Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Western Blot, ELISA Detection (Matched Antibody Pair)

Cited:

ELISA Development, Luminex Development

Label

Biotin

Antibody Source

Monoclonal Mouse IgG1 Clone # 3209
View all GM-CSF Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human GM-CSF

Specificity

Detects recombinant human GM-CSF in Western blots.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Applications for Human GM‑CSF Biotinylated Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: Recombinant Human GM-CSF (Catalog # 215-GM)
under non-reducing conditions only

Human GM-CSF Sandwich Immunoassay

ELISA Detection (Matched Antibody Pair)
Recommended Concentration: 0.5-2.0 µg/mL
Use in combination with these reagents:
  • Capture Reagent: Human GM‑CSF Antibody (Catalog # MAB615)
  • Standard: Recombinant Human GM-CSF Protein (Catalog # 215-GM)
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 2 reviews rated 4.5 using BAM215 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from ascites

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: GM-CSF

Granulocyte macrophage colony stimulating factor (GM-CSF) is a growth factor produced by a variety of lymphoid cells. It binds to receptor heterodimers consisting of a GM-CSF-specific alpha chain and the common beta chain that is shared by the high-affinity receptors for IL-3 and IL-5.

Long Name

Granulocyte Macrophage Growth Factor

Alternate Names

CSF-2, CSF2, GMCSF, Molgramostim, Sargramostim

Entrez Gene IDs

1437 (Human); 12981 (Mouse); 116630 (Rat); 397208 (Porcine); 403923 (Canine); 493805 (Feline)

Gene Symbol

CSF2

Additional GM-CSF Products

Product Documents for Human GM‑CSF Biotinylated Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human GM‑CSF Biotinylated Antibody

For research use only

Citations for Human GM‑CSF Biotinylated Antibody

Customer Reviews for Human GM‑CSF Biotinylated Antibody (2)

4.5 out of 5
2 Customer Ratings
5 Stars
50%
4 Stars
50%
3 Stars
0%
2 Stars
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1 Stars
0%

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Customer Images

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  • Human GM-CSF Biotinylated Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: T cells
    Species: Human
    Verified Customer | Posted 12/10/2021
    Human GM‑CSF Biotinylated Antibody BAM215
  • Human GM-CSF Biotinylated Antibody
    Name: Anonymous
    Application: ELISA
    Sample Tested: Serum and Plasma
    Species: Human
    Verified Customer | Posted 11/02/2017
    This antibody was used as a matched pair with MAB615 to develop a sandwich ELISA. Human serum and plasma samples were successfully measured using the pair.
    Human GM‑CSF Biotinylated Antibody BAM215

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FAQs

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