Human GPRC5C Antibody

Catalog #: MAB6594 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB6594
MAB6594-SP

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Detection of GPRC5C in PC-3 Human Cell Line by Flow Cytometry.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human GPRC5C Antibody Summary

Species Reactivity

Human

Specificity

Detects human GPRC5C in direct ELISAs.

Source

Monoclonal Mouse IgG_2A Clone # 577315

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

NS0 mouse myeloma cell line transfected with recombinant human GPRC5C
Accession # Q9NQ84

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Flow Cytometry

0.25 µg/10⁶ cells

See below

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of GPRC5C in PC-3 Human Cell Line by Flow Cytometry. PC-3 human prostate cancer cell line was stained with Human GPRC5C Monoclonal Antibody (Catalog # MAB6594, filled histogram) or isotype control antibody (Catalog # MAB003, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG F(ab')₂Secondary Antibody (Catalog # F0102B).

Reconstitution Calculator

Preparation and Storage

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL.

Reconstitution Buffer Available

Reconstitution Buffer 1 (PBS)

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: GPRC5C

G-protein coupled receptor family C group 5 member C (GPRC5C), also known as Retinoic acid induced gene 3 (RAIG-3), is an approximately 50 kDa protein with seven transmembrane segments and one site of potential N-linked glycosylation. It is predominantly expressed in the stomach, kidney, liver, pancreas, and prostate. Human GPRC5C shares 88% and 90% amino acid sequence identity with mouse and rat GPRC5C, respectively.

Long Name

G Protein-coupled Receptor, Family C, Group 5, Member C

Entrez Gene IDs

55890 (Human)

Alternate Names

G protein-coupled receptor, family C, group 5, member C; GPRC5C; MGC131820; RAIG3; RAIG-3G-protein coupled receptor family C group 5 member C; RAIG3orphan G-protein coupled receptor; retinoic acid responsive gene protein; Retinoic acid-induced gene 3 protein