GATA‑4 in iBJ6 iPS Cell Line.
GATA‑4 was detected in immersion fixed iBJ6, an induced pluripotent stem cell line, differentiated to endoderm using Goat Anti-Human GATA‑4 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF2606) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Streptavidin (red; Catalog # NL999) and counterstained with DAPI (blue). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Stem Cells on Coverslips.
Preparation and Storage
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
GATA-4 belongs to the GATA family of transcription factors, which bind
to the consensus DNA sequence (A/T) GATA (A/G) to control diverse
tissue-specific programs of gene expression and morphogenesis. It is
widely expressed in mesodermal- and endodermal-derived tissues. GATA-4
interacts with other transcription factors such as NKX2.5, and myocyte
enhancer factor 2 to regulate cardiac myocyte-specific gene expression.
Human GATA-4 share 93%, 93% and 92% amino acid sequence identity with
porcine, rat and mouse GATA-4, respectively.
Entrez Gene IDs:
GATA binding protein 4; GATA4; GATA-4; GATA-binding factor 4; GATA-binding protein 4; MGC126629; transcription factor GATA-4
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