|Detection of HLA‑DR in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) were stained with Mouse Anti-Human CD19 APC‑conjugated Monoclonal Antibody (Catalog # FAB4867A) and either (A) Mouse Anti-Human HLA‑DR Alexa Fluor® 594‑conjugated Monoclonal Antibody (Catalog # FAB4869T) or (B) Mouse IgG1 Alexa Fluor 594 Isotype Control (Catalog # IC002T). View our protocol for Staining Membrane-associated Proteins.|
HLA-DR is a human transmembrane major histocompatibility complex 2 (MHC II) family member that consists of a 34 kDa (alpha) subunit and one of several 28 kDa (beta) subunits. HLA-DR is expressed primarily by B cells and dendritic cells (DC) on which it binds peptides derived from internalized and processed antigenic proteins. It presents these peptides on the cell surface for recognition by the T cell receptor on CD4+ T cells. This interaction is central to antigen specificity in the adaptive immune response. HLA-DR alleles, polymorphisms, and aberrant expression are linked to a variety of diseases including autoimmunity and cancer.
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