Human Granzyme H Antibody

AF1377 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF1377
AF1377-SP

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Human Granzyme H Antibody Summary

Species Reactivity

Human

Specificity

Detects both the pro and active forms of human Granzyme H in direct ELISAs and Western blots. In direct ELISAs, approximately 25% cross‑reactivity with recombinant human Granzyme B is observed and approximately 5% cross-reactivity with recombinant mouse (rm) Granzyme C, rmGranzyme D, and rmGranzyme G is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Granzyme H
Glu19-Leu246
Accession # P20718

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

Endotoxin Level

<0.01 EU per 1 μg of the antibody by the LAL method.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Human Granzyme H (Catalog # 1377-SE)

Immunoprecipitation

25 µg/mL

Conditioned cell culture medium spiked with Recombinant Human Granzyme H (Catalog # 1377-SE), see our available Western blot detection antibodies

Immunocytochemistry

5-15 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Immunocytochemistry

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Granzyme H in Human PBMCs. Granzyme H was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) using Goat Anti-Human Granzyme H Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1377) at 5 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Reconstitution Buffer Available

Reconstitution Buffer 1 (PBS)

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Granzyme H

Granzyme H is a member of the granzyme family of serine proteases found specifically in the cytotoxic granules of cytotoxic T lymphocytes (CTL) and natural killer (NK) cells (1, 2). Granzyme H’s functions are largely unknown. The more abundant expression of Granzyme H than Granzyme B in NK cells suggests that Granzyme H may complement the pro-apoptotic function of Granzyme B in this cell type (3). Human Granzyme H shows the highest amino acid identity (71%) to mouse Granzyme C (4). Human Granzyme H is synthesized as a precursor (246 residues) with a signal peptide (residues 1‑18), a propeptide (residues 19‑20) and a mature chain (residues 21‑246) (5‑7). The pro-enzyme is expressed and purified. After being activated by active cathepsin C, rhGranzyme H cleaves a thioester substrate, which was described previously (8).

References

Kam, C.-M. et al. (2000) Biochim. Biophys. Acta 1477:307.
Smyth, M.J. et al. (1996) J. Leukoc. Biol. 60:555.
Sedelies, K.A. et al. (2004) J. Biol. Chem. 279:26581.
Sattar, R. et al. (2003) Biochem. Biophys. Res. Comm. 308:726.
Meier, M. et al. (1990) Biochemistry 29:4042.
Haddad, P. et al. (1991) Int. Immunol. 3:57.
Klein, J.L. et al. (1990) Tissue Antigens 35:220.
Edwards, K.M. et al. (1999) J. Biol. Chem. 274:30468.

Entrez Gene IDs

2999 (Human)

Alternate Names

Cathepsin G-like 2; CCP-X; CCP-Xcytotoxic T-lymphocyte-associated serine esterase 1; CGL2; CGL-2; CSP-Ccytotoxin serine protease-C; CTLA1; CTSGL2; CTSGL2granzyme H; Cytotoxic serine protease C; Cytotoxic T-lymphocyte proteinase; EC 3.4.21; EC 3.4.21.-; EC 3.4.21.79; granzyme H (cathepsin G-like 2, protein h-CCPX); Granzyme H; GrzH; GZMH