Human HOXB1 Antibody

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HOXB1 in NTera‑2 Human Cell Line.
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Human HOXB1 Antibody Summary

Species Reactivity
Detects human HOXB1 in direct ELISAs. In direct ELISAs, less than 2% cross-reactivity with recombinant human (rh) HOXA1, rhHOXB4, and rhHOXA9 is observed.
Polyclonal Sheep IgG
Antigen Affinity-purified
E. coli-derived recombinant human HOXB1
Accession # P14653
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.


Recommended Concentration
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunocytochemistry HOXB1 antibody in NTera-2 Human Cell Line by Immunocytochemistry (ICC). View Larger

HOXB1 in NTera‑2 Human Cell Line. HOXB1 was detected in immersion fixed NTera-2 human testicular embryonic carcinoma cells, treated with (upper panel) and without (lower panel) retinoic acid, using Sheep Anti-Human HOXB1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6318) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Sterile PBS to a final concentration of 0.2 mg/mL.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: HOXB1

HOXB1 (Homeobox protein B1; also HOX-2I) is a 32 kDa (predicted) member of the Antp homeobox family, labial subfamily of transcription factors. It is expressed in rhombomere-4 (r-4) of the hindbrain, where it drives the development of r-4 derived structures. Among these are the motor neurons of the facial nerve, and neural crest cells that form Schwann cells plus cartilage and bone of the neck and ear. Human HOXB1 is 301 amino acids (aa) in length. It contains an Antp-type hexapeptide (aa 179-184) that mediates heterodimerization, and a DNA-binding homeobox domain (aa 203-262). There is one potential splice variant that shows a 40 aa substitution for aa 193-301. Over aa 1-192, human HOXB1 shares 82% aa identity with mouse HOXB1.

Long Name
Homeobox B1
Entrez Gene IDs
3211 (Human); 15407 (Mouse); 303491 (Rat)
Alternate Names
homeo box 2I; homeo box B1; homeobox B1; Homeobox protein Hox-2I; homeobox protein Hox-B1; HOX2; Hox-2.9; HOX2I; HOX2IMGC116844; HOXB1; MGC116843; MGC116845

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Human HOXB1 Antibody
By Lucas Armitage on 09/01/2021
Application: Flow Sample Tested: 293T human embryonic kidney cell line Species: Human

Before use conjugated 100ug of the HOXB1 antibody (AF6318) with Alexa Fluor 488 using the Alexa Fluor™ 488 Antibody Labeling Kit from ThermoFisher and eluted in 100 μL. 293T cells were transfected with a HOXB1 expressing construct, fixed in 4% paraformaldehyde for 10 minutes at room temperature, and permeabilized/stained using BioLegends True-Nuclear™ Transcription Factor Buffer Set according to the instructions. I stained at 1:800 and got robust staining. Data was acquired on a Cytek Aurora and analyzed in FlowJo.