Human GM-CSF Antibody

Catalog # Availability Size / Price Qty
MAB615-SP
MAB615-100
MAB615-500
Detection of GM‑CSF in Human Dendritic Cells by Flow Cytometry.
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Product Details
Citations (6)
FAQs
Supplemental Products
Reviews (2)

Human GM-CSF Antibody Summary

Species Reactivity
Human
Specificity
Detects human GM-CSF in ELISAs. In ELISAs, no cross-reactivity with recombinant mouse GM‑CSF, recombinant rat GM‑CSF, recombinant human (rh) M‑CSF, rhG‑CSF, rhIL‑5‑R alpha, or rhIL‑3 R alpha is observed.
Source
Monoclonal Mouse IgG1 Clone # 6804
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human GM-CSF
Ala18-Glu144
Accession # P04141
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS and NaCl with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 
Intracellular Staining by Flow Cytometry
0.25 µg/106 cells
Human mature dendritic cells treated with LPS, Recombinant Human TNF‑ alpha (Catalog # 210‑TA), and Recombinant Human IL‑1 beta /IL‑1F2 (Catalog # 201-LB) fixed with paraformaldehyde and permeabilized with saponin

Human GM-CSF Sandwich Immunoassay

Recommended Concentration
Reagent
ELISA Capture (Matched Antibody Pair)
2-8 µg/mL 

Use in combination with:

Detection Reagent: Human GM‑CSF Biotinylated Antibody (Catalog # BAM215)

Standard: Recombinant Human GM-CSF Protein (Catalog # 215-GM)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Intracellular Staining by Flow Cytometry View Larger

Detection of GM‑CSF in Human Dendritic Cells by Flow Cytometry. Human dendritic cells treated with LPS, Recombinant Human TNF-a (210-TA), and Recombinant Human TGF-beta 1 (240-B) were stained with Mouse Anti-Human GM-CSF Monoclonal Antibody (Catalog # MAB615, filled histogram) or isotype control antibody (IC002P, open histogram) followed by Goat anti-Mouse IgG PE-conjugated Secondary Antibody (F0102B). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (FC005). Staining was performed using our Staining Intracellular Molecules protocol.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: GM-CSF

GM-CSF is a growth factor produced by a variety of lymphoid cells. It binds to receptor heterodimers consisting of a GM-CSF-specific alpha chain and the common beta  chain that is shared by the high affinity receptors for IL-3 and IL-5.

Long Name
Granulocyte Macrophage Growth Factor
Entrez Gene IDs
1437 (Human); 12981 (Mouse); 116630 (Rat); 397208 (Porcine); 403923 (Canine); 493805 (Feline)
Alternate Names
colony stimulating factor 2 (granulocyte-macrophage); Colony-stimulating factor; CSF; CSF2; CSF-2; GMCSF; GM-CSF; GMCSFgranulocyte-macrophage colony-stimulating factor; granulocyte-macrophage colony stimulating factor; MGC131935; MGC138897; Molgramostim; molgramostin; Sargramostim

Product Datasheets

Citations for Human GM-CSF Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

6 Citations: Showing 1 - 6
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  1. A digital single-molecule nanopillar SERS platform for predicting and monitoring immune toxicities in immunotherapy
    Authors: J Li, A Wuethrich, AAI Sina, HH Cheng, Y Wang, A Behren, PN Mainwaring, M Trau
    Nature Communications, 2021;12(1):1087.
    Species: Human
    Sample Types: Serum
    Applications: ELISA Development
  2. Azithromycin decreases NALP3 mRNA stability in monocytes to limit inflammasome-dependent inflammation
    Authors: EA Lendermon, TA Coon, JS Bednash, NM Weathingto, JF McDyer, RK Mallampall
    Respir. Res., 2017;18(1):131.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  3. A multiplex immunoassay for human adipokine profiling.
    Authors: Schipper HS, De Jager W, van Dijk ME, Meerding J, Zelissen PM, Adan RA, Prakken BJ, Kalkhoven E
    Clin. Chem., 2010;56(0):1320.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: Luminex Development
  4. Upregulation of human cytomegalovirus by HIV type 1 in human lymphoid tissue ex vivo.
    Authors: Biancotto A, Iglehart SJ, Lisco A, Vanpouille C, Grivel JC, Lurain NS, Reichelderfer PS, Margolis LB
    AIDS Res. Hum. Retroviruses, 2008;24(3):453-62.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: Luminex Development
  5. Abnormal activation and cytokine spectra in lymph nodes of people chronically infected with HIV-1.
    Authors: Biancotto A, Grivel JC, Iglehart SJ, Vanpouille C, Lisco A, Sieg SF, Debernardo R, Garate K, Rodriguez B, Margolis LB, Lederman MM
    Blood, 2007;109(10):4272-9.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: Luminex Development
  6. Selective induction of eotaxin release by interleukin-13 or interleukin-4 in human airway smooth muscle cells is synergistic with interleukin-1beta and is mediated by the interleukin-4 receptor alpha-chain.
    Authors: Hirst SJ, Hallsworth MP, Peng Q
    Am. J. Respir. Crit. Care Med., 2002;165(8):1161-71.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: ELISA Development

FAQs

  1. Do you have a protocol for the isolation and treatment of dendritic cells used for IC flow staining with Human GM-CSF Antibody, catalog #s MAB615 and IC615?

    • MagCellect Human CD14+ Cell Isolation Kit, catalog # MAGH105, is used to isolate CD14+ monocytes from PBMCs, as the starting population.  Monocytes are cultured in media with rhIL-4 (catalog # 204-IL) at 20 ng/ml and rhGM-CSF (catalog # 215-GM) at 50ng/ml. On day 6, add LPS at 20ng/ml, rhTNF-a (catalog # 210-TA) at 20ng/ml and rhIL-1b (catalog # 201-LB) at 20ng/ml to culture medium and incubate cells overnight. Collect cells for flow cytometric analysis on day 7.

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Isotype Controls

Reconstitution Buffers

Secondary Antibodies

Reviews for Human GM-CSF Antibody

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Human GM-CSF Antibody
By Anonymous on 12/13/2017
Application: Affinity Purification Sample Tested: CHO Chinese hamster ovary cell line Species: Hamster

Human GM-CSF Antibody
By Anonymous on 11/02/2017
Application: ELISA Sample Tested: Serum and Plasma Species: Human

This antibody was used as a capture in developing a sandwich ELISA for measuring GM-CSF in human serum and plasma. BAM215 paired very well with this antibody.