Human HLA-DR Alexa Fluor® 488-conjugated Antibody

Catalog # Availability Size / Price Qty
FAB4869G-100UG
Detection of HLA-DR in Human Blood Lymphocytes by Flow Cytometry.
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Human HLA-DR Alexa Fluor® 488-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human HLA‑DR.
Source
Monoclonal Mouse IgG1 Clone # L203
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
RPMI 8866 human lymphoblastoid cells
Accession # P01903
Formulation
Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.
Label
Alexa Fluor 488 (Excitation= 488 nm, Emission= 515-545 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
0.25-1 µg/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Flow Cytometry Detection of HLA-DR in Human Blood Lymphocytes by Flow Cytometry. View Larger

Detection of HLA-DR in Human Blood Lymphocytes by Flow Cytometry. Human peripheral blood lymphocytes were stained with (A) Mouse Anti-Human HLA-DR Alexa Fluor® 488-conjugated Monoclonal Antibody (Catalog # FAB4869G) or (B) isotype control antibody (Catalog # IC002G) Mouse anti-Human CD19 APC-conjugated Monoclonal Antibody (Catalog # FAB4867A). View our protocol for Staining Membrane-associated Proteins.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: HLA-DR

HLA-DR is a transmembrane human major histocompatibility complex 2 (MHC II) family member and consists of a 34 kDa (alpha) subunit and one of several 28 kDa (beta) subunits. HLA-DR is expressed primarily by B cells and dendritic cells (DC), in which it binds peptides derived from internalized and processed antigenic proteins. It presents these peptides on the cell surface for recognition by the T cell receptor on CD4+ T cells. This interaction is central to antigen specificity in the adaptive immune response. HLA-DR alleles, polymorphisms, and aberrant expression are linked to a variety of diseases including autoimmunity and cancer.

Long Name
Major Histocompatibility Complex Class II DR
Entrez Gene IDs
3122 (Human)
Alternate Names
FLJ51114; histocompatibility antigen HLA-DR alpha; HLA class II histocompatibility antigen, DR alpha chain; HLADR; HLA-DR; HLA-DRA; HLA-DRA1; major histocompatibility complex, class II, DR alpha; MHC cell surface glycoprotein; MHC class II antigen DRA; MHC Class II DR; MLRW

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Product Specific Notices


This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

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Human HLA-DR Alexa Fluor® 488-conjugated Antibody
By Weifeng Xu on 08/21/2018
Application: Flow Sample Tested: Raji human Burkitt's lymphoma cell line Species: Human