Human Galectin-3 Biotinylated Antibody

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Product Details
Citations (9)
Supplemental Products
Reviews (2)

Human Galectin-3 Biotinylated Antibody Summary

Species Reactivity
Detects human Galectin‑3 in ELISAs and Western blots. In sandwich ELISAs, approximately 25% cross-reactivity with recombinant mouse Galectin‑3 is observed and less than 0.2% cross-reactivity with recombinant human Galectin‑1, -2, -4, -7, -8, and -10 is observed.
Polyclonal Goat IgG
Antigen Affinity-purified
E. coli-derived recombinant human Galectin‑3
Accession # Q6IBA7
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.


Recommended Concentration
Western Blot
0.1 µg/mL
Recombinant Human Galectin‑3 (Catalog # 1154-GA)
Flow Cytometry
2.5 µg/106 cells
Human PBMCs fixed with paraformaldehyde and permeabilized with saponin

Human Galectin-3 Sandwich Immunoassay

Recommended Concentration
ELISA Detection (Matched Antibody Pair)
0.1-0.4 µg/mL 

Use in combination with:

Capture Reagent: Human Galectin‑3 Antibody (Catalog # MAB11541)

Standard: Recombinant Human Galectin-3 Protein (Catalog # 1154-GA)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

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Preparation and Storage

Reconstitute at 0.2 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Galectin-3

The galectins constitute a large family of carbohydrate-binding proteins with specificity for N-acetyl-lactosamine-containing glycoproteins. At least 14 mammalian galectins, which share structural similarities in their carbohydrate recognition domains (CRD), have been identified. The galectins have been classified into the prototype galectins (-1, -2, -5, -7, -10, -11, -13, -14), which contain one CRD and exist either as a monomer or a noncovalent homodimer; the chimera galectins (Galectin-3) containing one CRD linked to a nonlectin domain; and the tandem-repeat galectins (-4, -6, -8, -9, -12) consisting of two CRDs joined by a linker peptide. Galectins lack a classical signal peptide and can be localized to the cytosolic compartments where they have intracellular functions. However, via one or more as yet unidentified non-classical secretory pathways, galectins can also be secreted to function extracellularly. Individual members of the galectin family have different tissue distribution profiles and exhibit subtle differences in their carbohydrate-binding specificities. Each family member may preferentially bind to a unique subset of cell-surface glycoproteins (1-4).

Galectin-3, also known as Mac-2, L29, CBP35, and epsilon BP, is a chimera galectin that has a tendency to dimerize. Besides the soluble protein, alternatively spliced forms of chicken Galectin-3 containing a transmembrane-spanning domain and a leucine zipper motif have been reported. Galectin-3 is expressed in tumor cells, macrophages, activated T cells, osteoclasts, epithelial cells, and fibroblasts. It binds various matrix glycoproteins including laminin, fibronectin, LAMPS, 90K/Mac-2BP, MP20, and CEA. Galectin-3 promotes cell growth and proliferation for many cell types. Galectin-3 acts intracellularly to prevent apoptosis. Depending on the cell types, Galectin-3 exhibits pro- or anti-adhesive properties. Galectin-3 has proinflammatory activities in vitro and in vivo. It induces pro-inflammatory and inhibits Th2 type cytokine production. Galectin-3 chemoattracts monocytes and macrophages. It activates and degranulates basophils and mast cells. Elevated circulating levels of Galectin-3 has been show to correlate with the malignant potential of several types of cancer, suggesting that Galectin-3 is also involved in tumor growth and metastasis. Human and mouse Galectin-3 shares approximately 80% amino acid sequence similarity (1-5).

  1. Rabinovich, A. et al. (2002) Trends in Immunol. 23:313.
  2. Rabinovich, A. et al. (2002) J. Leukocyte Biology 71:741.
  3. Hughes, R.C. (2001) Biochimie 83:667.
  4. R&D Systems Cytokine Bulletin; Summer 2002.
  5. Gorski, J.P. et al. (2002) J. Biol. Chem. 277:18840.
Entrez Gene IDs
3958 (Human); 16854 (Mouse)
Alternate Names
AGE-R3; Carbohydrate-binding protein 35; CBP 35,35 kDa lectin; CBP35; GAL3; Gal-3; galactin-3; Galactose-specific lectin 3; Galactoside-binding protein; GALBPCBP35; Galectin3; Galectin-3; GALIG; IgE-binding protein; L29; L31; L-31; Laminin-binding protein; Lectin L-29; lectin, galactoside-binding, soluble, 3; LGALS2; LGALS3; Mac-2 antigen; Mac-2; MAC2GAL3

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Citations for Human Galectin-3 Biotinylated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

9 Citations: Showing 1 - 9
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  1. Chemically modified, non-anticoagulant heparin derivatives are potent galectin-3 binding inhibitors and inhibit circulating galectin-3-promoted metastasis
    Authors: Carrie A. Duckworth, Scott E. Guimond, Paulina Sindrewicz, Ashley J. Hughes, Neil S. French, Lu-Yun Lian et al.
  2. Sequence‐Defined Heteromultivalent Precision Glycomacromolecules Bearing Sulfonated/Sulfated Nonglycosidic Moieties Preferentially Bind Galectin‐3 and Delay Wound Healing of a Galectin‐3 Positive Tumor Cell Line in an In Vitro Wound Scratch Assay
    Authors: Tanja Freichel, Viktoria Heine, Dominic Laaf, Eleanor E. Mackintosh, Sophia Sarafova, Lothar Elling et al.
    Macromolecular Bioscience
  3. C1GalT1 expression reciprocally controls tumour cell-cell and tumour-macrophage interactions mediated by galectin-3 and MGL with double impact on cancer development and progression
    Authors: Wan, Y;Adair, K;Herrmann, A;Shan, X;Xia, L;Duckworth, CA;Yu, LG;
    Cell death & disease
    Species: Human
    Sample Types: Cell Lysates
    Applications: Immunoprecipitation
  4. Distinct features of the host-parasite interactions between nonadherent and adherent Trichomonas vaginalis isolates
    Authors: HM Hsu, YY Yang, YH Huang, CH Chu, TJ Tu, YT Wu, CJ Chiang, SB Yang, DK Hsu, FT Liu, JH Tai
    PloS Neglected Tropical Diseases, 2023-01-03;17(1):e0011016.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: ELISA Detection
  5. Galectin-3 deficiency in pregnancy increases the risk of fetal growth restriction (FGR) via placental insufficiency
    Authors: N Freitag, I Tirado-Gon, G Barrientos, KL Powell, P Boehm-Stur, SP Koch, K Hecher, AC Staff, PC Arck, A Diemert, SM Blois
    Cell Death Dis, 2020-07-23;11(7):560.
    Species: Human
    Sample Types: Serum
    Applications: ELISA Detection
  6. Endoglin Protein Interactome Profiling Identifies TRIM21 and Galectin-3 as New Binding Partners
    Authors: E Gallardo-V, L Ruiz-Llore, J Casado-Vel, MJ Ruiz-Rodrí, N López-Andr, AK Pattnaik, M Quintanill, C Bernabeu
    Cells, 2019-09-13;8(9):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  7. Mesenchymal stem cell-mediated T cell suppression occurs through secreted galectins.
    Authors: Sioud M, Mobergslien A, Boudabous A, Floisand Y
    Int. J. Oncol., 2010-12-09;38(2):385-90.
    Species: Human
    Sample Types: Cell Lysates, Whole Cells
    Applications: Flow Cytometry, Western Blot
  8. Galectin-Levels Are Elevated in Infants Born Preterm Due to Amniotic Infection and Rapidly Decline in the Neonatal Period
    Authors: Kirstin Faust, Nancy Freitag, Gabriela Barrientos, Christoph Hartel, Sandra M. Blois
    Frontiers in Immunology
  9. Galectin-3 Is a Natural Binding Ligand of MCAM (CD146, MUC18) in Melanoma Cells and Their Interaction Promotes Melanoma Progression
    Authors: Yaoyu Pang, Ellen Maxwell, Paulina Sindrewicz-Goral, Andrew Shapanis, Shun Li, Mark Morgan et al.


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Human Galectin‑3 Biotinylated Antibody
By Anonymous on 10/26/2015
Application: WB Sample Tested: Cancer cell lysates Species: Human

Specificity: Specific
Sensitivity: Sensitive
Buffer: Milk BSA Milk
Dilution: 1:1000

Human Galectin‑3 Biotinylated Antibody
By Anonymous on 10/26/2015
Application: IHC Sample Tested: Colon tissue Species: Human

This antibody was first tested on tissues before the mouse IgG monoclonal version, the staining is diffuse and not as good as the other clone.
Specificity: Reasonably specific
Sensitivity: Not sensitive
Buffer: PBS
Dilution: 1:500