Human GATA‑4 Biotinylated Antibody

R&D Systems | Catalog # BAF2606

R&D Systems
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human, Transgenic Mouse

Applications

Validated:

Western Blot, Immunocytochemistry

Cited:

Immunohistochemistry, Immunocytochemistry

Label

Biotin

Antibody Source

Polyclonal Goat IgG
View all GATA-4 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human GATA-4
Met27-Phe211
Accession # P43694

Specificity

Detects human GATA‑4 in Western blots. In Western blots, less than 2% cross-reactivity with recombinant human GATA-1, -2, -3, -5, and -6 is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human GATA‑4 Biotinylated Antibody

GATA-4 antibody in iBJ6 iPS Cell Line by Immunocytochemistry (ICC).

GATA‑4 in iBJ6 iPS Cell Line.

GATA-4 was detected in immersion fixed iBJ6, an induced pluripotent stem cell line, differentiated to endoderm using Goat Anti-Human GATA-4 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF2606) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Streptavidin (red; Catalog # NL999) and counterstained with DAPI (blue). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Stem Cells on Coverslips.

Applications for Human GATA‑4 Biotinylated Antibody

Application
Recommended Usage

Immunocytochemistry

5-15 µg/mL
Sample: Immersion fixed endoderm differentiated BG01V human embryonic stem cells and iBJ6, an induced pluripotent stem (iPS) cell line, differentiated to endoderm

Western Blot

0.1 µg/mL
Sample: Recombinant Human GATA‑4

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: GATA-4

GATA-4 belongs to the GATA family of transcription factors, which bind to the consensus DNA sequence (A/T) GATA (A/G) to control diverse tissue-specific programs of gene expression and morphogenesis. It is widely expressed in mesodermal- and endodermal-derived tissues. GATA-4 interacts with other transcription factors such as NKX2.5, and myocyte enhancer factor 2 to regulate cardiac myocyte-specific gene expression. Human GATA-4 share 93%, 93% and 92% amino acid sequence identity with porcine, rat and mouse GATA-4, respectively.

Alternate Names

GATA4

Entrez Gene IDs

2626 (Human)

Gene Symbol

GATA4

UniProt

P43694

Additional GATA-4 Products

Product Documents for Human GATA‑4 Biotinylated Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human GATA‑4 Biotinylated Antibody

For research use only

Citations for Human GATA‑4 Biotinylated Antibody

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Protocols

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