Key Product Details

Species Reactivity

Human

Applications

Flow Cytometry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 1055815
View all GPR116 Primary Antibodies »

Product Specifications

Immunogen

E. coli-expressed recombinant Human GPR116 extracellular domain.
His643-Asn945
Accession # Q8IZF2

Specificity

Detects Human GPR116 in Direct ELISAs. In Flow Cytometry, it detects human GPR116 in transfected cells, but not in non-transfected parental cell line.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human GPR116 Antibody

Detection of GPR116 in HEK293 human cell line transfected with human GPR116 by Flow Cytometry.

HEK293 human cell line transfected with human GPR116 was stained with either (A) Mouse Anti-Human GPR116 Monoclonal Antibody (Catalog # MAB112481) or (B) Mouse IgG1 Isotype Control (MAB002), followed by APC-conjugated anti-mouse IgG secondary antibody (F0101B). Staining was performed using our Staining Membrane-associated Proteins.

Applications for Human GPR116 Antibody

Application
Recommended Usage

Flow Cytometry

25 µg/mL
Sample: HEK293 human cell line transfected with human GPR116

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: GPR116

GPR116, also known as  Adhesion G protein-coupled receptor F5 or ADGRF5, belongs to the LN-TM7 subfamily of the G protein-coupled receptor 2 family, also known as adhesion GPCRs. It exists as a highly glycosylated disulfide-linked dimer at the cell surface. GPR116 may have a role in the regulation of acid-base balance and is also being investigated for it's involvement in adipocyte biology.

Long Name

G Protein-coupled Receptor 116

Alternate Names

FLJ90640, G protein-coupled receptor 116, Ig-Hepta homolog, KIAA0758DKFZp564O1923, KPG_001, probable G-protein coupled receptor 116

Entrez Gene IDs

221395 (Human); 224792 (Mouse); 245977 (Rat)

Gene Symbol

ADGRF5

UniProt

Q8IZF2

Additional GPR116 Products

Product Documents for Human GPR116 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human GPR116 Antibody

For research use only

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