Human Granzyme H Biotinylated Antibody

Catalog # Availability Size / Price Qty
Granzyme H  in Human PBMCs.
1 Image
Product Details
Supplemental Products

Human Granzyme H Biotinylated Antibody Summary

Species Reactivity
Detects both the pro and active forms of human Granzyme H in direct ELISAs and Western blots. In direct ELISAs, approximately 25% cross‑reactivity with recombinant human Granzyme B is observed and approximately 5% cross-reactivity with recombinant mouse (rm) Granzyme C, rmGranzyme D, and rmGranzyme G is observed.
Polyclonal Goat IgG
Antigen Affinity-purified
Mouse myeloma cell line NS0-derived recombinant human Granzyme H
Accession # P20718
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.


Recommended Concentration
Western Blot
0.1 µg/mL
Recombinant Human Granzyme H (Catalog # 1377-SE)
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Immunocytochemistry Granzyme H  in Human PBMCs. View Larger

Granzyme H in Human PBMCs. Granzyme H was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) using Goat Anti-Human Granzyme H Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF1377) at 5 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Streptavidin (red; Catalog # NL999) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Preparation and Storage

Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Granzyme H

Granzyme H is a member of the granzyme family of serine proteases found specifically in the cytotoxic granules of cytotoxic T lymphocytes (CTL) and natural killer (NK) cells (1, 2). Granzyme H’s functions are largely unknown. The more abundant expression of Granzyme H than Granzyme B in NK cells suggests that Granzyme H may complement the pro-apoptotic function of Granzyme B in this cell type (3). Human Granzyme H shows the highest amino acid identity (71%) to mouse Granzyme C (4). Human Granzyme H is synthesized as a precursor (246 residues) with a signal peptide (residues 1‑18), a propeptide (residues 19‑20) and a mature chain (residues 21‑246) (5‑7). The pro-enzyme is expressed and purified. After being activated by active cathepsin C, rhGranzyme H cleaves a thioester substrate, which was described previously (8).

  1. Kam, C.-M. et al. (2000) Biochim. Biophys. Acta 1477:307.
  2. Smyth, M.J. et al. (1996) J. Leukoc. Biol. 60:555.
  3. Sedelies, K.A. et al. (2004) J. Biol. Chem. 279:26581.
  4. Sattar, R. et al. (2003) Biochem. Biophys. Res. Comm. 308:726.
  5. Meier, M. et al. (1990) Biochemistry 29:4042.
  6. Haddad, P. et al. (1991) Int. Immunol. 3:57.
  7. Klein, J.L. et al. (1990) Tissue Antigens 35:220.
  8. Edwards, K.M. et al. (1999) J. Biol. Chem. 274:30468.
Entrez Gene IDs
2999 (Human)
Alternate Names
Cathepsin G-like 2; CCP-X; CCP-Xcytotoxic T-lymphocyte-associated serine esterase 1; CGL2; CGL-2; CSP-Ccytotoxin serine protease-C; CTLA1; CTSGL2; CTSGL2granzyme H; Cytotoxic serine protease C; Cytotoxic T-lymphocyte proteinase; EC 3.4.21; EC 3.4.21.-; EC; granzyme H (cathepsin G-like 2, protein h-CCPX); Granzyme H; GrzH; GZMH

Product Datasheets


No product specific FAQs exist for this product, however you may

View all Antibody FAQs

Isotype Controls

Reconstitution Buffers

Staining Reagents


Supplemental Cell Selection Products

Supplemental ELISA Products

Reviews for Human Granzyme H Biotinylated Antibody

There are currently no reviews for this product. Be the first to review Human Granzyme H Biotinylated Antibody and earn rewards!

Have you used Human Granzyme H Biotinylated Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review