Human Granzyme H Antibody [Biotin]

R&D Systems | Catalog # BAF1377

R&D Systems

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Western Blot, Immunocytochemistry

Cited:

Flow Cytometry

Label

Biotin

Antibody Source

Polyclonal Goat IgG
View all Granzyme H Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Granzyme H
Glu19-Leu246
Accession # P20718

Specificity

Detects both the pro and active forms of human Granzyme H in direct ELISAs and Western blots. In direct ELISAs, approximately 25% cross‑reactivity with recombinant human Granzyme B is observed and approximately 5% cross-reactivity with recombinant mouse (rm) Granzyme C, rmGranzyme D, and rmGranzyme G is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human Granzyme H Antibody [Biotin]

Granzyme H antibody in Human PBMCs by Immunocytochemistry (ICC).

Granzyme H in Human PBMCs.

Granzyme H was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) using Goat Anti-Human Granzyme H Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF1377) at 5 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Streptavidin (red; Catalog # NL999) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Applications for Human Granzyme H Antibody [Biotin]

Application
Recommended Usage

Immunocytochemistry

5-15 µg/mL
Sample: Immersion fixed human peripheral blood mononuclear cells

Western Blot

0.1 µg/mL
Sample: Recombinant Human Granzyme H (Catalog # 1377-SE)

Reviewed Applications

Read 1 review rated 3 using BAF1377 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Granzyme H

Granzyme H is a member of the granzyme family of serine proteases found specifically in the cytotoxic granules of cytotoxic T lymphocytes (CTL) and natural killer (NK) cells (1, 2). Granzyme H’s functions are largely unknown. The more abundant expression of Granzyme H than Granzyme B in NK cells suggests that Granzyme H may complement the pro-apoptotic function of Granzyme B in this cell type (3). Human Granzyme H shows the highest amino acid identity (71%) to mouse Granzyme C (4). Human Granzyme H is synthesized as a precursor (246 residues) with a signal peptide (residues 1‑18), a propeptide (residues 19‑20) and a mature chain (residues 21‑246) (5‑7). The pro-enzyme is expressed and purified. After being activated by active cathepsin C, rhGranzyme H cleaves a thioester substrate, which was described previously (8).

References

  1. Kam, C.-M. et al. (2000) Biochim. Biophys. Acta 1477:307.
  2. Smyth, M.J. et al. (1996) J. Leukoc. Biol. 60:555.
  3. Sedelies, K.A. et al. (2004) J. Biol. Chem. 279:26581.
  4. Sattar, R. et al. (2003) Biochem. Biophys. Res. Comm. 308:726.
  5. Meier, M. et al. (1990) Biochemistry 29:4042.
  6. Haddad, P. et al. (1991) Int. Immunol. 3:57.
  7. Klein, J.L. et al. (1990) Tissue Antigens 35:220.
  8. Edwards, K.M. et al. (1999) J. Biol. Chem. 274:30468.

Alternate Names

CCP-X, CGL2, CTSGL2, GrzH, GZMH

Entrez Gene IDs

2999 (Human)

Gene Symbol

GZMH

UniProt

P20718

Additional Granzyme H Products

Product Documents for Human Granzyme H Antibody [Biotin]

Certificate of Analysis

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Product Specific Notices for Human Granzyme H Antibody [Biotin]

For research use only

Citations for Human Granzyme H Antibody [Biotin]

Customer Reviews for Human Granzyme H Antibody [Biotin] (1)

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  • Human Granzyme H Biotinylated Antibody
    Name: Anonymous
    Application: Flow Cytometry
    Sample Tested: Cell Culture Samples
    Species: Human
    Verified Customer | Posted 08/14/2022
    Human Granzyme H Antibody [Biotin] BAF1377

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