using 10 µg/mL Sheep Anti-Human FoxM1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3975) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained (green). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Western Blot Detection of Human FoxM1 antibody by Western Blot. View Larger

Detection of Human FoxM1 by Western Blot. Western blot shows lysates of U2OS human osteosarcoma cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human FoxM1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3975) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for FoxM1 at approximately 100 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Western Blot Detection of Human FoxM1 by Western Blot View Larger

Detection of Human FoxM1 by Western Blot RASSF1A regulation is dependent on FoxM1. T84 and Colo 205 cells were transfected with plasmid for overexpression of FoxM1. (A) Cell lysates were analyzed by immunoblot and quantified by densitometry for expression of FoxM1, RASSF1A. Expression is normalized against GAPDH. The right panel of (A) represents the densitometric analysis of FoxM1 and RASSF1A. (B) T84 and Colo 205 cells were transfected with siRNA for FoxM1 or control siRNA for 48 h. Cell lysates were evaluated for FoxM1 ((B), 1st lane), RASSF1A ((B), 2nd lane) by immunoblot and quantified by densitometry. The results are from three independent experiments. (** p < 0.01, *** p < 0.001). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/30744076), licensed under a CC-BY license. Not internally tested by R&D Systems.

Western Blot Detection of Human FoxM1 by Western Blot View Larger

Detection of Human FoxM1 by Western Blot Neutralizing VEGF receptor antibodies and Akt inhibition upregulates RASSF1A and downregulates FoxM1 in mCRC. T84 and Colo 205 cells were treated with VEGF receptor 1 and VEGFR2 as indicated before (see Section 2.3). Protein expression from cell lysates was determined by immunoblotting for RASSF1A ((A), upper panel) and FoxM1 ((A), middle panel). Densitometry analysis was performed and normalyzed with GAPDH expression to demonstrate significant upregulation for RASSF1A (B) and downregulation of FoxM1 in the presence of neutralized (NT) VEGFR1 or VEGFR2 (C). (D,E) T84 and Colo 205 cells were treated with Akt inhibitor (wortmannin) with different doses (0–1 µM) for 24 h. Cell lysates were analyzed for pAkt (1st lane), total Akt (2nd lane), RASSF1A (3rd lane) expression by immunoblot analysis and quantified by densitometry (F,G). The results are from three independent experiments. (* p < 0.05, ** p < 0.01, *** p < 0.001). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/30744076), licensed under a CC-BY license. Not internally tested by R&D Systems.

Western Blot Detection of Human FoxM1 by Western Blot View Larger

Detection of Human FoxM1 by Western Blot RASSF1A regulation is dependent on FoxM1. T84 and Colo 205 cells were transfected with plasmid for overexpression of FoxM1. (A) Cell lysates were analyzed by immunoblot and quantified by densitometry for expression of FoxM1, RASSF1A. Expression is normalized against GAPDH. The right panel of (A) represents the densitometric analysis of FoxM1 and RASSF1A. (B) T84 and Colo 205 cells were transfected with siRNA for FoxM1 or control siRNA for 48 h. Cell lysates were evaluated for FoxM1 ((B), 1st lane), RASSF1A ((B), 2nd lane) by immunoblot and quantified by densitometry. The results are from three independent experiments. (** p < 0.01, *** p < 0.001). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/30744076), licensed under a CC-BY license. Not internally tested by R&D Systems.

Western Blot Detection of Human FoxM1 by Western Blot View Larger

Detection of Human FoxM1 by Western Blot FoxM1 and RASSF1A expression is inversely co-related in colon cancer tissues. (A) Tissue extranction from both normal and colon cancer patient for monitoring the translational level of FoxM1 and RASSF1A by immunoblot. Quantification of RASSF1A (B) and FoxM1 (C) by scanning densitometry. GAPDH was used as a loading control. (D) T84 and Colo 205 cells were treated with or without AGP IC50 (45 µM) for 48 h. Cell lysates were analyzed by Western blot for FoxM1 and GAPDH expression. (E) Quantitative estimations of FoxM1 levels determined by densitometry measurements of western blots from three independent experiments after normalization with GAPDH (p < 0.001). T84 and Colo 205 cells were treated with or without AGP as indicated before and the transcriptional level were determined by qRT-PCR for (F) FoxM1 and (G) PTTG1. Bar graphs show quantitative results normalized to GAPDH mRNA levels. Results are from three independent experiments and statistical significance was determined using one way-ANOVA followed Bonferroni test. (* p < 0.05, ** p < 0.01, *** p < 0.001). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/30744076), licensed under a CC-BY license. Not internally tested by R&D Systems.

Western Blot Detection of Human FoxM1 by Western Blot View Larger

Detection of Human FoxM1 by Western Blot FoxM1 inhibitor induced RASSF1A and PTEN expression and YAP phosphorylation in mCRC cells. T84 and Colo 205 cells were treated with FoxM1 inhibitor, thiostrepton (Th) with different doses (0–8 µM) for 48 h. (A,B) mRNA was extracted after 24 h for detection of RASSF1A by qRT-PCR. Bar graphs show quantitative results normalized to GAPDH mRNA levels. Cell lysates were monitored by immunoblot for FoxM1(C), RASSF1A (D), p-YAP and total YAP (E), PTEN (F), and GAPDH as indicated. Immunoblots were quantified by scanning densitometry and normalized against GAPDH expression (lower panels of (C–E) for FoxM1, RASSF1A and p-YAP, respectively. Right panel of (F) represents the quantification of PTEN). (G) Patient derived organoids were treated with (8 µM) and without thiostrepton for 24 h and 48 h. Organoid cell lysates were analyzed by immunoblot for RASSF1A expression and quantified by densitometry ((G), lower panel). The results are from three independent experiments. (* p < 0.05, ** p < 0.01, *** p < 0.001). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/30744076), licensed under a CC-BY license. Not internally tested by R&D Systems.

Western Blot Detection of Human FoxM1 by Western Blot View Larger

Detection of Human FoxM1 by Western Blot FoxM1 and RASSF1A expression is inversely co-related in colon cancer tissues. (A) Tissue extranction from both normal and colon cancer patient for monitoring the translational level of FoxM1 and RASSF1A by immunoblot. Quantification of RASSF1A (B) and FoxM1 (C) by scanning densitometry. GAPDH was used as a loading control. (D) T84 and Colo 205 cells were treated with or without AGP IC50 (45 µM) for 48 h. Cell lysates were analyzed by Western blot for FoxM1 and GAPDH expression. (E) Quantitative estimations of FoxM1 levels determined by densitometry measurements of western blots from three independent experiments after normalization with GAPDH (p < 0.001). T84 and Colo 205 cells were treated with or without AGP as indicated before and the transcriptional level were determined by qRT-PCR for (F) FoxM1 and (G) PTTG1. Bar graphs show quantitative results normalized to GAPDH mRNA levels. Results are from three independent experiments and statistical significance was determined using one way-ANOVA followed Bonferroni test. (* p < 0.05, ** p < 0.01, *** p < 0.001). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/30744076), licensed under a CC-BY license. Not internally tested by R&D Systems.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Loading...
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: FoxM1

FoxM1 (Forkhead box protein M1; also FKHL16, HFH11 and WIN) is a 90-100 kDa member of the winged helix transcription factor gene family. It is expressed in proliferating and tumor cells and promotes the transition from G1 to S phase and G2 to M phase within the cell cycle. Human FoxM1 is 763 amino acids (aa) in length. It contains three PEST sequences (aa 22-34, 460-472 and 478-515), a forkhead DNA binding domain (aa 235-327), a Pro/Ser/Thr-rich region (aa 480-513) and a phosphorylation site at Ser704. There are at least two splice variants. The first shows a 38 aa insertion after Val423. This blocks FoxM1 transcriptional activation activity. The second variant shows a deletion of aa 326-340. Over aa 642-763, human FoxM1 shares 73% aa identity with mouse FoxM1.

Long Name
Forkhead Box M1
Entrez Gene IDs
2305 (Human); 14235 (Mouse); 58921 (Rat)
Alternate Names
FKHL16; FKHL16trident; forkhead box M1; forkhead box protein M1; Forkhead, drosophila, homolog-like 16; forkhead-like 16; Forkhead-related protein FKHL16; FoxM1; Hepatocyte nuclear factor 3 forkhead homolog 11; HFH-11; HFH11FOXM1B; HFH-11M-phase phosphoprotein 2; HNF-3; HNF-3/fork-head homolog 11; INS-1; MPHOSPH2; MPP-2; MPP2MPM-2 reactive phosphoprotein 2; PIG29; TGT3; Transcription factor Trident; Trident; WIN; Winged-helix factor from INS-1 cells

Product Datasheets

You must select a language.

x

Citations for Human FoxM1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
Filter your results:

Filter by:

  1. Identification of Cross Talk between FoxM1 and RASSF1A as a Therapeutic Target of Colon Cancer
    Authors: Thomas G. Blanchard, Steven J. Czinn, Vivekjyoti Banerjee, Neha Sharda, Andrea C. Bafford, Fahad Mubariz et al.
    Cancers (Basel)
  2. PRRX1 isoforms cooperate with FOXM1 to regulate the DNA damage response in pancreatic cancer cells
    Authors: B Marchand, JR Pitarresi, M Reichert, K Suzuki, D Laczkó, AK Rustgi
    Oncogene, 2019-01-31;0(0):.

FAQs

No product specific FAQs exist for this product, however you may

View all Antibody FAQs
Loading...

Reviews for Human FoxM1 Antibody

There are currently no reviews for this product. Be the first to review Human FoxM1 Antibody and earn rewards!

Have you used Human FoxM1 Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review