Human HOXB1 Antibody

  
  • Species Reactivity
    Human
  • Specificity
    Detects human HOXB1 in direct ELISAs. In direct ELISAs, less than 2% cross-reactivity with recombinant human (rh) HOXA1, rhHOXB4, and rhHOXA9 is observed.
  • Source
    Polyclonal Sheep IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    E. coli-derived recombinant human HOXB1
    Met1-Thr192
    Accession # P14653
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Immunocytochemistry
    5-15 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Immunocytochemistry
HOXB1 in NTera‑2 Human Cell Line. HOXB1 was detected in immersion fixed NTera‑2 human testicular embryonic carcinoma cells, treated with (upper panel) and without (lower panel) retinoic acid, using Sheep Anti-Human HOXB1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6318) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Preparation and Storage
  • Reconstitution
    Sterile PBS to a final concentration of 0.2 mg/mL.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: HOXB1

HOXB1 (Homeobox protein B1; also HOX-2I) is a 32 kDa (predicted) member of the Antp homeobox family, labial subfamily of transcription factors. It is expressed in rhombomere-4 (r-4) of the hindbrain, where it drives the development of r-4 derived structures. Among these are the motor neurons of the facial nerve, and neural crest cells that form Schwann cells plus cartilage and bone of the neck and ear. Human HOXB1 is 301 amino acids (aa) in length. It contains an Antp-type hexapeptide (aa 179-184) that mediates heterodimerization, and a DNA-binding homeobox domain (aa 203-262). There is one potential splice variant that shows a 40 aa substitution for aa 193-301. Over aa 1-192, human HOXB1 shares 82% aa identity with mouse HOXB1.

  • Long Name:
    Homeobox B1
  • Entrez Gene IDs:
    3211 (Human); 15407 (Mouse); 303491 (Rat)
  • Alternate Names:
    homeo box 2I; homeo box B1; homeobox B1; Homeobox protein Hox-2I; homeobox protein Hox-B1; HOX2; Hox-2.9; HOX2I; HOX2IMGC116844; HOXB1; MGC116843; MGC116845
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