Human HCN4 Antibody

Catalog # Availability Size / Price Qty
AF8138
AF8138-SP
HCN4 in BG01V Human Embyonic Stem Cells.
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Human HCN4 Antibody Summary

Species Reactivity
Human
Specificity
Detects human HCN4 in direct ELISAs.
Source
Polyclonal Sheep IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human HCN4
Lys1084-Leu1203
Accession # Q9Y3Q4
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Immunocytochemistry
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Immunocytochemistry HCN4 in BG01V Human Embyonic Stem Cells. View Larger

HCN4 in BG01V Human Embyonic Stem Cells. HCN4 was detected in immersion fixed BG01V human embryonic stem cells, undifferentiated (lower panel) and differentiated into cardiomyocytes (upper panel), using Sheep Anti-Human HCN4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8138) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces. View our protocol for Fluorescent ICC Staining of Stem Cells on Coverslips.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: HCN4

HCN4 (hyperpolarization-activated cyclic nucleotide-gated channel 4) is a 120-160 kDa, 1203 amino acid (aa) multipass plasma membrane protein that is a member of the HCN family of potassium channel proteins. It is mainly expressed in cardiac myocytes, thalamus and testis. HCN4 is important for pacemaker function in the heart, and rhythmic activity in the thalamus. Specific mutations can cause Sick Sinus Syndrome 2 (SSS2), a cardiac sinus node malfunction, or Brugada syndrome 8 (BRGDA8), a tachyarrhythmia syndrome. Within aa 1084-1203, human HCN4 shares 92% and 93% aa sequence identity with mouse and rat HCN4, respectively.

Long Name
Hyperpolarization Activated Cyclic Nucleotide-Gated Potassium Channel 4
Entrez Gene IDs
10021 (Human); 330953 (Mouse); 59266 (Rat)
Alternate Names
HCN4; hyperpolarization activated cyclic nucleotide-gated cation channel 4; hyperpolarization activated cyclic nucleotide-gated potassium channel 4; potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 4; SSS2

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Human HCN4 Antibody
By Anonymous on 06/28/2016
Application: ICC/IF Sample Tested: cardiomyocyte Species: Human