Human GPRC5C Antibody

R&D Systems | Catalog # MAB6594

R&D Systems

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Flow Cytometry, CyTOF-ready

Cited:

Proximity Ligation Assay

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # 577315
View all GPRC5C Primary Antibodies »

Product Specifications

Immunogen

NS0 mouse myeloma cell line transfected with recombinant human GPRC5C
Accession # Q9NQ84

Specificity

Detects human GPRC5C in direct ELISAs.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for Human GPRC5C Antibody

Detection of GPRC5C antibody in PC-3 Human Cell Line antibody by Flow Cytometry.

Detection of GPRC5C in PC-3 Human Cell Line by Flow Cytometry.

PC-3 human prostate cancer cell line was stained with Human GPRC5C Monoclonal Antibody (Catalog # MAB6594, filled histogram) or isotype control antibody (Catalog # MAB003, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG F(ab')2Secondary Antibody (Catalog # F0102B).

Applications for Human GPRC5C Antibody

Application
Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

0.25 µg/106 cells
Sample: PC‑3 human prostate cancer cell line

Flow Cytometry Panel Builder

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Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: GPRC5C

G-protein coupled receptor family C group 5 member C (GPRC5C), also known as Retinoic acid induced gene 3 (RAIG-3), is an approximately 50 kDa protein with seven transmembrane segments and one site of potential N-linked glycosylation. It is predominantly expressed in the stomach, kidney, liver, pancreas, and prostate. Human GPRC5C shares 88% and 90% amino acid sequence identity with mouse and rat GPRC5C, respectively.

Long Name

G Protein-coupled Receptor, Family C, Group 5, Member C

Alternate Names

RAIG3

Entrez Gene IDs

55890 (Human)

Gene Symbol

GPRC5C

UniProt

Q9NQ84

Additional GPRC5C Products

Product Documents for Human GPRC5C Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human GPRC5C Antibody

For research use only

Citations for Human GPRC5C Antibody

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