Key Product Details

Species Reactivity

Human

Applications

Immunocytochemistry

Label

Unconjugated

Antibody Source

Polyclonal Sheep IgG
View all HCN4 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human HCN4
Lys1084-Leu1203
Accession # Q9Y3Q4

Specificity

Detects human HCN4 in direct ELISAs.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Scientific Data Images for Human HCN4 Antibody

HCN4 antibody in BG01V Human Embyonic Stem Cells by Immunocytochemistry (ICC).

HCN4 in BG01V Human Embyonic Stem Cells.

HCN4 was detected in immersion fixed BG01V human embryonic stem cells, undifferentiated (lower panel) and differentiated into cardiomyocytes (upper panel), using Sheep Anti-Human HCN4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8138) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces. View our protocol for Fluorescent ICC Staining of Stem Cells on Coverslips.

Applications for Human HCN4 Antibody

Application
Recommended Usage

Immunocytochemistry

5-15 µg/mL
Sample: Immersion fixed BG01V human embryonic stem cells differentiated into cardiomyocytes

Reviewed Applications

Read 1 review rated 5 using AF8138 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: HCN4

HCN4 (hyperpolarization-activated cyclic nucleotide-gated channel 4) is a 120-160 kDa, 1203 amino acid (aa) multipass plasma membrane protein that is a member of the HCN family of potassium channel proteins. It is mainly expressed in cardiac myocytes, thalamus and testis. HCN4 is important for pacemaker function in the heart, and rhythmic activity in the thalamus. Specific mutations can cause Sick Sinus Syndrome 2 (SSS2), a cardiac sinus node malfunction, or Brugada syndrome 8 (BRGDA8), a tachyarrhythmia syndrome. Within aa 1084-1203, human HCN4 shares 92% and 93% aa sequence identity with mouse and rat HCN4, respectively.

Long Name

Hyperpolarization Activated Cyclic Nucleotide-Gated Potassium Channel 4

Alternate Names

SSS2

Entrez Gene IDs

10021 (Human); 330953 (Mouse); 59266 (Rat)

Gene Symbol

HCN4

UniProt

Q9Y3Q4

Additional HCN4 Products

Product Documents for Human HCN4 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human HCN4 Antibody

For research use only

Customer Reviews for Human HCN4 Antibody (1)

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  • Human HCN4 Antibody
    Name: bo lin
    Application: Immunocytochemistry/Immunofluorescence
    Sample Tested: cardiomyocyte
    Species: Human
    Verified Customer | Posted 06/28/2016
    Human HCN4 Antibody AF8138

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