Human GFR alpha -like Antibody

Recombinant Monoclonal Antibody
Catalog # Availability Size / Price Qty
MAB9697-100
MAB9697-SP
Human GFR alpha -like Antibody in Flow Cytometry
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Human GFR alpha -like Antibody Summary

Species Reactivity
Human
Specificity
Detects human GFR alpha ‑like in direct ELISAs.
Source
Recombinant Monoclonal Rabbit IgG Clone # 2345C
Purification
Protein A or G purified from cell culture supernatant
Immunogen
Human embryonic kidney cell line HEK293-derived recombinant human GFR alpha ‑like
Ser19-Glu351
Accession # Q6UXV0
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Flow Cytometry
0.25 µg/106 cells
See below
Blockade of Receptor-ligand Interaction
In a functional ELISA binding assay, 100-800 ng/mL of this antibody will block 50% of the binding of 10 ng/mL of Recombinant Human GDF-15 (Catalog # 9279-GD) to immobilized Recombinant Human GFR alpha -like Fc Chimera (Catalog # 9697-GR) coated at 0.1 μg/mL (100 μL/well). At 10 μg/mL, this antibody will block >90% of the binding.
 
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Flow Cytometry Detection of GFR alpha -like  in HEK293 Human Cell Line Transfected with Human GFR alpha -like by Flow  Cytometry. View Larger

Detection of GFR alpha -like in HEK293 Human Cell Line Transfected with Human GFR alpha -like by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with either (A) human GFR alpha -like (filled histogram) or (B) irrelevant transfectants (open histogram) was stained with Rabbit Anti-Human GFR alpha -like Monoclonal Antibody (Catalog # MAB9697) followed by Allophycocyanin-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # F0111). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: GFR alpha-like

GFR alpha -like (GDNF receptor-alpha-like) is a distant member of the GDNFR family of proteins (1). Mature human GFR alpha-like is a 376 amino acid (aa) type I transmembrane protein. It contains a 333 aa extracellular domain, a 20 aa transmembrane domain and a 23 aa cytoplasmic domain. Over the extracellular domain, human GFRAL shares 72% and 71%  identity with mouse and rat GFRAL respectively. It is expressed on both fetal and adult hindbrain neurons of the CNS (3), and would appear to function as an anti-apoptotic molecule during neuronal stress. GFRAL is a functional receptor for GDF-15, facilitating weight-loss functions of the protein through c-Ret downstream signaling (2-4). GFRAL and GDF-15 signaling is implicated in diet-based obesity and insulin resistance (2-4).

References
  1. Li, Z. et al. (2005) J. Neurochem. 95:361.
  2. Mullican, S. et al. (2017) Nat. Med 23:1150.
  3. Yang, L. et al. (2017) Nat. Med 23: 1158.
  4. Emmerson, P. et al (2017) Nat. Med 23:1215.
Long Name
Glial Cell line-derived Neurotrophic Factor Receptor alpha-like
Entrez Gene IDs
389400 (Human); 404194 (Mouse); 501023 (Rat)
Alternate Names
GFR alpha like; GFR alpha-like; GFRAL; GRAL

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