Human HLA-C Antibody

Catalog # Availability Size / Price Qty
MAB10394-100
MAB10394-SP
Human HLA-C Antibody in Flow Cytometry
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Human HLA-C Antibody Summary

Species Reactivity
Human
Specificity
Detects human HLA-C in direct ELISAs.
Source
Monoclonal Mouse IgG Clone # DT-9
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Purified tamarin MHC class I molecules (using anti-class I antibody BB7.7)
Accession # P10321
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Flow Cytometry
0.25 µg/106 cells
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Flow Cytometry Detection of HLA-C in Human PBMC Lymphocytes by Flow Cytometry. View Larger

Detection of HLA-C in Human PBMC Lymphocytes by Flow Cytometry. Human PBMC lymphocytes were stained with Mouse Anti-Human HLA-C Monoclonal Antibody (Catalog # MAB10394, filled histogram) or isotype control antibody (Catalog # MAB0041, open histogram), followed by PE-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: HLA-C

HLA-A, B, and C are approximately 45 kDa transmembrane glycoproteins in the major histocompatibility complex 1 (MHC I) family. They contain three alpha domains in their extracellular regions. HLA molecules are expressed on nearly all nucleated cells in association with the 12 kDa beta 2-Microglobulin. This complex binds peptides derived from pathogenic cytosolic or extracellular proteins such as viral or microbial proteins. It presents these peptides on the cell surface for recognition by the T cell receptor on CD8+ cytotoxic T cells. The activated cytotoxic T cell then kills the presenting cell. Mismatched MHC I alleles between a host and a donor lead to transplant rejection.

 

Long Name
Major Histocompatibility Complex, Class I, C
Entrez Gene IDs
3107 (Human)
Alternate Names
C alpha chain; D6S204; FLJ27082; HLA class I histocompatibility antigen, Cw-1 alpha chain; HLAC; HLA-C; HLA-Cw; HLA-JY3; HLC-C; human leukocyte antigen-C alpha chain; leucocyte antigen C; leucocyte antigen Cw; leukocyte antigen-C alpha chain; major histocompatibility antigen HLA-C; major histocompatibility complex, class I, C; MHC class I antigen Cw*1; MHC class I antigen Cw*12; MHC class I antigen Cw*14; MHC class I antigen Cw*15; MHC class I antigen Cw*16; MHC class I antigen Cw*17; MHC class I antigen Cw*2; MHC class I antigen Cw*3; MHC class I antigen Cw*4; MHC class I antigen Cw*5; MHC class I antigen Cw*6; MHC class I antigen Cw*7; MHC class I antigen Cw*8; MHC class I antigen heavy chain HLA-C; PSORS1

Product Datasheets

FAQs

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Reconstitution Buffers

Secondary Antibodies

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