Human HLA-DR Alexa Fluor® 750-conjugated Antibody

  • Species Reactivity
  • Specificity
    Detects human HLA‑DR.
  • Source
    Monoclonal Mouse IgG1 Clone # L203
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    RPMI 8866 human lymphoblastoid cells
    Accession # P01903
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
    Alexa Fluor 750
  • Flow Cytometry
    5 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of HLA‑DR in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) were stained with Mouse Anti-Human CD19 PE‑conjugated Monoclonal Antibody (Catalog # FAB4867P) and either (A) Mouse Anti-Human HLA‑DR Alexa Fluor® 750‑conjugated Monoclonal Antibody (Catalog # FAB4869S) or (B) Mouse IgG1 Alexa Fluor 750 Isotype Control (Catalog # IC002S). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: HLA-DR

HLA-DR is a human transmembrane major histocompatibility complex 2 (MHC II) family member that consists of a 34 kDa (alpha) subunit and one of several 28 kDa (beta) subunits. HLA-DR is expressed primarily by B cells and dendritic cells (DC) on which it binds peptides derived from internalized and processed antigenic proteins. It presents these peptides on the cell surface for recognition by the T cell receptor on CD4+ T cells. This interaction is central to antigen specificity in the adaptive immune response. HLA-DR alleles, polymorphisms, and aberrant expression are linked to a variety of diseases including autoimmunity and cancer.

  • Long Name:
    Major Histocompatibility Complex Class II DR
  • Entrez Gene IDs:
    3122 (Human)
  • Alternate Names:
    FLJ51114; histocompatibility antigen HLA-DR alpha; HLA class II histocompatibility antigen, DR alpha chain; HLADR; HLA-DRA; HLA-DRA1; major histocompatibility complex, class II, DR alpha; MHC cell surface glycoprotein; MHC class II antigen DRA; MHC Class II DR; MLRW
Related Research Areas
Isotype Controls
Description Application Cat# Citations Images  

Mouse IgG1 Alexa Fluor® 750-conjugated Isotype Control

Ctrl IC002S
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Staining Reagents
Description Application Cat# Citations Images  

Flow Cytometry Staining Buffer (1X)

Flow FC001 3
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Flow Cytometry Mouse Lyse Buffer (10X)

Flow FC003 1
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Flow Cytometry Human Lyse Buffer (10X)

Flow FC002 1
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