Human GATA-4 Biotinylated Antibody Summary
Accession # P43694
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
GATA‑4 in iBJ6 iPS Cell Line. GATA-4 was detected in immersion fixed iBJ6, an induced pluripotent stem cell line, differentiated to endoderm using Goat Anti-Human GATA-4 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF2606) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Streptavidin (red; Catalog # NL999) and counterstained with DAPI (blue). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Stem Cells on Coverslips.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
GATA-4 belongs to the GATA family of transcription factors, which bind to the consensus DNA sequence (A/T) GATA (A/G) to control diverse tissue-specific programs of gene expression and morphogenesis. It is widely expressed in mesodermal- and endodermal-derived tissues. GATA-4 interacts with other transcription factors such as NKX2.5, and myocyte enhancer factor 2 to regulate cardiac myocyte-specific gene expression. Human GATA-4 share 93%, 93% and 92% amino acid sequence identity with porcine, rat and mouse GATA-4, respectively.
Citations for Human GATA-4 Biotinylated Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
Citations: Showing 1 - 2
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Development of Human Adrenocortical Adenoma (HAA1) Cell Line from Zona Reticularis
Authors: HK Ghayee, Y Xu, H Hatch, R Brockway, AS Multani, T Gu, WB Bollag, A Turcu, WE Rainey, J Rege, K Nanba, VJ Bhagwandin, F Nwariaku, V Stastny, AF Gazdar, JW Shay, RJ Auchus, SG Tevosian
International Journal of Molecular Sciences, 2022;24(1):.
Sample Types: Whole Cell
Isolation of primitive endoderm, mesoderm, vascular endothelial and trophoblast progenitors from human pluripotent stem cells.
Authors: Drukker M, Tang C, Ardehali R
Nat. Biotechnol., 2012;30(6):531-42.
Sample Types: Whole Cells
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