Human HIF-1 alpha Antibody

Recombinant Monoclonal Antibody
Catalog # Availability Size / Price Qty
MAB19351-100
MAB19351-SP
Detection  of Human HIF‑1 alpha  by Western Blot.
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Human HIF-1 alpha Antibody Summary

Species Reactivity
Human
Specificity
Detects human HIF-1 alpha in direct ELISAs.
Source
Recombinant Monoclonal Rabbit IgG1 Clone # 2443B
Purification
Protein A or G purified from cell culture supernatant
Immunogen
E. coli-derived recombinant human HIF-1 alpha
Arg575-Asn826
Accession # Q16665.1
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
2 µg/mL
See below
Immunocytochemistry
3-25 µg/mL
See below
Knockout Validated
HIF‑1 alpha is specifically detected in HeLa human cervical epithelial carcinoma parental cell line but is not detectable in HIF‑1 alpha knockout HeLa cell line.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Western Blot Detection  of Human HIF‑1 alpha  by Western Blot. View Larger

Detection of Human HIF‑1 alpha by Western Blot. Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line untreated (-) or treated (+) with 1 mM DFO for overnight. PVDF membrane was probed with 2 µg/mL of Rabbit Anti-Human HIF‑1 alpha Monoclonal Antibody (Catalog # MAB19351) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for HIF‑1 alpha at approximately 120 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunocytochemistry HIF‑1 alpha   in HeLa Human Cell Line. View Larger

HIF‑1 alpha in HeLa Human Cell Line. HIF‑1 alpha was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line treated with DFO (left panel) or untreated (right panel) using Rabbit Anti-Human HIF‑1 alpha Monoclonal Antibody (Catalog # MAB19351) at 3 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; Catalog # NL004) and counterstained with DAPI (blue). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Knockout Validated Western Blot Shows Human HIF‑1 alpha  Specificity by Using Knockout Cell Line. View Larger

Western Blot Shows Human HIF‑1 alpha Specificity by Using Knockout Cell Line. Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and HIF‑1 alpha knockout HeLa cell line (KO) untreated (-) or treated (+) with 1 mM DFO for overnight. PVDF membrane was probed with 2 µg/mL of Rabbit Anti-Human HIF‑1 alpha Monoclonal Antibody (Catalog # MAB19351) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for HIF‑1 alpha at approximately 120 kDa (as indicated) in the parental HeLa cell line, but is not detectable in knockout HeLa cell line. GAPDH (Catalog # MAB5718) is shown as a loading control.This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: HIF-1 alpha

The hypoxia-inducible transcription factor 1 alpha (HIF-1 alpha ) is the regulated member of the transcription factor heterodimer HIF-1. HIF-1 binds to hypoxia-response elements (HREs) in the promoters of many genes involved in adapting to an environment of insufficient oxygen or hypoxia. Hypoxic tissue environments occur in vascular and pulmonary diseases as well as cancer, which illustrates the broad impact of gene regulation by HIF-1 alpha.

Long Name
Hypoxia-inducible Transcription Factor 1 alpha
Entrez Gene IDs
3091 (Human); 15251 (Mouse); 29560 (Rat)
Alternate Names
ARNT-interacting protein; Basic-helix-loop-helix-PAS protein MOP1; BHLHE78; Class E basic helix-loop-helix protein 78; HIF 1A; HIF1 alpha; HIF-1 alpha; HIF1A; HIF-1a; HIF-1-alpha; HIF1-alpha; hypoxia inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor); hypoxia-inducible factor 1-alpha; Member of PAS protein 1; member of PAS superfamily 1; MOP1HIF1-ALPHA; PAS domain-containing protein 8; PASD8alpha subunit (basic helix-loop-helix transcription factor)

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