Human GM130/GOLGA2 Antibody

Catalog # Availability Size / Price Qty
Human GM130/GOLGA2 Antibody in Western Blot
2 Images
Product Details
Citations (3)
Supplemental Products

Human GM130/GOLGA2 Antibody Summary

Species Reactivity
Detects human GM130/GOLGA2 in direct ELISAs and Western blots.   
Polyclonal Sheep IgG
Antigen Affinity-purified
E. coli-derived recombinant human GM130/GOLGA2
Accession # Q08379
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.


Recommended Concentration
Western Blot
0.25 µg/mL
See below
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Western Blot Detection of Human GM130/GOLGA2 by Western Blot. View Larger

Detection of Human GM130/GOLGA2 by Western Blot. Western blot shows lysates of MCF‑7 human breast cancer cell line and ZR‑75 human breast cancer cell line. PVDF membrane was probed with 0.25 µg/mL of Sheep Anti-Human GM130/GOLGA2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8199) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for GM130/GOLGA2 at approximately 130 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunocytochemistry GM130/GOLGA2 in HeLa Human Cell Line. View Larger

GM130/GOLGA2 in HeLa Human Cell Line. GM130/GOLGA2 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Sheep Anti-Human GM130/GOLGA2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8199) at 1.7 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). Specific staining was localized to Golgi apparatus vesicles. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Preparation and Storage

Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: GM130/GOLGA2

GM130, also known as Golgin subfamily A member 2 and GOLGA2, is a 130 kDa member of the Golgin protein family.  GM130/GOLGA2 is a component of the Golgi membrane involved in maintaining cis-Golgi structure.  GM130/GOLGA2 protein is thought to play roles in the stacking of Golgi cisternae and in vesicular transport. Several alternatively spliced transcript variants of this gene have been predicted. Human GM130/GOLGA2 protein is 1002 amino acids (aa) in length, and over aa 528‑606, shares 90% sequence identity with its mouse and rat orthologs.

Long Name
Golgin A2
Entrez Gene IDs
2801 (Human); 99412 (Mouse)
Alternate Names
130 kDa cis-Golgi matrix protein; GM130 autoantigen; GM130; GM130Golgi matrix protein GM130; GOLGA2; golgi autoantigen, golgin subfamily a, 2; golgin A2; Golgin subfamily A member 2; Golgin-95; MGC20672; SY11 Protein

Product Datasheets

Citations for Human GM130/GOLGA2 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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  1. The potassium channel KCNJ13 is essential for smooth muscle cytoskeletal organization during mouse tracheal tubulogenesis
    Authors: W Yin, HT Kim, S Wang, F Gunawan, L Wang, K Kishimoto, H Zhong, D Roman, J Preussner, S Guenther, V Graef, C Buettner, B Grohmann, M Looso, M Morimoto, G Mardon, S Offermanns, DYR Stainier
    Nat Commun, 2018;9(1):2815.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  2. Endosomal phosphatidylserine is critical for the YAP signalling pathway in proliferating cells
    Authors: T Matsudaira, K Mukai, T Noguchi, J Hasegawa, T Hatta, SI Iemura, T Natsume, N Miyamura, H Nishina, J Nakayama, K Semba, T Tomita, S Murata, H Arai, T Taguchi
    Nat Commun, 2017;8(1):1246.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  3. CLIPR-59: a protein essential for neuromuscular junction stability during mouse late embryonic development.
    Authors: Couesnon A, Offner N, Bernard V, Chaverot N, Backer S, Dimitrov A, Perez F, Molgo J, Bloch-Gallego E
    Development, 2013;140(7):1583-93.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: IHC


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