Human GM130/GOLGA2 Antibody Summary
Accession # Q08379
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of Human GM130/GOLGA2 by Western Blot. Western blot shows lysates of MCF‑7 human breast cancer cell line and ZR‑75 human breast cancer cell line. PVDF membrane was probed with 0.25 µg/mL of Sheep Anti-Human GM130/GOLGA2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8199) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for GM130/GOLGA2 at approximately 130 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
GM130/GOLGA2 in HeLa Human Cell Line. GM130/GOLGA2 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Sheep Anti-Human GM130/GOLGA2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8199) at 1.7 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). Specific staining was localized to Golgi apparatus vesicles. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
GM130, also known as Golgin subfamily A member 2 and GOLGA2, is a 130 kDa member of the Golgin protein family. GM130/GOLGA2 is a component of the Golgi membrane involved in maintaining cis-Golgi structure. GM130/GOLGA2 protein is thought to play roles in the stacking of Golgi cisternae and in vesicular transport. Several alternatively spliced transcript variants of this gene have been predicted. Human GM130/GOLGA2 protein is 1002 amino acids (aa) in length, and over aa 528‑606, shares 90% sequence identity with its mouse and rat orthologs.
Citations for Human GM130/GOLGA2 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
Citations: Showing 1 - 3
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The potassium channel KCNJ13 is essential for smooth muscle cytoskeletal organization during mouse tracheal tubulogenesis
Authors: W Yin, HT Kim, S Wang, F Gunawan, L Wang, K Kishimoto, H Zhong, D Roman, J Preussner, S Guenther, V Graef, C Buettner, B Grohmann, M Looso, M Morimoto, G Mardon, S Offermanns, DYR Stainier
Nat Commun, 2018;9(1):2815.
Sample Types: Whole Tissue
Endosomal phosphatidylserine is critical for the YAP signalling pathway in proliferating cells
Authors: T Matsudaira, K Mukai, T Noguchi, J Hasegawa, T Hatta, SI Iemura, T Natsume, N Miyamura, H Nishina, J Nakayama, K Semba, T Tomita, S Murata, H Arai, T Taguchi
Nat Commun, 2017;8(1):1246.
Sample Types: Whole Cells
CLIPR-59: a protein essential for neuromuscular junction stability during mouse late embryonic development.
Authors: Couesnon A, Offner N, Bernard V, Chaverot N, Backer S, Dimitrov A, Perez F, Molgo J, Bloch-Gallego E
Sample Types: Whole Cells
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