Human Gastrokine 1 Antibody

  
  • Species Reactivity
    Human
  • Specificity
    Detects human Gastrokine 1 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant mouse Gastrokine 1 is observed.
  • Source
    Monoclonal Mouse IgG1 Clone # 665701
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    E. coli-derived recombinant human Gastrokine 1
    Asn35-Asn199
    Accession # Q9NS71
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Immunohistochemistry
    8-25 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Immunohistochemistry
Gastrokine 1 in Human Stomach. Gastrokine 1 was detected in immersion fixed paraffin-embedded sections of human stomach using Mouse Anti‑Human Gastrokine 1 Monoclonal Antibody (Catalog # MAB6395) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to gastric glands. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Preparation and Storage
  • Reconstitution
    Sterile PBS to a final concentration of 0.5 mg/mL.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Gastrokine 1
Gastrokine 1 (GKN1; also CA11 and AMP-18) is an 18 kDa member of the CA11 protein family. It has limited expression, being restricted to mucous secreting pyloric atrial epithelium. Gastrokine 1 appears to promote epithelial proliferation and migration, and induce the formation of tight junctions between epithelial cells. Mature human Gastrokine 1 is 165 amino acids (aa) in length. Based on the SwissProt sequence, it possesses one BRICHOS domain (aa 54-150) that contains a mitogenic sequence (aa 97-117). There is one potential alternative start site 14 aa upstream of the standard site that does not appear to be utilized. Over aa 21-185 (aa 35-199 of SwissProt), human Gastrokine 1 shares 65% aa identity with mouse Gastrokine 1.  
  • Long Name:
    Take out Column D name
  • Entrez Gene IDs:
    56287 (Human); 66283 (Mouse); 297418 (Rat)
  • Alternate Names:
    18 kDa antrum mucosa protein; AMP18; AMP-18; AMP18BRICHOS domain containing 1; BRICD1; CA11; CA11MGC70354; FOV; Foveolin; Gastrokine 1; gastrokine-1; GKN1; Protein CA11
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Anti-Mouse HRP-DAB Cell & Tissue Staining Kit

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Mouse IgG1 Isotype Control

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Mouse/Rabbit IgG VisUCyte HRP Polymer Antibody

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Goat Anti-Mouse IgG Fc Affinity Purified Polyclonal Ab

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