Human HOXB1 Antibody Summary
Accession # P14653
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
HOXB1 in NTera‑2 Human Cell Line. HOXB1 was detected in immersion fixed NTera-2 human testicular embryonic carcinoma cells, treated with (upper panel) and without (lower panel) retinoic acid, using Sheep Anti-Human HOXB1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6318) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
HOXB1 (Homeobox protein B1; also HOX-2I) is a 32 kDa (predicted) member of the Antp homeobox family, labial subfamily of transcription factors. It is expressed in rhombomere-4 (r-4) of the hindbrain, where it drives the development of r-4 derived structures. Among these are the motor neurons of the facial nerve, and neural crest cells that form Schwann cells plus cartilage and bone of the neck and ear. Human HOXB1 is 301 amino acids (aa) in length. It contains an Antp-type hexapeptide (aa 179-184) that mediates heterodimerization, and a DNA-binding homeobox domain (aa 203-262). There is one potential splice variant that shows a 40 aa substitution for aa 193-301. Over aa 1-192, human HOXB1 shares 82% aa identity with mouse HOXB1.
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Before use conjugated 100ug of the HOXB1 antibody (AF6318) with Alexa Fluor 488 using the Alexa Fluor™ 488 Antibody Labeling Kit from ThermoFisher and eluted in 100 μL. 293T cells were transfected with a HOXB1 expressing construct, fixed in 4% paraformaldehyde for 10 minutes at room temperature, and permeabilized/stained using BioLegends True-Nuclear™ Transcription Factor Buffer Set according to the instructions. I stained at 1:800 and got robust staining. Data was acquired on a Cytek Aurora and analyzed in FlowJo.